MyMedR

Fulltext Vector and reservoir host of a case of human Brugia pahangi infection in Selangor, peninsular Malaysia

Muslim A, Fong MY, Mahmud R, Sivanandam S

Trop Biomed, 2013 Dec;30(4):727-30.

A case of human eye infection caused by Brugia pahangi was reported in 2010 in a semi rural village in Selangor, peninsular Malaysia. Our report here reveals results of investigation on the vector and animal host for the transmission of the infection. We conducted entomological survey and cat blood examination in the vicinity of the patient's home. The mosquito species Armigeres subalbatus was incriminated as the vector, whereas cat served as the reservoir host.

Adult Wuchereria bancrofti (Cobbald, 1877) in subcutaneous cyst of man

Sandosham AA, Sivanandam S

Med J Malaya, 1968 Mar;22(3):236.

PMID: 4234372

Filariasis in Negri Sembilan--a follow-up study

Cheah WC, Cheong WH, Mahadevan S, Lai KP, Sivanandam S

Med J Malaysia, 1977 Dec;32(2):103-10.

PMID: 614475

Diurnally sub-periodic microfilarial pattern in Brugia malayi in West Malaysia

Dondero TJ, Sivanandam S

Trans R Soc Trop Med Hyg, 1971;65(5):691-3.

PMID: 5159155

A new filarial worm in the heart of a forest rat (Rattus sabanus)

Sandosham AA, Sivanandam S

Med J Malaya, 1966 Jun;20(4):339-40.

PMID: 4224352

A double infection of filariasis in a black panther (Panthera pardus) from Pahang

Strauss JM, Sivanandam S

Med J Malaya, 1966 Jun;20(4):336.

PMID: 4224351

Development of Wuchereria bancrofti in the domestic cat

Ramachandran CP, Sandosham AA, Sivanandam S

Med J Malaya, 1966 Jun;20(4):333.

PMID: 4224348

Fulltext Recent advances on the use of biochemical extracts as filaricidal agents

Al-Abd NM, Nor ZM, Al-Adhroey AH, Suhaimi A, Sivanandam S

Evid Based Complement Alternat Med, 2013;2013:986573.

PMID: 24298292 DOI: 10.1155/2013/986573

Lymphatic filariasis is a parasitic infection that causes a devastating public health and socioeconomic burden with an estimated infection of over 120 million individuals worldwide. The infection is caused by three closely related nematode parasites, namely, Wuchereria bancrofti, Brugia malayi, and B. timori, which are transmitted to human through mosquitoes of Anopheles, Culex, and Aedes genera. The species have many ecological variants and are diversified in terms of their genetic fingerprint. The rapid spread of the disease and the genetic diversification cause the lymphatic filarial parasites to respond differently to diagnostic and therapeutic interventions. This in turn prompts the current challenge encountered in its management. Furthermore, most of the chemical medications used are characterized by adverse side effects. These complications urgently warrant intense prospecting on bio-chemicals that have potent efficacy against either the filarial worms or thier vector. In lieu of this, we presented a review on recent literature that reported the efficacy of filaricidal biochemicals and those employed as vector control agents. In addition, methods used for biochemical extraction, screening procedures, and structure of the bioactive compounds were also presented.

Filariasis in a rubber plantation in Negeri Sembilan, West Malaysia

Ramachandran CP, Dondero TJ, Mullin SW, Sivanandam S, Stevens S

Med J Malaya, 1971 Jun;25(4):273-7.

PMID: 4261299

Fulltext Modified Field stain - rapid viability test for Trichomonas vaginalis

Afzan MY, Sivanandam S, Suresh K

J Appl Microbiol, 2012 Jan;112(1):132-7.

PMID: 22040369 DOI: 10.1111/j.1365-2672.2011.05185.x

We previously reported that Modified Field Stain (MF) can be used as a rapid stain for diagnosis. In the present study we extend the observation to include the stain as an alternative method to assess viability of the cells.

Fulltext Pathogenesis of Plasmodium berghei ANKA infection in the gerbil (Meriones unguiculatus) as an experimental model for severe malaria

Junaid QO, Khaw LT, Mahmud R, Ong KC, Lau YL, Borade PU, et al.

Parasite, 2017;24:38.

PMID: 29034874 DOI: 10.1051/parasite/2017040

BACKGROUND: As the quest to eradicate malaria continues, there remains a need to gain further understanding of the disease, particularly with regard to pathogenesis. This is facilitated, apart from in vitro and clinical studies, mainly via in vivo mouse model studies. However, there are few studies that have used gerbils (Meriones unguiculatus) as animal models. Thus, this study is aimed at characterizing the effects of Plasmodium berghei ANKA (PbA) infection in gerbils, as well as the underlying pathogenesis.

METHODS: Gerbils, 5-7 weeks old were infected by PbA via intraperitoneal injection of 1 × 106 (0.2 mL) infected red blood cells. Parasitemia, weight gain/loss, hemoglobin concentration, red blood cell count and body temperature changes in both control and infected groups were monitored over a duration of 13 days. RNA was extracted from the brain, spleen and whole blood to assess the immune response to PbA infection. Organs including the brain, spleen, heart, liver, kidneys and lungs were removed aseptically for histopathology.

RESULTS: Gerbils were susceptible to PbA infection, showing significant decreases in the hemoglobin concentration, RBC counts, body weights and body temperature, over the course of the infection. There were no neurological signs observed. Both pro-inflammatory (IFNγ and TNF) and anti-inflammatory (IL-10) cytokines were significantly elevated. Splenomegaly and hepatomegaly were also observed. PbA parasitized RBCs were observed in the organs, using routine light microscopy and in situ hybridization.

CONCLUSION: Gerbils may serve as a good model for severe malaria to further understand its pathogenesis.

The size of microfilariae in relation to the storage time of unfixed and fixed thick blood films

Sivanandam S, Yap Loy Fong

Med J Malaya, 1965 Sep;20(1):63-4.

PMID: 4221424

Displaying publications 21 - 32 of 32 in total