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A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens

Wang X 1 , Xiu L 2 , Binder RA 2 , Toh TH 3 , Lee JS 3 , Ting J 3 Show all authors , Than ST 4 , Qi W 1 , Coleman KK 4 , Perera D 5 , Ma M 6 , Gray GC 1

Affiliations 

  • 1 Global Health Research Center, Duke Kunshan University, Kunshan, China
  • 2 Division of Infectious Diseases, Duke University School of Medicine, Durham, NC, USA
  • 3 Faculty of Medicine, SEGi University, Kota Damansara, Selangor, Malaysia
  • 4 Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore
  • 5 Institute of Health and Community Medicine, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia
  • 6 State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100,071, China
One Health, 2021 Dec;13:100274.
PMID: 34124332 DOI: 10.1016/j.onehlt.2021.100274

Abstract

We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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