A dietary study was conducted to assess the use of mushroom stalk (MM), Pleurotus sajor caju, an agriculture waste and
soy bean meal (SBM) as partial and complete replacement of fishmeal (FM) in tilapia fingerling diets. The study was
conducted for 56 days. The diets were processed into sinking pellets. The experimental diets were formulated to replace
fish meal protein at 0, 33, 67 and 100% (diet 4, 3, 2 and 1). In this experiment, Nile tilapia fingerlings weighing about
1.00 g were fed each of four isonitrogenous diet. They were randomly distributed into 12 plastic tanks with each tank
stocking 20 fish. The experiment was conducted in three replicates for each treatment. All the diets were well accepted
by the fish. No physical abnormalities were observed in all treatments. Good growth performance was shown in 33%
replacement (diet 3) of FM diet. However, diet 1 (100% replacement) gave the highest increment in crude protein level
and the best result of feed conversion ratio (FCR) and protein efficiency ratio (PER). Furthermore, the 100% replacement
of FM (diet 1) was the best with a production cost of RM2.61/kg.
Microalgae play an important function in aquatic environments by serving as the energy foundation of the food chain for all aquatic species. Microalgae produce a wide range of bioproducts, including polysaccharides, lipids, pigments, proteins, vitamins, bioactive chemicals, and antioxidants. In this study, a dataset of identification of three microalgae isolated from freshwater riverine areas at Glami Lemi River, Malaysia is presented. The identification data was acquired using molecular identification using the PCR method and morphological observation. The morphological observation of the microalgae isolates, GL01, GL02, and GL03, showed the closest features and characteristics with Chlorella sp., Ankinstrodesmus sp., and Tetradesmus (Scenedesmus) sp., respectively. The sequence similarity analysis of partial 18s rDNA gene using BLASTn identified the three microalgae species as Chlorella sorokiniana for isolate GL01, Ankistrodesmus fusiformis for isolate GL02, and Tetradesmus obliquus for isolate GL03. Both morphological observation and molecular identification were in agreement in the determination of the species. This is the first report on the three microalgae species isolated from this area.