Affiliations 

  • 1 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang 11800, Malaysia
  • 2 Department of Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Wildau 15745, Germany
  • 3 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang 11800, Malaysia. Electronic address: theamsoon@usm.my
Anal. Biochem., 2014 Oct 15;463:67-9.
PMID: 24972268 DOI: 10.1016/j.ab.2014.06.012

Abstract

Immunoassays are often coupled to peroxidase activity for antigen detection. Sensitivity and speed of detection has been increased by the advent of hybrid methods such as immuno-PCR (polymerase chain reaction). However, a more simplified immunoassay that retains both colorimetric peroxidase detection and effective DNA amplification in a setting closer to field application conditions has been nonexistent. Here we describe a method that successfully combines a competitive immunoassay with the new isothermal quadruplex-primed amplification (QPA) to generate excess quadruplex reporter molecules with intrinsic peroxidase DNAzyme activity.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.