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Designing a highly immunogenic multi epitope based subunit vaccine against Bacillus cereus

Asif Rasheed M 1 , Awais M 2 , Aldhahrani A 3 , Althobaiti F 4 , Alhazmi A 5 , Sattar S 1 Show all authors , Afzal U 6 , Ali Baeshen H 7 , Ali El Enshasy H 8 , Joe Dailin D 8 , Al-Surhanee AA 9 , Kabir F 10

Affiliations 

  • 1 Department of Biosciences, COMSATS University Islamabad, Sahiwal Campus, 57000 Sahiwal, Pakistan
  • 2 Department of Biochemistry and Molecular Biology, Faculty of Science, University of Sialkot, Pakistan
  • 3 Clinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi Arabia
  • 4 Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
  • 5 Medical Laboratory Technology Department, Jazan University, Jazan, Saudi Arabia
  • 6 Department of Chemistry, Rawalpindi Women University, Satellite Town Rawalpindi, Pakistan
  • 7 Department of Orthodontics, Faculty of Dentistry, King Abdulaziz University, P. O. Box 80209, Jeddah 21589, Saudi Arabia
  • 8 Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor, Malaysia
  • 9 Biology Department, College of Science, Jouf University, Sakaka 2014, Kingdom of Saudi Arabia
  • 10 University Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan
Saudi J Biol Sci, 2021 Sep;28(9):4859-4866.
PMID: 34466059 DOI: 10.1016/j.sjbs.2021.06.082

Abstract

Objective: Serious non-gastrointestinal-tract infections and food poisoning are caused by Bacillus cereus. Vaccination against B. cereus is very important. The aim of this study was to identify and analyze B and T cell epitopes for chromate transporter protein of the bacteria.

Methods: Multiple sequence alignment with the Clustal Omega method was used to identify conserved regions and Geneious Prime was used to produce a consensus sequence. T and B cell epitopes were predicted by various computational tools from the NetCTL and Immune Epitope Database (IEDB), respectively.

Results: Altogether, 6 HTL cells and 11 CTL epitopes were predicted. This vaccine's molecular docking is done with Patch Dock and LigPlot to verify interactions. The immune server (C-IMMSIM) was used to develop In silico immune response in order to assess the multi-epitope vaccine's immunogenic profile.

Conclusion: We designed universal vaccine against B. cereus responsible for food poisoning. The disease may be avoided with the aid of the proposed epitope-based vaccine.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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