Introduction: The aim of this study was to assess the validity and reproducibility of a food frequency questionnaire (FFQ) specifically developed for the assessment of riboflavin intake among Malaysian women in Selangor. Methods: The study was carried out in Universiti Putra Malaysia (UPM), which consisted of 204 healthy women (102 Chinese and 102 Malay) aged between 19 and 45 years. The FFQs were interviewer-administered, which were completed twice; at the beginning of the study (FFQ-1) and two weeks thereafter (FFQ-2). A two-day 24-hour dietary recall (24DR) was interviewer-administered as the standard criteria method. Data were analyzed using Wilcoxon signed ranks test, Spearman correlation coefficient (SCC), intra-class correlation coefficient (ICC) and Bland-Altman plots to determine the validity and reproducibility. Results: Median riboflavin intake derived from 24DRs was significantly higher than FFQ-2 (p