Affiliations 

  • 1 Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Johor, 81310, Malaysia. Electronic address: hasmaliana@utm.my
  • 2 Department of Chemical Engineering and Energy Sustainability, Faculty of Engineering, Universiti Malaysia Sarawak, Malaysia
  • 3 Department of Resource Biotechnology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Malaysia
  • 4 Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, Japan
  • 5 Malaysian Petroleum Resources Corporation (UTM-MPRC), Institute for Oil and Gas, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia
  • 6 Department of Technology and Heritage, Universiti Tun Hussein Onn Malaysia, Malaysia
  • 7 Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Johor, 81310, Malaysia; Institute of Bioproduct Development, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia
J Biotechnol, 2020 Jun 20;317:16-26.
PMID: 32348830 DOI: 10.1016/j.jbiotec.2020.04.011

Abstract

Bacterial pigments are potential substitute of chemical photosensitizer for dye-sensitized solar cell (DSSC) due to its non-toxic property and cost-effective production from microbial fermentation. Serratia nematodiphila YO1 was isolated from waterfall in Malaysia and identified using 16S ribosomal RNA. Characterization of the red pigment produced by the bacteria has confirmed the pigment as prodigiosin. Prodigiosin was produced from the fermentation of the bacteria in the presence of different oil substrates. Palm oil exhibited the best performance of cell growth and equivalent prodigiosin yield compared to olive oil and peanut oil. Prodigiosin produced with palm oil supplementation was 93 mg/l compared to 7.8 mg/l produced without supplementation, which recorded 11.9 times improvement. Specific growth rate of the cells improved 1.4 times when palm oil was supplemented in the medium. The prodigiosin pigment produced showed comparable performance as a DSSC sensitizer by displaying an open circuit voltage of 336.1 mV and a maximum short circuit current of 0.098 mV/cm2. This study stands a novelty in proving that the production of prodigiosin is favorable in the presence of palm oil substrate with high saturated fat content, which has not been studied before. This is also among the first bacterial prodigiosin tested as photosensitizer for DSSC application.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.