Affiliations 

  • 1 Faculty of Veterinary Medicine, Research Centre for Ruminant Diseases, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  • 2 Faculty of Veterinary Medicine, Research Centre for Ruminant Diseases, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. mzamri@upm.edu.my
  • 3 Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
  • 4 Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bahru, Kelantan, Malaysia
Cytotechnology, 2016 Aug;68(4):1655-9.
PMID: 25511802 DOI: 10.1007/s10616-014-9836-7

Abstract

Monocytes are widely used for immunological research, especially in the study of innate immune system. Although methods for isolation of human monocytes have been established, the procedure for non-human monocyte has not been well developed. This paper describes an improved method for isolation of monocyte and the subsequent macrophage cultivation from caprine blood. Monocytes were isolated from 16 ml of heparinized caprine blood using double density methods; the Ficoll and Percoll. The number of monocytes obtained was 5.12 ± 0.89 × 10(7) cells/ml at 70 % purity. The isolated monocytes were maintained in 10 % fetal bovine serum-enriched Dulbecco's Modified Eagle Medium for maturation to form macrophage cell culture. At the end of the experiment, the harvested macrophage was 2.48 ± 0.33 × 10(6) cells/ml.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.