Introduction: Many studies have investigated the knowledge and attitude towards breastfeeding among fathers and mothers, however studies looking at working adults’ knowledge and attitude towards breastfeeding is limited. Work- ing adults with good knowledge and favourable attitude toward the practice of breastfeeding may increase the breast- feeding initiative among working mothers. Therefore, the objective of this study was to investigate the knowledge and attitude toward breastfeeding among staff from the Faculty of Medicine and Health Sciences of a public univer- sity and its associated factors. Methods: A cross sectional study was conducted among faculty staff members. The respondents were selected by simple random sampling and a self-administered questionnaire was used to investigate the knowledge and attitude towards breastfeeding among them. Multiple linear regression was used to determine the factors associated with knowledge and attitude respectively. Results: A total of 117 respondents were recruited to take part in this study. Of these, 83(70.9%) had good knowledge while 61(52.6%) respondents had favourable attitude toward breastfeeding. There was significant positive fair correlation between knowledge and attitude score (r=0.396,p