MATERIALS AND METHODS: A UV-visible spectrophotometer and SEM were used to characterize the green synthesized SeNPs. The anti-inflammatory and anti-diabetic activities of green synthesized SeNPs were measured using the alphaamylase inhibitory & beta-glucosidase enzyme inhibition assay and the egg albumin, bovine serum albumin, and membrane stabilization assays. A test for the mortality of brine shrimp was used to determine the cytotoxic impact of SeNPs.

RESULTS: A. linearis powder was used for the green synthesis of selenium nanoparticles, which exhibited the highest peak at 440 nm when analyzed using a UV-visible spectrophotometer. The In vitro anti-inflammatory effect of synthesized SeNPs was maximally inhibited by 44-83% in the bovine serum albumin assay 54-79% in the egg albumin assay, and 54-86% in the membrane stabilization assay compared with standard. The inhibition percentage of antidiabetic activity was found to be 50-86% in the alphaamylase assay and 49-85% in the beta-glucosidase assay when compared to standards at various concentrations. Furthermore, the cytotoxicity impact shows that 70% of brine shrimp were alive at the maximum fixation of 80 µg/mL.

MATERIALS AND METHODS: Biogenically synthesized silver nanoparticles (Ag NPs) from an herbal formulation containing Zingiber officinale and Ocimum gratissimum were tested at various concentrations using the DPPH (2,2- diphenyl-1-picrylhydrazyl) assay. The absorbance was measured at 517 nm to quantify DPPH free radicals. With Ag NP concentrations, the H2O2 test exhibited increased activity. This work evaluated the antibacterial activity of Ag NPs mediated by Zingiber officinale and Ocimum gratissimum against Staphylococcus aureus, Streptococcus mutans, Candida albicans, and Enterococcus faecalis.

RESULTS: The utilization of herbal formulations from Z. officinale and O. gratissimum to synthesize Ag NPs revealed considerable therapeutic effectiveness. At a concentration of 50 μl, the maximal inhibition was 76%, which is comparable in effectiveness to that of standard ascorbic acid. Significant blood clot dissolution was observed during thrombolytic testing at a concentration of 100μg/ml, indicating promising prospects for the treatment of thrombotic disorders. Nanoparticles dose-dependently inhibited E. faecalis, C. albicans, S. aureus, and S. mutans in antibacterial testing. These results show the potential of the nanoparticles as supplementary or alternative treatments to conventional antibiotics, particularly in light of the increasing prevalence of antibiotic resistance.