The potential of using saliva as a diagnostic fluid is well documented. The aim of this study was to assess the quality and
quantity of saliva DNA of alcoholic and non-alcoholic participants using three saliva collection methods; DNA-SalTM (Oasis
Diagnostics, USA), Oragene-DNA (DNA Genotek Inc, Ontario, Canada) and whole saliva collection method. Saliva DNA
of non-alcoholic (n=30) and alcoholic participants (n=10) age between 25 and 35 years was assessed qualitatively and
quantitatively using spectrophotometry. Saliva DNA quantity was the highest for all participants when using the DNA-Sal TM
saliva collection kit (p<0.05). The use of a mechanical scraper provided only in the DNA-Sal TM kit may have contributed
to the highest DNA yield for all participants. The quantity of saliva DNA when assessed using spectrophotometer was found
to be significantly lower (p<0.05) for the alcoholic (16±3.57 ng/μL) than non-alcoholic participants (19.92±6.18 ng/
μL). To determine the integrity of the DNA samples, PCR amplification of the Alcohol Dehydrogenase gene, ADH1B was
carried out and the PCR was found to be successful. For all participants, the DNA quality of the saliva collected using the
three saliva collection methods was found to be in the acceptable range considered as pure DNA. The DNA quality and
quantity of saliva collected from the three saliva collection methods were considered suitable for research purposes.