Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

Yeap CSY 1 , Chaibun T 1 , Lee SY 2 , Zhao B 3 , Jan Y 3 , La-O-Vorakiat C 4 Show all authors , Surareungchai W 4 , Song S 3 , Lertanantawong B 1

Affiliations 

  • 1 Biosensors Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom, Thailand. benchaporn.ler@mahidol.ac.th
  • 2 Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio) and Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Kedah, 08100, Malaysia
  • 3 Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China
  • 4 Pilot Plant and Development Training Institute, Nanoscience and Nanotechnology Research Cluster, King Mongkut's University of Technology Thonburi (KMUTT), Bang Khun Thian Campus, Bangkok 10150, Thailand
Chem Commun (Camb), 2021 Nov 16;57(91):12155-12158.
PMID: 34726213 DOI: 10.1039/d1cc05181d

Abstract

We report a highly sensitive and selective multiplex assay by empowering an electrochemical DNA sensor with isothermal rolling circle amplification. The assay could simultaneously detect and discriminate three common entero-pathogens in a single reaction, with femtomolar sensitivity. It is useful for field- or resource-limited settings.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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