Perceived Benefits and Risks: A survey data set towards Wolbachia-infected Aedes Mosquitoes in the Klang Valley, Malaysia. Introduction: The paper presents data collected using measures of perceived benefits, perceived risks, trust in key players, attitude towards nature versus material, attitude towards technology, religiosity, and attitude towards the Wolbachia-infected Aedes mosquitoes (WiAM) technique. The validated questionnaires were used to randomly survey targeted stakeholders in the Klang Valley, Malaysia, who had been asked to voluntarily participate in face-to-face interviews. Completed questionnaires were received from 399 respondents (adults above 18 years old) and comprised two stakeholder groups: scientists (n = 202), and the public (n = 197). The detailed findings serve numerous opportunities to examine the social acceptance of Wolbachia-infected Aedes mosquitoes, to ensure the development of policy and action plans, and to encourage further study by other researchers interested in the measures and data presented.
Metals are natural elements existed in the environment. However, due to the rapid development of urbanisation and economic, high content of anthropogenic metals are being perceived in polluting the environment. The oceans are known to be a part of the sinking basin for anthropogenic metals ends. Dataset provided is purposely to give an overview of dissolved metals spatial distribution in the South China Sea off the east Peninsular of Malaysia during the pre-, post- and Northeast (NE) Monsoon period. Seawater samples were collected in a grid of 18 stations at 3 different water depth. Dissolved metals were pre-concentrated on-board ship using Chelex-100 resin and analysed using Inductively Coupled Plasma Mass Spectrophotometry (ICPMS). The dataset shows the effect of NE Monsoon on dissolved metals spatial distribution mainly at the area closer to the land. Therefore, this dataset could reveal the past information on anthropogenic metals intrusion in the South China Sea, since Terengganu state was recently pointed to be one of the Malaysian waterfront city. Additionally, this dataset also could help in studying the cycle of metals in the southern South China Sea waters.
The Transient Electro-Magnetic (TEM) geophysical technique was deployed to map and characterized the subsurface of Pahang River Basin along the East Coast Peninsula Malaysia. The data aimed at differentiating between the massive zones and the weak zones within the region, to also assess and differentiate the subsurface structures and comes up with recommendations for policy decision, formulation and plans on the flooding impact, surface water and groundwater managements, in addition to other environmental related issues ravaging the area. The data presented in this paper, showed the properties of the subsurface rocks underlain the region as beneficial to the Agriculturists; Climatologists; Engineers; Environmentalists; Geoscientists, Hydrologists and Policy formulation officers. The TEM data collection utilized a 100 m x 100 m single loop coil for both the Transmitter (Tx) loop and the Receiver (Rx) loop to produce a total surface area coverage of 10,000 m2 per survey line along a single profile. The total area covered in the data extended across an average area of 30 km x 40 km in parts of Maran, Temerloh and Jerantut districts, within the State of Pahang, East Coast, Peninsula Malaysia. The conductivity data recorded varied from -20 mS/m to about 440 mS/m at a maximum depth of about 375 m. On the other hand, the resistivity data recorded varied from 0 Oh-m to about 1000 Oh-m. The information derived from the data are intended for potential abstraction by the Malaysian Groundwater Management Board; the Department of Mineral and Geoscience; Department of Irrigation and Drainage; the Pahang State Water Board, and the Department of Agriculture.
Industrial, and municipal wastes are part of the main sources of environmental hazards as well as groundwater and surface water pollutions. If not well composed, treated, and safely disposed, it could permeate through the subsurface lithologies by reaching down to the underground water aquifers, particularly in zones of unprotected aquifer units. Pollutants, most especially the landfills leachates that encompassed organic contaminants, ammonia, nitrates, total nitrogen, suspended solids, heavy metals and soluble inorganic salts, i.e., soluble nitrogen, sulphur compound, sulphate and chlorides, could posed undesirable environmental impacts due to inappropriate disposals that may give rise to gaseous fumes and leachate formations. An electrical resistivity geophysical technique utilizing the RES2D no-invasive, cost-effective and rapid method of data collection was integrated with the 3D Oasis Montaj software to approximate the volume of the generated rectangular prism model of the contaminants delineated from mixtures of the industrial, and municipal wastes plumes to be 312,000 m 3.
The paper presents the data from the surface and subsurface mapping of this area for the purpose of siting industrial city in the area. The field data collected combine with the borehole data was to successfully apply these to solving geological, environmental and engineering complications posed by the complexity of the subsurface geological structures underlain this area. The Electrical Resistivity, (ER) and Induced Polarization, (IP) data were initially processed using RES2DINV software model to generate the depth to the lithological units together with topographic correction. The 2-D ER and IP data were collected from 23rd April 2017 up until 7th May 2017 covering a total of about 17.6 km along 44 survey lines using ABEM Terrameter SAS4000 for the field measurement. A total of 20 Borehole logs data were recorded to better characterized in-situ, the subsurface geological formations emplaced in the study area. The study area is located at Bagan Datuk, Perak Darul Ridzuan situated on Latitude 2° 44.653'N and Longitudes 104° 28.79' E along the west coast Peninsula Malaysia. The topography of the area is generally flat low-laying and elevation range from about 0 m to 32 m above mean sea level (MSL).
To identify the potential zones for gold mineralization at the Felda Chiku 3, Gua Musang, Kelantan, East coast Malaysia, twenty-one (21) geophysical survey lines were conducted at the proposed mineral exploration site using the pole - dipole of the electrical resistivity and induced polarization arrays to get the maximum depth of 150 m with 400 m survey length. From the resistivity and chargeability concentration maps, the potential mineralized zones as delineated, was observed to be dominantly concentrated towards the southwest and northern part of the area. The 3D resistivity and chargeability slice model present low resistivity values and high chargeability values that are well correlated which is palpable especially at the depths of 25 m and 50 m respectively. The data showed that the potential mineralized zones are trending approximately north-south directions. Forty (40) drilling locations were proposed for follow-up drilling based on the resistivity and chargeability models.
This paper presents data on nitrogen characteristics in a tropical seagrass meadow located in Sungai Pulai estuary (Johor, Malaysia) and is related to the article "Nitrogen dynamics in tropical seagrass meadows under heavy anthropogenic influence" [1]. Field sampling conducted from August 2015 to May 2016 aimed to collect sediments and seagrass tissues for analysis of nitrogen elemental content and stable isotope values. Sediment samples and seagrass tissue (above-ground and below-ground parts) were collected by using PVC cores. The information is presented as unprocessed and partially data, which incorporates nitrogen content (in %) and δ15N values (‰) of sediment and seagrass tissue samples. Nitrogen loadings in the seagrass sediments, as based on down-core data of sediment samples up to 30 cm depths, should be read with [1] to comprehend the baseline nitrogen dynamics of the study area.
Deer antler velvet (DAV) has been traditionally used in Chinese medicine, including treatment on toothache [1]. Due to its rapid and regenerative capacity, deer antlers were proposed to be the good model for bone remodelling in mammals [2]. The data presented in this work is on the liquid chromatography and mass spectrometry (LC-MS) profile and bioactive potential of Malayan deer antler velvet (DAV) on different Candida species that has clinical importance. Aqueous extraction of DAV samples was subjected to Liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF-MS) profiling. Reverse phase (RP) separation was used due to the process extraction using water as a solvent to separate polar compound. The data was interpreted using Profile Analysis 2.1V. The DAV samples were also tested for the effect on the biofilm formation of seven Candida species in a 96 well plate [3]. The biofilms were developed for 72 h in aerobic environment. Following that, the biofilms biomass was determined using crystal violet assay.
Metagenomic datasets of the microbial DNA of workers of a Pheidole decarinata Santschi (Hymenoptera: Formicidae) around houses with three replicates were presented. Next-generation sequencing of the microbial DNA was performed on an Illumina Miseq platform. QIIME (version 1.9.1) was used to analyze the raw fastq files. Metagenome of the three (3) samples consist of 333,708 sequences representing 137,359,149 bps with an average length of 413.67 bps. The sequence data is available at the NCBI SRA with the bioproject number PRJNA632430. Community analysis revealed Proteobacteria was the predominant (84.77%) microbial community present in the microbial DNA of workers of the P. decarinata.
This article contains the data set and model code for the negative emission polygeneration system described in Tan et al. (2019). The data was generated utilizing an optimization model implemented in LINGO 18.0 and includes information on the operating state of each process unit in the system. The maximum annual profit of the system was determined at different carbon footprint targets. The data set and model code can be utilized for further analysis on the interdependence between the process units of this polygeneration system, its operational and environmental performance, and the potential impact of integrating new process units into the network.
The dataset includes the particulars of 515 respondents on safety behavior during COVID-19. The questionnaires were adapted using Social Learning Theory and Social Exchange Theory. The variables included in dataset are Transactional Leadership (TSL), Transformational Leadership (TFL), Employee Well-Being (EWB) and Safety behavior (SB). Moreover, the dataset also contains the demographic profile of the respondents. Data was collected with the help of self-administered questionnaire from eight public hospitals in Punjab, Pakistan, namely Services Hospital Lahore, Sir Ganga Ram Hospital Lahore, Government General Hospital Faisalabad, DHQ Hospital Chiniot, Municipal General Hospital Sargodha, DHQ Hospital Jhang, DHQ Hospital Multan and Sulehri Children & General Hospital Sialkot. This dataset could provide a significant insight for future research in employee safety behavior.
The article presents data on the quantification of gallic acid (GA) and the assessment of the antioxidant and anti-inflammatory properties of Kacip Fatimah (Labisia pumila Benth. & Hook. f.) leaves using various solvents. GA was quantified using high-performance liquid chromatography analysis. Total phenolic content (TPC) was assessed using the Folin-Ciocalteu method. The antioxidant activities were evaluated using xanthine oxidase superoxide (XOD-Superoxide) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, while anti-inflammatory activities were examined through lipoxygenase (LOX) and xanthine oxidase (XOD) inhibition assays. Results showed that the water-extracted sample had the highest GA and TPC among the solvents tested, along with the strongest inhibition activities in the XOD-Superoxide and DPPH assays. Both water and ethanol extracts showed significant inhibitory activities in the LOX assay but were inactive in the XOD assay. These findings suggest that the bioactivity of L. pumila leaf extract is associated with GA and TPC. GA and TPC strongly correlated with antioxidant and anti-inflammatory activities, except for the XOD assay. The dataset highlights the potential dietary benefits of L. pumila leaves as a natural source of antioxidants and anti-inflammatory properties for pharmaceutical and nutraceutical applications.
5-Fluorouracil (5-FU) has been the primary drug used in chemotherapy for colorectal carcinoma, and localizing the drug would be effective in avoiding its side effects and improving therapeutic outcomes. One approach to achieve this is by encapsulating the drug in microbeads. Alginate microbeads, in particular, exhibit promising pH-sensitive properties, making them an attractive option for colon targeting. Thus, the main aim of this study is to formulate and characterize 5-FU-encapsulated alginate microbeads as a pH-sensitive drug delivery system for controlled release in the gastrointestinal tract. In this study, the alginate microbeads encapsulating 5-FU was manufactured using electrospray methods. This method offers the advantages of promoting the formulation of uniformly small-sized microbeads with improved performance in terms of swelling and diffusion rates. The size and shape of the 5-FU microbeads are 394.23 ± 3.077 µm and have a spherical factor of 0.026 ± 0.022, respectively, which are considered acceptable and indicative of a spherical shape. The microbeads' encapsulation efficiency was found to be 69.65 ± 0.18%, which is considered high in comparison to other literature. The attenuated total reflectance - Fourier transform infrared spectroscopy (ATR-FTIR) data confirmed the complexation of sodium alginate with calcium ions, along with the encapsulation of 5-FU in the microbeads matrix. The 5-FU microbeads displayed pH-dependent swelling, exhibiting less swelling in simulated gastric fluid (SGF) than in simulated intestinal fluid (SIF). Additionally, the release of 5-FU from the microbeads is pH-dependent, with the cumulative percentage drug release being higher in simulated intestinal fluid than in SGF. The data indicate that the 5-FU microbeads can be utilized for the delivery of 5-FU in colon-targeted therapy, potentially leading to improved tumor treatment.
Bacillus sp. CCB-MMP212 is a Gram-positive bacterium isolated from mangrove sediment in Matang Perak, Malaysia (4.85496°E, 100.73495°N). Genome sequencing was performed using the Oxford Nanopore and Illumina platforms. The assembled genome was annotated using the rapid annotation subsystem technology server (RAST) (rast.nmpdr.org). The genome size of the Bacillus sp. CCB-MMP212 was 6,151,644 base pairs (bp) with a G+C content of 34.75%. The genome includes 6,311 coding sequences and 58 RNAs. The sequence has been deposited at Genbank with the accession number of JALDQE000000000. Interestingly, an arsenic resistance (ars) operon consisted of arsenic resistance operon repressor (arsR), ACR3 family arsenite efflux transporter (arsB), and arsenate reductase (arsC) genes were found in the genome. In addition, the arsenic inducible gene (arsI), which encoded a dioxygenase with C•As lyase activity, was also found in the ars operon. The enzyme is crucial for the methylation of methylarsonous acid [MAs(III)] and trivalent roxarsone [Rox(III)]. This dataset reveals the genetic ability of this strain in arsenic resistance. To the best of our knowledge, the arsI encoding C•As lyase is rarely reported within the genus Bacillus. Therefore, the dataset presented in this manuscript provides further insight into the arsenic resistance mechanisms of the genus Bacillus.
Bacillus sp. has been reported to be involved in the biodegradation of various hydrocarbon pollutants which can potentially be useful in cleaning up hydrocarbon pollutants. Here we report the draft genome of Bacillus altitudinis strain ST14 isolated from Tunggak river, Gebeng, Kuantan, an area in close proximity to industrial activities. Genome sequencing was conducted using Illumina NovaSEQ 6000 technology. Structural genes in the genome were described, including rRNAs, tRNAs, and ncRNAs. Bacillus altitudinis strain ST14 was sequenced with a length of 3,801,811 bp containing 3,891 coding sequences (CDS). Functional gene annotation reported the presence of six enzymes involved in the degradation of aromatic compounds often found in hydrocarbon pollutants.
Wastewater treatment is a key challenge in the textile industry. The current treatment methods for textile wastewater are insufficient or ineffective for complex dyes generated from the textile industry. This study evaluated the performances of two novel inorganic coagulants with high cationic charges, namely, titanium tetrachloride (TiCl4) and zirconium tetrachloride (ZrCl4). They were utilised to treat textile industry wastewater. Both coagulation processes were performed under the same experimental operational conditions. Turbidity, suspended solids (SS), colour, chemical oxygen demand (COD) and ammonia were measured to assess the efficiencies of the coagulants. Results indicated that ZrCl4 and TiCl4 exhibited high potentials for textile wastewater treatment. ZrCl4 presented high removal efficiency in COD and SS, whereas TiCl4 showed excellent removal in ammonia.
Garcinia mangostana is a tropical fruit plant rich in antioxidant and bears recalcitrant seeds. The extent of water loss and low temperature tolerable by recalcitrant seed varies from regular orthodox seeds. Present study generates transcriptome resources for G. mangostana to postulate potential transcriptome differences between recalcitrant and orthodox seeds during seed germination process. Raw reads of pooled samples used for the assembly have been deposited in genbank accession SRR5412332.
This article investigated how crabs responded to different culture temperatures especially dislocation before molting using a combination of large recording files and computer software. In this novel approach of video recording portunid crab behavioral data, crab culture was recorded at five different acclimation temperatures of 20, 24, 28, 32 and 36 °C. Crabs were reared until the instar stage before being acclimatized for video recording. Large video files (MPEG-TS) were then analyzed using the latest version of Solomon Coder software developed by A. Peter and programmed with Embarcadero® Delphi® XE [1]. Recorded data was analyzed by calculating and marking movements of crabs using the time sequence tool. Additionally, a total movement was counted 30 min before crabs molted from instar stage 8 to instar stage 9. Part of the data is associated with the research article "Thermal tolerance and locomotor activity of blue swimmer crab Portunus pelagicus instar reared at different temperatures" (Azra et al., 2018) [2] and provided here as raw data of Supplementary materials.
This article provides raw datasets of the coral reefs status in Pulau Bidong, southern of South China Sea before and after being strike by the tropical storm Pabuk on January 2019. Data were collected using a rapid coral survey method called Coral Video Transect (CVT) technique. The data were collected along a 100 m transect line set up parallel to the shoreline and at a constant depth. In total, eight transects were surveyed during both periods (pre - August 2016, post - March 2019). Back in laboratory, the footage was then extracted into non-overlapping frames or still images prior to image analysis using Coral Point Count with Excel Extension (CPCe) software. The benthic coral reefs relative percentage cover was automatically generated after the image analysis and represented by five major categories; live coral (C), algae (ALG), other invertebrates (OT), dead coral (DC), and sand silt and rock (SR). Live coral cover was identified up to the genus level. This raw dataset was used in this article. The data provided in this article could be of significant use for future studies especially on coral recovery after the natural disturbances. It can provide a baseline assessment especially for coral reefs management as well as to comprehend changes in coral health status in the face of natural and anthropogenic disturbances. The data presented here support the information in the article Safuan et al. (2020).
White threads fish Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or locally known as bat puntil is a neritic marine organism, and it is widely distributed in Indo Pacific. They serve many important roles in ecosystem services and were discovered to contain many bioactive compounds that are useful for medicinal value. However, despite its abundance in Malaysian seawater, there is still a lack of records on H. leucospilota mitochondrial genome (mitogenome) from Malaysia. The mitogenome of H. leucospilota originating from Sedili Kechil, Kota Tinggi, Johor, Malaysia, is presented here. Whole genome sequencing was successfully sequenced using Illumina NovaSEQ6000 sequencing system and the mitochondrial-derived contigs were assembled using de novo approach. The size of the mitogenome is 15,982 bp which consists of 13 protein-coding genes (PCGs), 21 transfer RNAs, and 2 ribosomal RNAs. The overall composition of nucleotide bases was estimated to be 25.8% for T, 25.9% for C, 31.8% for A and 16.5% for G (with A + T content of 57.6%). Maximum likelihood phylogenetic tree analysis revealed that the mitochondrial Protein-Coding Genes (PCGs) sequence data from our H. leucospilota is closely related to H. leucospilota from accession number MK940237 and H. leucospilota from accession number MN594790, followed by H. leucospilota from accession number MN276190, forming sister group with H. hilla (MN163001), known as Tiger tail sea cucumber. The mitogenome of H. leucospilota will be valuable for genetic research, mitogenome reference and future conservation management of sea cucumber in Malaysia. The mitogenome data of H. leucospilota from Sedili Kechil, Kota Tinggi, Johor, Malaysia is available in the GenBank database repository with accession number ON584426.