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Fulltext Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft

Ismail R, Allaudin ZN, Abdullah R, Mohd Lila MA, Nik Abd Rahman NM, Abdul Rahman SO

BMC Cancer, 2016 07 13;16:461.
PMID: 27411985 DOI: 10.1186/s12885-016-2530-8

BACKGROUND: Cancer therapies that kill cancer cells without affecting normal cells is the ultimate mode of treating cancers. The VP3, an avian virus-derived protein, can specifically initiate cell death through several signal transduction pathways leading to apoptosis. In cancer, chemoresistance and cell survivability implicate the cell surface protein, CD147.
METHODS: In this study, transfection of VP3 and silencing of CD147 genes was achieved through the treatment of tumors with pVIVO1-GFP/VP3 (VP3), psiRNA-CD147/2 (shCD147/2), and their combination of CT26 colon cancer cell-induced in mice. The effectiveness of tumor-treatment was ascertained by electrophoresis, TUNEL assay, and flow cytometry analysis. While histopathological and biochemical analysis were used as toxic side effect identification.
RESULTS: The tumor growth delay index (TGDI) after treatment with VP3, shCD147/2, and their combination treatments increased by 1.3-, 1.2-, 2.0- and 2.3-fold respectively, over untreated control. The VP3-shCD147/2 combination treatment was more efficacious then either VP3 or shCD147/2 alone in the retardation of mouse CT26 colorectal cell tumor allograft.
CONCLUSION: The antitumor effect of the combination treatment is the result of synergistic effects of VP3 and shCD147/2 on the tumor cells resulting in apoptosis. Thus, the study shows that combination of VP3 and shCD147/2 treatment can be developed into a potential approach for anticolorectal cancer treatment regimen.
Fulltext Anti-cancer effect of Annona Muricata Linn Leaves Crude Extract (AMCE) on breast cancer cell line

Syed Najmuddin SU, Romli MF, Hamid M, Alitheen NB, Nik Abd Rahman NM

BMC Complement Altern Med, 2016 Aug 24;16(1):311.
PMID: 27558166 DOI: 10.1186/s12906-016-1290-y

Annona muricata Linn which comes from Annonaceae family possesses many therapeutic benefits as reported in previous studies and to no surprise, it has been used in many cultures to treat various ailments including headaches, insomnia, and rheumatism to even treating cancer. However, Annona muricata Linn obtained from different cultivation area does not necessarily offer the same therapeutic effects towards breast cancer (in regards to its bioactive compound production). In this study, anti-proliferative and anti-cancer effects of Annona muricata crude extract (AMCE) on breast cancer cell lines were evaluated.
Current techniques in reprogramming cell potency

Romli F, Alitheen NB, Hamid M, Ismail R, Abd Rahman NM

J Cell Biochem, 2013 Jun;114(6):1230-7.
PMID: 23239017 DOI: 10.1002/jcb.24477

The first successful attempt to reprogram somatic cell into embryonic-like stem cell was achieved on 2006. Since then, it had sparked a race against time to bring this wonderful invention from bench to bedside but it is not easily achieved due to severe problems in term of epigenetic and genomic. With each problem arise, new technique and protocol will be constructed to try to overcome it. This review addresses the various techniques made available to create iPSC with problems hogging down the technique.
Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

Zaidi NE, Shazali NAH, Chor ALT, Osman MA, Ibrahim K, Jaoi-Edward M, et al.

J Vis Exp, 2019 12 14.
PMID: 31885381 DOI: 10.3791/60281

Tumor-associated macrophages (TAMs) have been identified as an important component for tumor growth, invasion, metastasis, and resistance to cancer therapies. However, tumor-associated macrophages can be harmful to the tumor depending on the tumor microenvironment and can reversibly alter their phenotypic characteristics by either antagonizing the cytotoxic activity of immune cells or enhancing anti-tumor response. The molecular actions of macrophages and their interactions with tumor cells (e.g., phagocytosis) have not been extensively studied. Therefore, the interaction between immune cells (M1/M2-subtype TAM) and cancer cells in the tumor microenvironment is now a focus of cancer immunotherapy research. In the present study, a live cell coculture model of induced M1 macrophages and mouse mammary 4T1 carcinoma cells was developed to assess the phagocytic activity of macrophages using a time-lapse video feature using phase-contrast, fluorescent, and differential interference contrast (DIC) microscopy. The present method can observe and document multipoint live-cell imaging of phagocytosis. Phagocytosis of 4T1 cells by M1 macrophages can be observed using fluorescent microscopy before staining 4T1 cells with carboxyfluorescein succinimidyl ester (CFSE). The current publication describes how to coculture macrophages and tumor cells in a single imaging dish, polarize M1 macrophages, and record multipoint events of macrophages engulfing 4T1 cells during 13 h of coculture.
Fulltext Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice

Mohamad NE, Yeap SK, Lim KL, Yusof HM, Beh BK, Tan SW, et al.

Chin Med, 2015;10:3.
PMID: 25699088 DOI: 10.1186/s13020-015-0030-4

Pineapple (Ananas comosus) was demonstrated to be hepatoprotective. This study aims to investigate the reversing effects of pineapple vinegar on paracetamol-induced liver damage in murine model.
Fulltext Nucleating and reinforcing effects of nanobiochar on poly(3-hydroxybutyrate-co-3-hydroxhexanoate) bionanocomposites

Yee Foong Ng L, Ariffin H, Tengku Yasim-Anuar TA, Sakata M, Kawarada T, Yoshimura O, et al.

RSC Adv, 2024 Jul 05;14(30):21971-21981.
PMID: 39006768 DOI: 10.1039/d3ra08721b

This study promotes the use of nanobiochar (NBC) as an environmentally friendly substitute to conventional fillers to improve various properties of biopolymers such as their mechanical strength, thermal stability and crystallization properties. TGA analysis showed a slight increase in onset thermal degradation temperature of the composites by up to 5 °C with the addition of 4 wt% NBC. Non-isothermal DSC analysis determined that the addition of NBC into PHBHHx increases the crystallization temperature and degree of crystallinity of PHBHHx while isothermal DSC analysis demonstrated higher crystallization rate in PHBHHx/NBC composited by up to 54%. PHBHHx incorporated with NBC also exhibited superior tensile strength and modulus versus neat PHBHHx. Increase in mechanical strength was further proven via DMA where PHBHHx/NBC composites maintained higher storage modulus at higher temperatures when compared to neat PHBHHx. PHBHHx/NBC also exhibited no cytotoxicity effect against HaCat cells. This study demonstrates the ability of biochar to act as both nucleating agents and reinforcing agents in biodegradable polymers such as PHBHHx, which could be suitable for packaging application.