Dengue is endemic in Kolkata, India. Outbreaks of dengue cases often occur regularly at short intervals. This
retrospective seroepidemiological surveillance was conducted longitudinally. Dengue cases were diagnosed
in the laboratories from suspected patients by dengue specific IgG, IgM antibodies and NS1 antigen, from
suspected persons to investigate, analyze and categorize the cases who were actually suffering from dengue to
diagnose a dengue patient in the laboratory practice along with the status of the patient related to the detection
of disease and duration of primary and secondary infection for effective monitoring of the patient. Age and
Sex of the dengue patients were determined. Detection of dengue in unsuspected fever cases in unfavorable
transmission season was evaluated. The transmission of dengue infection in the non-transmission season is
not remarkable and often remains submerged. Proper measure at this stage may prevent the epidemic
outbreak in the transmission season. This sort of experience will help to enrich the effective control and case
management of the menace.
Introduction: The problem in patients who are intubated with endotracheal tube is the occurrence of accumulation of secretions. One of the actions to reduce the secretion is closed suction. This research monitors the patient's oxy- gensaturation and also reveals the effect of closed suction on oxygen and AaDO2 saturation (alveolar-arterial oxygen difference) in patients who were mechanically ventilated in the Intensive Care Unit (ICU) of Dr. Kariadi Hospital. The design of this study uses pre experiment. Methods: The research process has been carried out on April 2, 2019 until May 26, 2019 in the Intensive Care Unit with a total sampling technique, on 15 respondents. Result: The results of the study showed that there was effect of closed suction technique on the value of oxygen saturation in patients with mechanical ventilation (p-value = 0.010). Conclusion: Closed suction can lead to decrease of air volume in the lungs up to 1200 ml. So hyperoxygenation must be done before closed suction as it can reduces the incidence of hypox- emia by 49%. Before closing the suction, it is recommended to do hyperoxygenation.