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Pomegranate peels waste hydrolyzate optimization by Response Surface Methodology for Bioethanol production

Chaudhary A 1 , Hussain Z 1 , Aihetasham A 2 , El-Sharnouby M 3 , Abdul Rehman R 4 , Azmat Ullah Khan M 5 Show all authors , Zahra S 6 , Saleem A 1 , Azhar S 2 , Alhazmi A 7 , El Askary A 8 , Sayed S 9 , Ali El Enshasy H 10 , Zulaiha Hanapi S 10 , Qamer S 11

Affiliations 

  • 1 Department of Zoology, Division of Science and Technology, University of Education, Lahore 54770, Pakistan
  • 2 Institute of Zoology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan
  • 3 Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
  • 4 Department of Forensic Sciences, University of Health Sciences, Lahore, Pakistan
  • 5 Department of Biochemistry and Molecular Biology, University of Gujrat, Hafiz Hayat campus. Gujrat, Pakistan
  • 6 Department of Physics, Division of Science and Technology, University of Education, Lahore 54770, Pakistan
  • 7 Medical Laboratory Technology Department, Jazan University, Jazan, Saudi Arabia
  • 8 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
  • 9 Department of Science and Technology, University College-Ranyah, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
  • 10 Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor, Malaysia
  • 11 Department of Zoology, Government College University, Faisalabad, Pakistan
Saudi J Biol Sci, 2021 Sep;28(9):4867-4875.
PMID: 34466060 DOI: 10.1016/j.sjbs.2021.06.081

Abstract

Unwanted agricultural waste is largely comprised of lignocellulosic substrate which could be transformed into sugars. The production of bioethanol from garbage manifested an agreeable proposal towards waste management as well as energy causation. The goal of this work is to optimize parameters for generation of bioethanol through fermentation by different yeast strains while Saccharomyces cerevisiae used as standard strain. The low cost fermentable sugars from pomegranate peels waste (PPW) were obtained by hydrolysis with HNO3 (1 to 5%). The optimum levels of hydrolysis time and temperature were elucidated via RSM (CCD) ranging from 30 to 60 min and 50 to 100 °C respectively. The result shows that optimum values (g/L) for reducing sugars was 61.45 ± 0.01 while for total carbohydrates was 236 ± 0.01. These values were found when PPW was hydrolyzed with 3% HNO3, at 75 °C for one hour. The hydrolyzates obtained from the dilute HNO3 pretreated PPW yielded a maximum of 0.43 ± 0.04, 0.41 ± 0.03 g ethanol per g of reducing sugars by both Metchnikowia sp. Y31 and M. cibodasensis Y34 at day 7 of ethanologenic experiment. The current study exhibited that by fermentation of dilute HNO3 hydrolyzates of PPW could develop copious amount of ethanol by optimized conditions.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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