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  1. Fulltext Validation of tissue microarray for immunohistochemical analysis of oral squamous cell carcinoma by using virtual cores
    A, Ramanathan, RM, Chong, ZW, Tay, C, Siow-Wee, TG, Kallarakkal, Kassim NLA
    Ann Dent, 2014;21(1):16-23.
    MyJurnal
    There is significant amount of research done
    on Oral Squamous cell carcinoma (OSCC). One research
    technique is immunohistochemical (IHC) analysis using
    whole sections. With little availability of OSCC tissues
    high throughput analysis such as Tissue Microarray
    (TMA) are capable of efficient analysis of small samples.
    However, the results become questionable if the tumor
    exhibits high degree of heterogeneity as TMA cores might
    not accurately represent the whole section. Aim: The aim
    of this study is to determine the optimal number of TMA
    cores required to provide an accurate representation of
    the whole section with IHC analysis in OSCC. Materials
    and Methods: Twenty tissue samples stained with
    anti-p53 antibody were scanned at 40x magnification.
    Three to six virtual cores of size 0.6 mm, 1.0 mm and
    1.5 mm were drawn on the scanned slides. H-scores
    were obtained for both whole sections and cores using
    NuclearQuant (3DHistech, Budapest, Hungary) software
    after eliminating non-tumour cells and artifacts manually.
    The correspondence between the cores and whole sections
    were calculated using intra-class correlation and one
    sample t-test. Results: Good correlation was obtained
    with just a single core of 0.6mm (0.826). Subsequent
    increase in core number and size resulted in improved
    correlation coefficient and smaller confidence interval.
    Conclusion: Three TMA cores of 0.6 mm would be the
    most optimal, as not only was there very strong correlation
    with the whole tissue section, the extra core will also be
    able to act as confirmation if the results of the first 2 cores
    are in doubt.
  2. Fulltext Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
    Haque N, Kasim NHA, Kassim NLA, Rahman MT
    Cell Prolif, 2017 Aug;50(4).
    PMID: 28682474 DOI: 10.1111/cpr.12354
    OBJECTIVES: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome.

    MATERIALS AND METHODS: The PBMC (n = 7) were cultured either in RPMI-1640 containing L-glutamine and 50 units/ml Penicillin-Streptomycin (BM) or in BM with either AuHS or FBS. Viability, proliferation and differentiation of PBMC were evaluated. Paracrine factors present in the secretomes (n = 6) were analysed using ProcartaPlex Human Cytokine panel (17 plex). Ingenuity Pathway Analysis (IPA) was performed to predict activation or inhibition of biological functions related to tissue regeneration.

    RESULTS: The viability of PBMC that were cultured with FBS supplement was significantly reduced at 96 h compared to those at 0 and 24 h (P 

  3. Fulltext Antibodies against citrullinated proteins in relation to periodontitis with or without rheumatoid arthritis: a cross-sectional study
    Lew PH, Rahman MT, Safii SH, Baharuddin NA, Bartold PM, Sockalingam S, et al.
    BMC Oral Health, 2021 07 20;21(1):360.
    PMID: 34284769 DOI: 10.1186/s12903-021-01712-y
    BACKGROUND: Previous studies have reported conflicting findings between serum anti-citrullinated protein antibodies (ACPA) levels in rheumatoid arthritis (RA) participants with and without periodontitis (Pd). This study aimed to analyse possible correlations between serum ACPA levels and clinical parameters in Pd and RA participants.

    METHODS: Full mouth periodontal examination (probing pocket depth, clinical attachment levels, gingival bleeding index, visual plaque index) was conducted and serum samples obtained from 80 participants comprising RA, Pd, both RA and Pd (RAPd) and healthy individuals (HC). Erythrocyte sedimentation rates (ESR) and periodontal inflamed surface area (PISA) were obtained. Serum samples were analysed for ACPA quantification using enzyme-linked immunosorbent assay (ELISA).

    RESULTS: Median levels (IU/mL) of ACPA (interquartile range, IQR) in RAPd, RA, Pd and HC groups were 118.58(274.51), 102.02(252.89), 78.48(132.6) and 51.67(91.31) respectively. ACPA levels were significantly higher in RAPd and RA as compared to HC group (p  RA > Pd > HC. However, lack of any significant correlation between the serum ACPA levels with the clinical Pd and RA parameters warrants further studies to investigate the causal link between RA and Pd for such a trend. Further studies involving more inflammatory biomarkers might be useful to establish the causal link between Pd in the development and progression of RA or vice versa.

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