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  1. Fulltext Market surveillance on non-halal additives incorporated in surimi based products using polymerase chain reaction (PCR)–southern hybridization analysis
    Aravindran, S., Sahilah, A.M., Aminah, A.
    International Food Research Journal, 2014;21(6):2095-2099.
    MyJurnal
    In Malaysia, halal certification status for some surimi-based product is still suspicious due to the incorporation of non-halal plasma protein additives as part of the food ingredient. This study was conducted to determine the presence of plasma protein additives that have been incorporated into surimi-based product using Polymerase Chain Reaction (PCR)-Southern Hybridization method which able to differentiate 7 type (beef, chicken, duck, goat, buffalo, lamb and pork) of species on a single chip. A random of 17 (n = 17*3) different brands of surimi-based product was purchased from Selangor local market in January 2013. Of 17 brands, 3 (n = 3*3) brands were positive for chicken DNA and 1 (n = 1*3) brand was positive for goat DNA, while remainder 13 brands (n = 13*3) has no DNA species detected. In presence study, it is evidence that PCR-Southern Hybridization analysis offered a reliable result due to its highly specific and sensitive properties in detecting plasma protein incorporation in surimi-based product.
  2. Fulltext Halal authentication in Malaysia context: potential adulteration of non-Halal ingredients in meatballs and surimi products
    Sahilah, A.M., Laila Liyana, M.N., Aravindran, S., Aminah, A., Mohd Khan, A.
    International Food Research Journal, 2016;23(5):1832-1838.
    MyJurnal
    Halal is a term that describes substances that are deemed ‘pure and clean’ which Muslims are
    allowed to consume according to Islamic law. The industrialization of food processing in the
    20th and 21st centuries has exposed Muslims community to various ingredients such as blood
    plasma, transglutaminase and gelatin introduced in meatballs and surimi product. Muslims
    are facing difficulties to ascertain which products are permitted or not under the Islamic law.
    Thus, this paper is to give knowledge of non-halal ingredients being introduced in meatballs
    and surimi products for consumers, researchers and policy makers. Local halal logo issued by
    Department of Islamic Development Malaysia (JAKIM) is needed to imply that all ingredients
    used in the food production and processing are Syariah compliance. The scientific evidence
    to substantiate any claim on Halal issue was developed based on several methods including
    PCR-based methods with different mitochondria and chromosomal DNA (MtDNA and cDNA)
    primers, real-time PCR with different probes and DNA binding agent, loop-mediated isothermal
    amplification (LAMP) with different primers developed, PCR- RFLP, ELISA and etc.
  3. Fulltext In vitro antioxidant activity and hepatoprotective effects of Lentinula edodes against paracetamol-induced hepatotoxicity
    Sasidharan S, Aravindran S, Latha LY, Vijenthi R, Saravanan D, Amutha S
    Molecules, 2010 Jun 23;15(6):4478-89.
    PMID: 20657455 DOI: 10.3390/molecules15064478
    BACKGROUND: The objective of this study was to investigate the antioxidant and hepatoprotective effects of methanolic extracts of L. edodes and the determination of their total phenolics content.

    RESULTS: The amount of total phenolics was estimated to be 70.83 mg Gallic Acid Equivalent (GAE) per gram of dry extract. The antioxidant activity of the L. edodes extract was 39.0% at a concentration of 1 mg/mL and was also concentration dependant, with an EC(50) value of 4.4 mg/mL. Different groups of animals (Wister albino mice) were administered paracetamol (1 g/kg, p.o.). L. edodes extract at a dose of 200 mg/kg was administered to the paracetamol treated mice for seven days. The effects of L. edodes extract on serum transaminases (SGOT, SGPT), alkaline phosphatase (ALP) and bilirubin were measured in the paracetamol-induced hepatotoxic mice. L. edodes extract produced significant (p < 0.05) hepatoprotective effects by decreasing the activity of serum enzymes and bilirubin.

    CONCLUSIONS: From these results, it was suggested that L. edodes extract could perhaps protect liver cells from paracetamol-induced liver damage by its antioxidative effect on hepatocytes, hence diminishing or eliminating the harmful effects of toxic metabolites of paracetamol.

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