Collagen from shortfin scad (Decapterus macrosoma) bone was extracted using varying concentrations of acetic acid. Yields of extracted collagen were 1.01 ± 0.09% and 1.31 ± 0.07% for 0.5 M and 0.7 M acetic acids, respectively. The pH values of commercial collagen and shortfin scad bone collagen extracted using acetic acids (0.5 M and 0.7 M) were 6.52 ± 0.03, 4.99 ± 0.04, and 5.32 ± 0.01, respectively. The functional group analysis for collagen showed that the Amide A, Amide II and Amide III bands have been detected. The microstructure study showed that the isolated collagen had a porous fibril network. Maximum solubility for the commercial collagen was observed at pH 3, while the collagens extracted with 0.5 M and 0.7 M acetic acids were similar at pH 8. Meanwhile, the relative viscosities for all collagens indicated increasing values with increasing temperatures. In conclusion, the shortfin scad bone collagen showed potential for use as an alternative collagen. Although the values for shortfin scad bone collagen extracted by 0.5 M and 0.7 M acetic acids were lower than the commercial one, the collagens exhibited similar properties.