Displaying publications 41 - 60 of 54596 in total

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  1. An evaluation of CHD-Specific primer sets for sex typing of birds from feathers
    Ong AH, Vellayan S
    Zoo Biol, 2008 Jan;27(1):62-9.
    PMID: 19360604 DOI: 10.1002/zoo.20163
    The amplification of the highly conserved chromo-helicase-DNA binding region found in both the Z and W chromosome was evaluated with three sets of primers (P8/P2, 1237L/1272H and 2550F/2718R). DNA extracted from feathers through a simple boiling method was used to address its reliability in generating the sex-linked bands. All the bird samples, including the seven bird families that have not been reported previously, were successfully amplified with the primer set 2550F/2718R. The resulting polymerase chain reaction products showed clearly resolved fragments on a conventional agarose gel electrophoresis with size differences ranging from 80 to 540 bp between the two respective ZW gene copies. Although the P8/P2 primer was not as effective under the same conditions, it was able to produce well-resolved Z and W bands from bird species under the Antidea family, whereas the 2250F/2718R primer set only produced a single amplified fragment of a different size between the male and the female. Zoo Biol 27:62-69, 2008. (c) 2007 Wiley-Liss, Inc.
    Matched MeSH terms: Humans
  2. [Development of new nutrient medium for MDCK and Vero cells based on soy hydrolysate obtained using bromeline and assessment of growth characteristics of influenza virus vaccine strains cultivated on them]
    Mazurkova NA, Troshkova, Shishkina LN, Stavskiĭ EA, Drozdov IG
    Zh. Mikrobiol. Epidemiol. Immunobiol., 2011 3 31.
    PMID: 21449080
    AIM: To develop nutrient medium for MDCK and Vero cells based on soy hydrolysate obtained using bromeline and to assess of growth characteristics of influenza virus vaccine strains cultivated on them.

    MATERIALS AND METHODS: Physico-chemical characteristics of hydrolysate were assessed according to FS 42-3874-99. Growth characteristics of nutrient medium based on soy hydrolysate and vaccine strains of influenza virus A/Solomon Islands/03/06 (H1N1), A/Wisconsin/67/2005 (H3N2) and B/Malaysia/2506/2004 were studied on MDCK and Vero cells.

    RESULTS: MDCK and Vero cells grew well on medium based on soy hydrolysate obtained using bromeline with decreased (to 2% and 3% respectively) content of fetal calf serum and allowed effective production of vaccine strains of influenza virus.

    CONCLUSION: Technology for producing of nutrient medium based on hydrolysate of soy flour obtained using bromeline was developed. This medium could successively used for cultivation of continued cell cultures MDCK and Vero used as substrate for tissue culture-based vaccines against influenza.

    Matched MeSH terms: Humans
  3. [Genetic variation of the hemagglutinin and neuraminidase of influenza B viruses isolated in Ningbo during 2010-2012]
    Hu FJ, Li YD, Jiao SL, Zhang S
    Zhonghua Yu Fang Yi Xue Za Zhi, 2013 Dec;47(12):1100-4.
    PMID: 24529267
    To investigate the epidemiological characteristics of influenza B viruses and explore the genetic evolution characteristics of the hemagglutinin(HA) and neuraminidase(NA) genes of local isolated strains in Ningbo, Southeast China, during 2010 to 2012.
    Matched MeSH terms: Humans
  4. [Analysis of genetic features of influenza B virus in Hunan province from 2007 to 2010]
    Liu YZ, Zhao X, Huang YW, Chen Z, Li FC, Gao LD, et al.
    Zhonghua Yu Fang Yi Xue Za Zhi, 2012 Mar;46(3):258-63.
    PMID: 22800599
    To investigate the gene variations of influenza B virus isolated in Hunan province from 2007 to 2010.
    Matched MeSH terms: Humans
  5. [Identification and sequence analysis of E gene of Dengue virus type 2 strain isolated from patient serum in Shenzhen]
    Yang F, He JF, Xian HX, Zhang HL, He YQ, Yang H, et al.
    Zhonghua Yu Fang Yi Xue Za Zhi, 2009 Sep;43(9):798-802.
    PMID: 20137564
    To isolate and identify the pathogen of Dengue fever from Shenzhen city in 2005 - 2006, and to analyze the molecular characteristics of the isolated Dengue virus strain as well as to explore its possible origin.
    Matched MeSH terms: Humans
  6. [Laboratory diagnosis and molecular tracing of dengue bordline cases in Henan Province, 2017]
    Du YH, Li Y, Wang RL, Wang HF, Su J, Xu BL, et al.
    Zhonghua Yu Fang Yi Xue Za Zhi, 2018 Nov 06;52(11):1164-1167.
    PMID: 30419702 DOI: 10.3760/cma.j.issn.0253-9624.2018.11.013
    Objective: To confirm the laboratory diagnosis of dengue bordline cases reported in Henan Province and trace its origin from molecular level in 2017. Methods: The study samples were blood samples (3-5 ml), which came from 8 suspected cases of dengue fever reported in the 2017 direct reporting system of Henan provincial infectious disease monitoring network. Meanwhile, case investigation was conducted according to National dengue fever surveillance programme. Serum were separated from blood samples and tested for Dengue NS1 antigen, IgM & IgG antibodies, and dengue RNA. According to dengue diagnosis criteria, confirmed cases were identified by testing results. Samples carried dengue RNA performed for real-time PCR genotyping and amplification of E gene. Then, the amplicons were sequenced and homological and phylogenetic analyses were constructed. Results: 8 serum samples of suspected dengue cases were collected in Henan Province, 2017. Six of them were diagnosed as dengue confirmed cases. All the dengue confirmed cases belonged to outside imported cases, 5 of them were positive by dengue RNA testing. Genotyping results showed there were 1 DENV1 case, 2 DENV2 cases and 2 DENV3 cases. A DENV2 case and a DENV3 case of this study were traced its origin successfully. The sequence of Pakistan imported DENV2 case belongs to cosmopolitan genotype, which was the most consistent with Pakistan's DENV2 KJ010186 in 2013 (identity 99.0%). The sequence of Malaysia imported DENV3 case belongs to genotype I, which was the most consistent with Singapore's DENV3 KX224276 in 2014(identity 99.0%). Conclusion: The laboratory diagnosis and molecular traceability of dengue cases in Henan Province in 2017 confirmed that all cases were imported and did not cause local epidemics.
    Matched MeSH terms: Humans
  7. [Prevalence of CYP2C19 polymorphisms involved in clopidogrel metabolism in Fujian Han population]
    Wei W, Fang L, Wang N, Zhang T, Zeng JB, Lin MT
    Zhonghua Yi Xue Yi Chuan Xue Za Zhi, 2012 Aug;29(4):420-5.
    PMID: 22875498 DOI: 10.3760/cma.j.issn.1003-9406.2012.04.009
    To investigate the frequency of CYP2C19 polymorphisms involved in clopidogrel metabolism in Fujian Han population.
    Matched MeSH terms: Humans
  8. [Medical exchanges among China and Asiana countries]
    Chen H
    Zhonghua Yi Shi Za Zhi, 1996;26(1):43-9.
    PMID: 11613284
    Among the issues of medical exchanges, medicaments are more often encountered than medical issues, based on ancient Chinese literatures, early in the Han-Jin Dynasties, Thailand, Malaysia, Indonesia, and Brunei had exchanges with China, also in Sui-Tang Dynasties. In the Song-Yuan Dynasties, ancient Singapore and the Philippines also incorporated in exchanges of medicament and goods. In the Ming-Qing dynasties, these 6 Asian countries had even more close contact with China, especially in trades and medical exchanges among the masses, carried out in a large scale thus, promoting the advent and development of TCM in Asiana Regions.
    Matched MeSH terms: Humans
  9. [A perfect pair for the brilliant masterpiece, History of Chinese Medicine-in commemoration of the 130(th) and 140(th) anniversary of the birthday of distinguished modern medical historians, K. Chimin Wong and Wu Lien-teh respectively]
    Zhen Y, Cai JF
    Zhonghua Yi Shi Za Zhi, 2019 Nov 28;49(6):323-329.
    PMID: 32564524 DOI: 10.3760/cma.j.issn.0255-7053.2019.06.001
    It happens that 2019 is the commemoration date for the 130(th) and 140(th) birthday of K. Chimin Wong and Wu Lien-teh respectively, both famous modern Chinese medical historians, the authors of the book History of Chinese Medicine. Originally, they were inhabitants separated thousand miles away, the former in Eastern China, and the other in Penang, Malaysia. Both were busying in their own business works, Wong was specialized in establishing Museum of Medical History, then the first of its kind in China and the world, with splendid results. Whereas, Wu was fighting at the frontier of the overwhelming pneumonic plague in Manchuria, having successfully terminated this virulent infectious disease in a short period of about a quarter, achieving a global reputation in medical world and thus presided the International Plague Conference held in Mukden, China, attended by invited experts from 11 countries. The latter was also active in the creation of hospitals and medical schools, plague prevention and quarantine services in China. Incidentally, when one of them read the book History of Medicine written by the famous American medical historian Fielding Hudson Garrison, to find that this 700+ -page work only includes the contents for Chinese medicine next to nothing and even with wrong descriptions, both were very frustrated and wrote a letter to its author for clarification. They were even more irritated to receive a reply, complaining that the mistakes were not his own, but simply due to shortage of sources, and even that bit of content was from western sources! To wipe up these wrong "foreign descriptions" , they made up their mind to write a similar book of its own in English language, so as to fill up the gaps in this field, hence, the completion of History of Chinese Medicine in a long course of almost 16 years, formally published in 1932, and an enlarged and revised 2nd edition in 1936. This work is divided into 2 books. Book One is devoted to traditional Chines medicine written by K. Chimin Wong; the other Book Two, written by Wu Lien-teh, is devoted to modern and contemporary Chinese medical history, dealing with western medicine to China from its introduction and after experiencing tortuous course and eventually constituting an integral system on biomedicine in China. At the end of the work, there are appendices, including chronological table, geographical names, person names and subject indices. Evaluation of History of Chinese Medicine after 1949 experienced a huge difference. During the first decades, people deemed it to contain lots of mistakes and to have been influenced by national nihilism and western missionary medicine. As a result, the whole work has been roughly translated into a Chinese version, marked by "for criticism" on its cover. After the country carries out a reform and opening to outside world policy, improper appraisal for this work has been changed and is crowned with "brilliant masterpiece" which virtually fills the gaps of the lack of Chinese medical history in western language. It is known that a Chinese version for this work is ongoing and will be officially published soon.
    Matched MeSH terms: Humans
  10. [Progress in the epidemiologic study of Nipah viral encephalitis]
    Lu XF, Wang ZG, Wang BY
    Zhonghua Liu Xing Bing Xue Za Zhi, 2004 Jun;25(6):541-3.
    PMID: 15231143
    Matched MeSH terms: Humans
  11. [Effects of a combined regime of auricular-plaster and body acupuncture in treatment of cervical spondylosis of vertebral artery type]
    Zhang HL, Zhong YM, Peng GM, Wan YG
    Zhongguo Zhen Jiu, 2006 Oct;26(10):697-700.
    PMID: 17117565
    Objective: To study effects of a combined regime of auricular-plaster and body acupuncture in treatment of cervical spondylosis of vertebral artery type and make a preliminary revelation of the mechanism.
    Methods: Ninety-two patients were randomly divided into 2 groups, the treatment group (n = 56) received the combined regime of auricular-plaster and body acupuncture, and the control group (n = 36) received treatment with body acupuncture. Clinical symptoms and signs, therapeutic effect and some indexes about vertebrobasilar hema-kinetics and hema-rheology were investigated before and after treatment.
    Results: The treatment group was better than the control group in the clinical overall effective rate (89.29%) and the clinically control rate (17. 85%), and in improving the following indexes, including dizziness and headache, the vertebrobasilar volume and rate of blood flow etab and IR (P < 0.05).
    Conclusions: A combined regime of auricular-plaster and body acupuncture ameliorates not only main signs but also some indexes about vertebrobasilar hema-kinetics and hema-rheology. This treatment is an effective therapy for cervical spondylosis of vertebral artery type both in Malaysia and in China.
    Matched MeSH terms: Humans
  12. [Su Tian-you's historical relics of practicing medicine and teaching abroad]
    Zhang YS
    Zhongguo Zhen Jiu, 2005 Jun;25(6):443-4.
    PMID: 16309092
    OBJECTIVE: To find historical relics of propagation of Chengjiang acupuncture and moxibustion school of thought abroad in which Cheng Dan' an is representative.

    METHODS: Interview Xing Jingqing, Zhao Zhixing, Qiu Rongqing, students of Ph.D. Su Tianyou in Malaysia, who is the third generation of students of Cheng Dan' an, and collect historical materials about practicing medicine and teaching of Ph.D. Su abroad.

    RESULTS: Su Tianyou is a student of Zeng Tianzhi, a brilliant disciple of Cheng Dan' an. He practiced medicine in 1939 and established Acupuncture and Moxibustion Medical College of Hong Kong in 1940, and he went to 13 countries and districts such as Asia, America and so on for practicing medicine, running schools. He is respectfully called "father of American acupuncture and moxibustion".

    CONCLUSION: Ph.D. Su propagated Chinese medicine abroad, with outstanding achievement in education of medical sciences.
    Matched MeSH terms: Humans
  13. [Diagnosis and Treatment of the First Imported Case of Plasmodium knowlesi Infection in China]
    Pan B, Pei FQ, Ruan CW, Lin RX, Cen YZ, Liu MR, et al.
    Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi, 2016 12;34(6):513-6.
    PMID: 30141606
    Objective: To diagnose and treat the first imported active case of Plasmodium knowlesi infection in China.

    Methods: The clinical information of the patient was collected. Microscopy of blood smear was conducted after Giemsa staining. Genomic DNA was extracted from blood, and PCR was conducted to amplify rDNA. The PCR products were sequenced and analyzed with BLAST

    Results: The patient returned from a one-week tour in a tropical rain forest in Malaysia. The first disease attack occurred in Guangzhou on Oct. 16, 2014, with fever, shivering and sweating. The patient was initially diagnosed as malaria and hospitalized on Oct. 26, 2014. Microscopic observation revealed typical forms of P. knowlesi in blood smear. The red blood cells became enlarged, with big trophozoites appearing as a ring with dual cores and dark brown malaria pigment. The trophozoites were slightly bigger and thicker than P. falciparum. The schizont had 6-8 merozoites, with obvious brown malaria pigment. PCR resulted in a specific band of 1 099 bp. BLAST analysis showed that the sequence of the PCR product was 99% homologous to P. knowlesi (acession No. AM910985.1, L07560.1 and AY580317.1). The patient was diagnosed as P. knowlesi infection, and was then given an 8-day treatment with chloroquine and primaquine, together with dihydroartemisinin piperaquine phosphate tablet. The patient was discharged after recovery on Oct. 28, 2014.

    Conclusion: According to the clinical symptoms, epidemiological history and laboratory test, the patient has been confirmed as P. knowlesi infection. It may also be the first active case of knowlesi malaria reported in China.

    Matched MeSH terms: Humans
  14. [Exploring molecular mechanisms of fucoidan in improving human proximal renal tubular epithelial cells aging by targeting autophagy signaling pathways]
    Fang QJ, Liu JJ, Wan YG, Liu BH, Tu Y, Wu W, et al.
    Zhongguo Zhong Yao Za Zhi, 2020 Dec;45(24):6003-6011.
    PMID: 33496141 DOI: 10.19540/j.cnki.cjcmm.20200709.401
    Fucoidan(FPS) is an effective component of the Chinese patent medicine named Haikun Shenxi, which treats schronic renal failure in clinics, and has the potential anti-aging effects. However, it is still unclear whether FPS can improve renal aging, especially the molecular mechanism of its anti-aging. The human proximal renal tubular epithelial cells(HK-2) in vitro were divided into normal group(N), D-gal model group(D), low dose of FPS group(L-FPS), high dose of FPS group(H-FPS) and vitamin E group(VE), and treated by the different measures, respectively. More specifically, the HK-2 cells in each group were separately treated by 1 mL of 1% fetal bovine serum(FBS) or D-galactose(D-gal, 75 mmol·L~(-1)) or D-gal(75 mmol·L~(-1))+FPS(25 μg·mL~(-1)) or D-gal(75 mmol·L~(-1))+FPS(50 μg·mL~(-1)) or D-gal(75 mmol·L~(-1))+VE(50 μg·mL~(-1)). After the treatment for 24 h, firstly, the effects of D-gal on senescence-associated β-galactosidase(SA-β-gal) staining characteristics and klotho, P53 and P21 protein expression le-vels, as well as adenosine monophosphate activated protein kinase(AMPK)-uncoordinated 51-like kinase 1(ULK1) signaling pathway activation in the HK-2 cells were detected, respectively. Secondly, the effects of FPS and VE on SA-β-gal staining characteristics and klotho, P53 and P21 protein expression levels in the HK-2 cells exposed to D-gal were investigated, respectively. Finally, the effects of FPS and VE on microtubule-associated protein 1 light chain 3(LC3) protein expression level and AMPK-ULK1 signaling pathway activation in the HK-2 cells exposed to D-gal were examined severally. The results indicated that, for the HK-2 cells, the dose of 75 mmol·L~(-1) D-gal could induce the changes of SA-β-gal staining characteristics and klotho, P53 and P21 protein expression levels. That is causing cells aging. FPS and VE could both ameliorate the changes of SA-β-gal staining characteristics and klotho, P53 and P21 protein expression levels in the HK-2 cells exposed to D-gal. That is anti-cells aging, here, the functions of FPS and VE are similar. D-gal could not only induce cell aging but also increase LC3Ⅱ, phosphorylated-AMPK(p-AMPK) and phosphorylated-ULK1(p-ULK1) protein expressions, and activate autophagy-related AMPK-ULK1 signaling pathway. FPS and VE could both improve the changes of LC3Ⅱ, p-AMPK and p-ULK1 protein expression levels in the HK-2 cells exposed to D-gal. That is inhibiting autophagy-related AMPK-ULK1 signaling pathway activation. On the whole, for the human proximal renal tubular epithelial cells aging models induced by D-gal, FPS similar to VE, can ameliorate renal cells aging by possibly inhibiting autophagy-related AMPK-ULK1 signaling pathway activation. This finding provides the preliminary pharmacologic evidences for FPS protecting against renal aging.
    Matched MeSH terms: Humans
  15. [Molecular mechanisms of insulin resistance and interventional effects of Chinese herbal medicine]
    Yee HY, Yang JJ, Wan YG, Chong FL, Wu W, Long Y, et al.
    Zhongguo Zhong Yao Za Zhi, 2019 Apr;44(7):1289-1294.
    PMID: 31090283 DOI: 10.19540/j.cnki.cjcmm.20181105.003
    It is considered that insulin resistance(IR)and its signaling pathway disorder are one of pathogenesis that causes insulin target-organs/issues lesions and their slow progression. The clinical diagnosis index of IR is the homeostatic model of insulin resistance(HOMA-IR)based on fasting blood-glucose and fasting serum insulin. Furthermore, the emerging IR biomarkers including adiponectin may be the references for clinical diagnosis. The influence factors of IR are obesity, chronic microinflammation and a lack of exercise. The major signaling pathways of IR include insulin receptor substrate 1(IRS1)/phosphatidylinositiol-3-kinase(PI3 K)/serine-threonine kinase(Akt)pathway, mitogen-activated protein kinase(MAPK)pathway and Smad3 pathway. In clinics, insulin sensibility and IR could be increased and improved via promoting insulin secretion and enhancing insulin signaling activation. At present, insulin sensitizers treating IR not only have the classic thiazolidinediones and its ramifications but also have the newly discovered metformin and vitamin D. In addition, it is reported that some extracts from single Chinese herbal medicine(CHM)and Chinese herbal compound prescription such as total flavone from the flowers of Abelmoschl manihot, berberine, astragalus polysaccharides and Huang-qi decoction also have the beneficial effects in ameliorating IR. In the field of chronic kidney disease, targeting a common insulin target-organs/issues lesion, the early renal damage in diabetic mellitus, the intervention studies regarding to regulating podocyte IR signaling pathways by CHM will be one of the significant directions in the future.
    Matched MeSH terms: Humans
  16. In vitro demonstration of the invasive ability of Campylobacters
    Tay ST, Devi S, Puthucheary S, Kautner I
    Zentralbl. Bakteriol., 1996 Mar;283(3):306-13.
    PMID: 8861868
    By means of the gentamicin HEp-2 cell invasion assay, it was demonstrated that 82% of the Campylobacters tested were cell-invasive, including 83% of isolates from bloody diarrhoea and 80% of isolates from watery diarrhoea. The large number of invasive strains from watery diarrhoea suggests the possible role of invasiveness in the production of watery diarrhoea. Whether this stage can progress further to more severe symptoms such as bloody diarrhoea remains to be elucidated. Whether this progression to bloody diarrhoea occurs as a result of toxin production is still debatable. In Vero cells, invasion was less efficient and intracellular multiplication was not observed.
    Matched MeSH terms: Humans
  17. [Industrial medicine in India, Nepal, Australia, New Zealand, Indonesia, Malaya and Ceylon]
    BAADER EW
    Zentralbl Arbeitsmed, 1961 Jun;11:141-3 contd.
    PMID: 13685489
    Matched MeSH terms: Humans
  18. [Industrial medicine in India, Nepal, Australia, New Zealand, Indonesia, Malaya and Ceylon. III]
    BAADER EW
    Zentralbl Arbeitsmed, 1961 Aug;11:189-90.
    PMID: 13685488
    Matched MeSH terms: Humans
  19. Survey of paranasal sinuses X-ray. Ethnological clinical analysis of 1,000 Chinese, Indians, Malays, and "others"
    Werner R, Chuah D
    Z Tropenmed Parasitol, 1972 Sep;23(3):316-23.
    PMID: 4634265
    Matched MeSH terms: Humans
  20. [The distribution of Opistorchis and Paragonimus in Malaya and possible sources of infection of man with these parasites]
    Rohde K
    Z Tropenmed Parasitol, 1967 Jul;18(2):152-61.
    PMID: 5628712
    Matched MeSH terms: Humans
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