This study examines the antioxidant capacity of linusorb (LOs) and their interaction with phospholipids (PLs) in hot-pressed flaxseed oil (HO), which is valued for its enhanced oxidative stability in Northwest China. Utilizing antioxidant assays and molecular simulations, the research aims to: 1) Assess oxidation indices to identify optimal roasting conditions and monitor LOs during the oxidation of HO, triacylglycerol (TAG), and TAG + LOs; 2) Analyze the oxidation kinetics of crude LOs (cLOs) from cold-pressed oil (CO) and LOs (hLOs) from HO in the presence of PLs; and 3) Molecular dynamics simulations to explore the interactions between PLs and LOs. The findings indicate that HO exhibits superior oxidative stability due to a higher concentration of LOs and PLs, with altered the antioxidative behavior of LOs. Simulation suggests that PLs can bind to LOs, facilitating their migration to the water-oil interface and enhancing their antioxidant effects.