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Fulltext Comparison of three different methods in the assessment of neutrophil function

Noah RM, Jais MR, Noh LM

Med J Malaysia, 1995 Jun;50(2):136-40.
PMID: 7565182

Three different methods to measure the oxidative respiratory burst of neutrophils were performed. Of the three, the chemiluminescence technique was observed to be the most sensitive among them. The strong statistical correlation and an acceptable agreement between chemiluminescence with that of the killing assay provides evidence for using the chemiluminescence assay as an alternative method of detecting gross defects of neutrophil respiratory burst killing assays.
Evaluation of variable parameters in neutrophil function test using chemiluminescence assay

Noah RM, Jais MR, Noh LM

Malays J Pathol, 1994 Dec;16(2):157-60.
PMID: 9053565

Variable parameters in chemiluminescence assay, one of the methods used to assess the functional capacity of neutrophils, were evaluated for suitable adaptation locally. The use of pooled normal human serum as compared to single normal human serum in opsonizing particles for phagocytosis was found to exhibit lower chemiluminescence activity (reduction range of 30%-50%). A similar degree of depression was observed when the particles were opsonized using normal human serum in comparison to that using autologous serum. Different intensity of chemiluminescence was also noted when the opsonized particle used was the Oxford strain of Staphylococcus aureus (NCTC 6571) in contrast to a strain of Staphylococcus aureus isolated from a patient. The results obtained warrant clinicians to deliver appropriate samples as best they can when the chemiluminescence assay is requested.
Fulltext Analgesic Properties of Nigella Sativa and Eucheuma Cottonii Extracts

Zakaria A, Jais MR, Ishak R

J Nat Sci Biol Med, 2018;9(1):23-26.
PMID: 29456388 DOI: 10.4103/jnsbm.JNSBM_131_17

BACKGROUND: This study investigates the analgesic properties of the aqueous extracts of Nigella sativa and Eucheuma cottonii in mice. The analgesic properties of both extracts were evaluated in an experimental model of acetic acid-induced writhing test.
MATERIALS AND METHODS: The mice were divided into four different groups and received the test extracts and the standard drug (aspirin) for 14 days via force-feeding. On day 15, the mice were injected with 5% acetic acid, and the number of abdominal constriction and elongation of hind limb (writhes) were counted for 20 min.
RESULTS: The numbers of writhes were counted starting after 5 min of the acetic acid injection. The N. sativa extracts significantly reduced the number of writhes as compared to the control group. Both of the extracts revealed a comparable result as referred to the aspirin effects in the mice.
CONCLUSIONS: These findings indicate that N. sativa and E. cottonii may possess protective active constituent that is effective in reducing the sensation of pain in mice.

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