Displaying all 3 publications

Abstract:
Sort:
  1. Urogenital myiasis caused by Chrysomya bezziana (Diptera: Calliphoridae) in peninsular Malaysia
    Ramalingam S, Nurulhuda A, Bee LH
    Southeast Asian J Trop Med Public Health, 1980 Sep;11(3):405-7.
    PMID: 7444582
    A case of urogenital myiasis caused by Chrysomya bezziana (Diptera: Calliphoridae) was diagnosed in a 76-year old patient who had carcinoma of the rectum. A total of 35 larvae were obtained from ulcers near the external genitalia and urethra opening. Larvae pupated within 1 to 2 days and 6 days later emerged as adult males. These were identified as Chrysomya bezziana. Female flies possibly attracted by the fetid odour, laid eggs in the existing lesions in the urogenital area, the larvae invading and feeding on living tissue. Lack of personal hygiene was the contributing factor for the cause of urogenital myiasis in this patient.
  2. Optimization of agarose-alginate hydrogel bead components for encapsulation and transportation of stem cells
    Xiang Ping MK, Zhi HW, Aziz NS, Hadri NA, Ghazalli NF, Yusop N
    J Taibah Univ Med Sci, 2023 Feb;18(1):104-116.
    PMID: 36398016 DOI: 10.1016/j.jtumed.2022.08.009
    Hydrogels have potential uses in various biological applications because of their unique characteristics. Fine-tuning of agarose-alginate (Ag-Al) hydrogel components improves the mechanical characteristics of the final construct for cell encapsulation and transportation. Formulation of suitable dissolving agents may enable the release of encapsulated cells for further applications in laboratory or clinical settings.

    Objectives: This study aimed at optimizing the composition of Ag-Al hydrogel beads and their dissolving agents for potential use in the transportation of stem cells.

    Methods: Various agarose, alginate, and CaCl2 concentrations were tested to construct hydrogel beads. The degradation rate and swelling ratio of each hydrogel sample were recorded. The optimized Ag-Al hydrogels were used for encapsulation of stem cells from human exfoliated deciduous teeth (SHED). Optimization of dissolving agents was performed and tested with the hydrogel-encapsulated cells. Data were statistically analyzed in SPSS.

    Results: The selected concentration of Ag-Al hydrogels components was successfully demonstrated to encapsulate SHED, which remained viable until day 10. An average of 2 min was required for degradation of the hydrogel with encapsulated SHED by a dissolving agent consisting of 100 mM sodium citrate and 100 mM EDTA. The cell viability of SHED released after day 10 of encapsulation was 29.1%.

    Conclusion: Alteration of Ag-Al components has considerable influence on the mechanical properties of the constructed hydrogel. The feasibility of performing the optimized cell encapsulation protocol, as well as the dissolving step, may provide a useful guide for the transportation of viable cells between countries, for medical research.

  3. Fulltext Retrospective prevalence of snakebites from Hospital Kuala Lumpur (HKL) (1999-2003)
    Jamaiah I, Rohela M, Ng TK, Ch'ng KB, Teh YS, Nurulhuda AL, et al.
    Southeast Asian J Trop Med Public Health, 2006 Jan;37(1):200-5.
    PMID: 16771235
    A hospital based retrospective study of the prevalence of snakebite cases at Hospital Kuala Lumpur was carried out over a five-year period from 1999 to 2003. A total of 126 snakebite cases were recorded. The highest admission for snakebites was recorded in 2001 (29 cases). The majority of cases were admitted for three days or less (79%). Most of the snakebite cases were reported in the 11-30 years age group (52%). The male:female ratio was 3:1. The majority of cases were Malaysians (80%, 101 cases). Of the non-Malaysians, Indonesians constituted the most (56%, 14 cases). Bites occurred most commonly on the lower limbs (49%), followed by upper limbs (45%) and on other parts of the body (6%). No fatal cases were detected and complications were scarce. In 60% (70 cases) the snake could not be identified. Of the four species of snakes that were identified, cobra (both suspected and confirmed) constituted the largest group (25%), followed by viper (10%), python (4%) and sea snake (1%). The most common clinical presentations were pain and swelling, 92% (116 cases). All patients were put on snakebite charts and their vital signs were monitored. Of the snakebite cases, 48% (61 cases) were treated with cloxacillin and 25% (32 cases) were given polyvalent snake antivenom.
Related Terms
    Try searching for something
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links