Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays

Kwong MMY 1 , Lee JW 1 , Samian MR 2 , Watanabe N 3 , Osada H 4 , Ong EBB 5

Affiliations 

  • 1 Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Malaysia; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Malaysia
  • 2 USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Malaysia; School of Biological Sciences, Universiti Sains Malaysia, Malaysia
  • 3 USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Malaysia; Bioprobe Application Research Unit, RIKEN Centre for Sustainable Resource Science, RIKEN, Japan
  • 4 USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Malaysia; Chemical Biology Research Group, RIKEN Centre for Sustainable Resource Science, RIKEN, Japan
  • 5 Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Malaysia; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Malaysia. Electronic address: eugene@usm.my
J Microbiol Methods, 2019 12;167:105743.
PMID: 31629019 DOI: 10.1016/j.mimet.2019.105743

Abstract

This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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