MyMedR

Displaying all 3 publications

Abstract:

Sort:

Fulltext Local convexity-preserving C2 rational cubic spline for convex data

Abbas M, Abd Majid A, Ali JM

ScientificWorldJournal, 2014;2014:391568.
PMID: 24757421 DOI: 10.1155/2014/391568

We present the smooth and visually pleasant display of 2D data when it is convex, which is contribution towards the improvements over existing methods. This improvement can be used to get the more accurate results. An attempt has been made in order to develop the local convexity-preserving interpolant for convex data using C(2) rational cubic spline. It involves three families of shape parameters in its representation. Data dependent sufficient constraints are imposed on single shape parameter to conserve the inherited shape feature of data. Remaining two of these shape parameters are used for the modification of convex curve to get a visually pleasing curve according to industrial demand. The scheme is tested through several numerical examples, showing that the scheme is local, computationally economical, and visually pleasing.
TT genotype of the methylenetetrahydrofolate reductase C677T polymorphism is an important determinant for homocysteine levels in multi-ethnic Malaysian ischaemic stroke patients

Mejia Mohamed EH, Tan KS, Ali JM, Mohamed Z

Ann Acad Med Singap, 2011 Apr;40(4):186-91.
PMID: 21678004

INTRODUCTION: The functional point mutation C677T in the methylenetetrahydrofolate reductase (MTHFR) gene, has been reported to contribute to hyperhomocysteinaemia which is a risk factor for atherothrombotic ischaemic strokes. This study evaluated the prevalence of the C677T polymorphism of the gene in Malaysian ischaemic stroke subjects of Malay, Chinese and Indian ethnicities, and its association with homocysteine levels (tHcy).
MATERIALS AND METHODS: A total of 292 subjects were recruited, comprising 150 ischaemic stroke patients and 142 control subjects who were age and sex matched. Plasma homocysteine, serum folate and vitamin B12 were measured in all subjects. Genotyping was carried out using PCR-RFLP.
RESULTS: The homocysteine levels were significantly higher (P = 0.001) in the stroke group (11.35 ± 2.75 μmol/L) compared to the control group (10.38 ± 2.79 μmol/L). The MTHFR C677T genotype distribution for the stroke group was 46%, 40% and 14%, respectively for CC, CT and TT genotypes and 59.9%, 33.8% and 6.3%, respectively for the control group. The genotype and allelic frequencies were significantly different between the 2 groups, with P = 0.02 and P = 0.004 respectively. No significant difference was seen in the genotype distribution inter-ethnically. An increasing tHcy was seen with every additional T allele, and the differences in the tHcy for the different genotypes were significant in both the control (P <0.001) and stroke groups (P <0.001).
CONCLUSION: This study shows that TT genotype of the methylenetetrahydrofolate reductase C677T polymorphic gene is an important determinant for homocysteine levels in Malaysian ischaemic stroke patients.
Gene expression changes in the human fibroblast induced by Centella asiatica triterpenoids

Coldren CD, Hashim P, Ali JM, Oh SK, Sinskey AJ, Rha C

Planta Med, 2003 Aug;69(8):725-32.
PMID: 14531023

The molecular pathways underlying the diverse biological activity of the triterpeniod compounds isolated from the tropical medicinal plant Centella asiatica were studied with gene microarrays and real-time reverse transcription polymerase chain reaction (real-time RT-PCR) to quantify the expression of 1053 human genes in human fibroblasts. Fibroblast cells grown in culture were used as a model system to evaluate the stimulation of wound healing by titrated extract from Centella asiatica (TECA) as well as by the four principal triterpenoid components of Centella. TECA treatment effects the expression of genes involved in angiogenesis and the remodeling of extracellular matrix, as well as diverse growth factor genes. The extent of expression change of TNFAIP6, an extracellular hyaluronan binding protein, was found to be largely dose-dependent, to respond most strongly to the free acids asiatic acid and madecassic acid, and to increase in expression over 48 hours of treatment. These results show that Centella triterpenes evoke a gene-expression response consistent with their prevailing medical uses in the treatment of connective tissue disorders such as wound healing and microangiopathy. The identification of genes modulated by these compounds provides the basis for a molecular understanding of Centella's bioactivity, and opportunities for the quantitative correlation of this activity with clinical effectiveness at a molecular level.

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links