Endophytes are
an endosymbiont that colonize in plant without causing apparent disease. Medicinal
plan ts have been proven as a host to endophytes fungi thus providing a rich reservoir of bioactive
compounds. However, there is lack information available about medicinal plants associated
endophytes. Therefore, in the present study, the presence of endoph ytic fungi inhabiting in leaves of
Pandanus sp. and Alpinia sp wer e isolated. Healthy and symptomless leaves of Pandanus sp . and
Alpinia sp were surface sterilized and used for the isolation of endophytes. Variation in the
colonization frequency of endop h ytic fungi was observed in leaves of both species. Relatively, leaves
of Pandanus sp . w ere found to have greater colonization frequency ( 16.7 %) as compared to leaves of
Alpinia sp (8.3 %). A total of 3 fungal isolates were obtained and identified based on the morphology
of fungal cultures and spores. Among them one unidentified isolate ( P2) was group to Zygomycota
phylum. Other two isolates were identified as species belong to Lentinus sp ( and C olleotrichum
sp. (P1). Finding in this study suggest the p ossi ble exploration of many such bioactive compounds
from this potential fungus.