MyMedR

Displaying all 3 publications

Abstract:

Sort:

Fulltext Involvement of intracellular calcium and MAPK in aloe emodin induced anti-proliferative in MCF-7

Indah M Amin, Mohd Ridzuan Hamid, Dayang Zahidah A. Othman, Rosfaiizah Siran, Siti Hamimah S.A. Kadir, Narimah AH Hasani

ASM Science Journal, 2014;8(2):165-173.
MyJurnal

Aloe emodin, an anthraquinone of Aloe barbadensis Miller has been shown to have more cytotoxic effect in
different kinds of human cancer cell lines compared to normal. Accordingly, we found it to selectively inhibit
the proliferation of oestrogen-receptor-positive-(ER+)-breast cancer cells, MCF-7; but not controls cells,
MCF-10A. However, its precise mechanism is not well understood. Several studies have shown that there is
evidence of increased intracellular calcium (Ca2+), both at early and late stage of apoptosis which associated
with the down-regulation of ERK1/2 proliferative pathway. Therefore, we aim to elucidate the involvement
of intracellular Ca2+ in aloe emodin induced apoptosis on MCF-7. Apoptotic morphological changes were
observed under fluorescence microscope. The involvement of cytoplasmic Ca2+ and MAPKs were investigated
using Fluo-4 intracellular Ca2+ imaging and QuantiGene 2.0 Plex assay, respectively. IC50 of aloe emodin
(80 μM) at 72 hours incubation was used. Data were evaluated using the one-way or two-way ANOVA tests.
Our results indicated that aloe emodin at IC50 80µM induced apoptosis on MCF-7 through the association of
intracellular Ca2+ signalling. This observation include a significant increased (p
Fulltext Recombinant proteins from new constructs of SAG1 and GRA7 sequences and their usefulness to detect acute toxoplasmosis

Kotresha D, Poonam D, Muhammad Hafiznur Y, Saadatnia G, Nurulhasanah O, Sabariah O, et al.

Trop Biomed, 2012 Mar;29(1):129-37.
PMID: 22543613 MyJurnal

In this study we have cloned unreported gene fragments of Toxoplasma gondii GRA7 and SAG1 and expressed the corresponding recombinant proteins, followed by evaluation of their usefulness for the serological diagnosis of toxoplasmosis. Both recombinant proteins were expressed efficiently in insoluble form, purified by single step Ni-NTA affinity chromatography and their antigenicity to detect toxoplasma specific IgG antibodies were determined by immunoblotting. A total of 60 serum samples from three groups of individuals based on their anti-toxoplasma antibody profiles were tested, namely (I) IgM+, IgG+ (n=20), (II) IgM-, IgG+ (n=20) and (III) IgM-, IgG- (n=20). Both recombinant proteins exhibited high sensitivity (100%) with sera from Group I. rGRA7 and rSAG1 reacted 40% and 80% respectively with Group II sera. The specificity of the recombinant proteins based on reactivities with Group III sera were 100% and 80% with rGRA7 and rSAG1 respectively. Thus rGRA7 was found to be better at discriminating probable acute from chronic phases of toxoplasmosis, and it also showed higher specificity.
COVID-19 vaccine safety and side effects in children aged 5-11 years: a cross-sectional study

Nurhani MA, Farah HMS, Ili NMA, Zahidah AR, Rahimah B, Nabilah HK, et al.

Med J Malaysia, 2023 Nov;78(6):803-807.
PMID: 38031224

INTRODUCTION: The COVID-19 pandemic has prompted a global drive for vaccination, including children. Despite the urgency, understanding the safety and side effects remains crucial. Our study aimed to evaluate the safety of the Pfizer- BioNTech (BNT162b2) vaccine in children by determining the proportion of vaccinated children who experienced side effects and identifying factors associated with postvaccination side effects.
MATERIALS AND METHODS: A cross-sectional study was conducted among children who received the COVID-19 vaccine between 3 February and 8 May 2022. Data were collected using a self-administered questionnaire filled out by the parent or legal guardian.
RESULTS: The mean age of the study participants was 9 years old and 43.1% were males. Out of the 195 participants in the study, 62 (31.8%) reported side effects after vaccination. The most frequently reported side effects were pain at the injection site (29.7%, n=58), fever (15.9%, n=31), localised inflammation (10.8%, n=21) and arthralgia/myalgia (9.2%, n=18). There were no reported severe adverse events such as anaphylaxis or myocarditis. Most side effects occurred within the first two days post-vaccination. There was a higher proportion of side effects among children with underlying co-morbidities. No significant differences were observed based on age, weight, ethnicity and the presence of allergies, or the use of premedication.
CONCLUSION: The BNT162b2 vaccine was generally welltolerated in children, with most side effects being mild and self-limiting. These findings support the safety of the COVID-19 vaccine and would guide healthcare professionals, parents and policy-makers in making informed decisions about COVID-19 vaccination, especially among high-risk groups.

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links