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Fulltext The Chinese version of the physical activity questionnaire for adolescents: a psychometric validity, reliability, and invariance study

Qin L, Ho WKY, Khoo S

BMC Public Health, 2024 Nov 11;24(1):3117.
PMID: 39529046 DOI: 10.1186/s12889-024-20563-0

BACKGROUND: The Physical Activity Questionnaire for Adolescents (PAQ-A) is one of the most commonly used questionnaires for assessing adolescents' levels of moderate to vigorous physical activity (PA). Although a Chinese version of the PAQ-A (comprising nine items) exists, it has been assessed for only internal consistency coefficients, test-retest reliability, and correlations with the GT3X + accelerometer. Therefore, this study aimed to evaluate the psychometric properties of the Chinese version of the PAQ-A among Chinese adolescents.
METHODS: This study included three samples of 1,101 secondary school students (12-19 years old). The first sample consisted of 518 participants (50.4% males, 49.6% females) for correlation tests and exploratory factor analysis (EFA). The second sample consisted of 227 participants (55.75% males, 44.24% females) for confirmatory factor analysis (CFA), convergent validity, and sample size invariance tests. The third sample consisted of 356 participants (54.50% male, 45.50% female) for the internal consistency reliability test, invariance test (cross sample size, education level and gender), and t-test.
RESULTS: Pearson correlation analysis of the PAQ-A indicated that all the total-item correlations exceeded 0.2, indicating good consistency across the items. Subsequent EFA of the Chinese version of the PAQ-A revealed a two-factor structure. CFA subsequently validated this structure. One of the items exhibited a standardized loading below 0.4 and was excluded. The exclusion of this item resulted in increased standardized loadings for the remaining items, ranging from 0.40 to 0.82, which indicates improved fit indices. This adjustment underscores the questionnaire's satisfactory convergent validity and robust discriminant validity. The overall Cronbach's alpha was 0.821, with the values for the first and second factors being 0.757 and 0.716, respectively. The questionnaire demonstrated stable invariance across sample sizes and education levels and additionally showed partial scalar invariance across genders. A t-test revealed a significant difference between males and females, which aligns with previous findings. These findings supported the construct validity of the questionnaire.
CONCLUSION: This study validated the Chinese version of the PAQ-A for assessing adolescent PA in China, with a two-factor structure improved by removing one item.
Fulltext Factor structure and measurement invariance of exercise self-efficacy scale among secondary school students in China

Qin L, Ho WKY, Khoo S

Sci Rep, 2025 Feb 09;15(1):4844.
PMID: 39924573 DOI: 10.1038/s41598-025-89287-4

Exercise Self-Efficacy Scale (ESES) is widely used to assess individuals' exercise self-efficacy through self-reporting. It includes one factor and 18 items that gauge one's confidence in exercising under various conditions, such as when tired, stressed, or in unfavourable weather. Evidence indicates that differences in the original factor structure and psychometric properties were observed across different populations, including school-age students, university students, and adults. This study examined the factor structure, reliability, convergent validity, and measurement invariance of the Chinese version of the ESES among secondary school students. Data from 856 students (age: M = 13.80, SD = 0.94) were analysed using exploratory and confirmatory factor analyses to identify the best-fitting factor structure. The reliability and convergent validity were tested using the collected data. Configural, metric, and scalar invariances, as well as the likelihood ratio test, were tested for measurement invariance. A 14-item, two-factor structure of the ESES, consistently demonstrated the best fit among secondary school students. The two-factor structure showed strong internal consistency reliability (McDonald's Omega of 0.921 and 0.843) and satisfactory convergent validity (average variance extracted values of 0.582 and 0.478, composite reliability values of 0.917 and 0.845). Multi-group confirmatory factor analysis (likelihood ratio test, p > 0.01) revealed scalar measurement invariance across sex, ethnic backgrounds, grades, and school locations. These findings suggest that the 14-item, two-factor Chinese version of the ESES is suitable for use with secondary-school students. Future studies could confirm these findings by examining the 14-item, two-factor ESES in diverse samples, considering ethnicity, socioeconomic status, and age range.
Fulltext Development and validation of a measurement instrument for student assessment of quality physical education in Chinese secondary schools

Qin L, Ho WKY, Khoo S

PLoS One, 2025;20(6):e0324227.
PMID: 40471878 DOI: 10.1371/journal.pone.0324227

There is a growing emphasis on developing Quality Physical Education (QPE) programs. However, a research gap exists due to the lack of measurement instruments to assess QPE for students. This study aimed to develop an instrument to assess QPE implementation and its validity and reliability in China. This study comprised three phases. In Phase 1, a conceptual framework for QPE was established using grounded theory based on interviews with 22 PE teachers and 20 students. In Phase 2, using the conceptual framework from Phase 1, we developed an item pool supported by a literature review, expert evaluations, and student interviews. In Phase 3, a cross-sectional study was conducted with secondary school students (705 participants) to analyse the items and assess the instrument's reliability and validity through exploratory and confirmatory factor analysis, as well as test-retest analysis. The final 45-item instrument, comprising four subscales (student, family, school, and community) across 10 factors, demonstrated strong validity and reliability. Model fit indices met established thresholds (e.g., CFI and TLI ≥ .90, as well as RMSEA ≤ .08). The composite reliability and average variance extracted values for each factor exceeded.7 and.5, respectively, with the test-retest reliability also exceeding.7, indicating high reliability and validity. This study addresses a critical methodological gap in QPE research by developing a culturally contextualized assessment instrument that explicitly identifies and measures students' perceptions of QPE implementation. This instrument enables systematic monitoring of QPE practices from students' perspectives, informing evidence-based policymaking and resource allocation. Integrating student, family, school, and community subscales supports holistic interventions to improve the quality of PE.
Fulltext Physical demands and physiological response of soccer referees in high-level matches: A systematic review

Zhang L, Geok SK, Wazir MRWN, Qin L

PLoS One, 2025;20(1):e0315403.
PMID: 39854410 DOI: 10.1371/journal.pone.0315403

BACKGROUND: The match physical demands placed on soccer referees are intrinsically connected to their capacity to make accurate judgments, becoming the second most studied theme in associate soccer refereeing.
OBJECTIVE: This study aims to review the external and internal load performed by soccer referees in high-level competitions, to identify changes in these indicators over different periods as the competition progresses, and to analyze the standards for dividing speed zones and heart rate zones.
METHODS: Web of Science, PubMed, Scopus, and EBSCOhost were thoroughly searched. Grey literature sources and Google Scholar were also consulted, with a focus on analysing and comparing the physical demands of soccer referees at different phases of high-level matches.
RESULTS: A total of 14 manuscripts were included in this review. Studies revealed that the total distance (TD) covered by referees during a full match ranged from 9 to 12 km. High-intensity running (HIR) constituted 2.0-18.7% of TD, accounting for approximately 38% of total time (TT). Referees reached 80-100% of their maximal heart rate during matches. The standards for dividing speed zones and heart rate zones varied among the selected studies.
DISCUSSION: This systematic review aimed to provide a comprehensive overview of referees' physical demands (e.g., TD, HIR, and HR) to offer practitioners valuable biological data for training and competition preparation. The lack of uniform criteria for dividing speed and heart rate zones limits data collection, thereby affecting the reporting of distances covered at different exercise intensities.
Crystal structures of Nipah and Hendra virus fusion core proteins

Lou Z, Xu Y, Xiang K, Su N, Qin L, Li X, et al.

FEBS J, 2006 Oct;273(19):4538-47.
PMID: 16972940

The Nipah and Hendra viruses are highly pathogenic paramyxoviruses that recently emerged from flying foxes to cause serious disease outbreaks in humans and livestock in Australia, Malaysia, Singapore and Bangladesh. Their unique genetic constitution, high virulence and wide host range set them apart from other paramyxoviruses. These characteristics have led to their classification into the new genus Henpavirus within the family Paramyxoviridae and to their designation as Biosafety Level 4 pathogens. The fusion protein, an enveloped glycoprotein essential for viral entry, belongs to the family of class I fusion proteins and is characterized by the presence of two heptad repeat (HR) regions, HR1 and HR2. These two regions associate to form a fusion-active hairpin conformation that juxtaposes the viral and cellular membranes to facilitate membrane fusion and enable subsequent viral entry. The Hendra and Nipah virus fusion core proteins were crystallized and their structures determined to 2.2 A resolution. The Nipah and Hendra fusion core structures are six-helix bundles with three HR2 helices packed against the hydrophobic grooves on the surface of a central coiled coil formed by three parallel HR1 helices in an oblique antiparallel manner. Because of the high level of conservation in core regions, it is proposed that the Nipah and Hendra virus fusion cores can provide a model for membrane fusion in all paramyxoviruses. The relatively deep grooves on the surface of the central coiled coil represent a good target site for drug discovery strategies aimed at inhibiting viral entry by blocking hairpin formation.
Comparison of virus-capsid mimicking biologic-shell based versus polymeric-shell nanoparticles for enhanced oral insulin delivery

Cui Z, Cui S, Qin L, An Y, Zhang X, Guan J, et al.

Asian J Pharm Sci, 2023 Sep;18(5):100848.
PMID: 37881796 DOI: 10.1016/j.ajps.2023.100848

Virus-capsid mimicking mucus-permeable nanoparticles are promising oral insulin carriers which surmount intestinal mucus barrier. However, the impact of different virus-capsid mimicking structure remains unexplored. In this study, utilizing biotin grafted chitosan as the main skeleton, virus-mimicking nanoparticles endowed with biologic-shell (streptavidin coverage) and polymeric-shell (hyaluronic acid/alginate coating) were designed with insulin as a model drug by self-assembly processes. It was demonstrated that biologic-shell mimicking nanoparticles exhibited a higher intestinal trans-mucus (>80%, 10 min) and transmucosal penetration efficiency (1.6-2.2-fold improvement) than polymeric-shell counterparts. Uptake mechanism studies revealed caveolae-mediated endocytosis was responsible for the absorption of biologic-shell mimicking nanoparticles whereas polymeric-shell mimicking nanoparticles were characterized by clathrin-mediated pathway with anticipated lysosomal insulin digestion. Further, in vivo hypoglycemic study indicated that the improved effect of regulating blood sugar levels was virus-capsid structure dependent out of which biologic-shell mimicking nanoparticles presented the best performance (5.1%). Although the findings of this study are encouraging, much more work is required to meet the standards of clinical translation. Taken together, we highlight the external structural dependence of virus-capsid mimicking nanoparticles on the muco-penetrating and uptake mechanism of enterocytes that in turn affecting their in vivo absorption, which should be pondered when engineering virus-mimicking nanoparticles for oral insulin delivery.
Mental Health, Loneliness, and Social Support Among Undergraduate Students: A Multinational Study in Asia

Backhaus I, Fitri M, Esfahani M, Ngo HT, Lin LJ, Yamanaka A, et al.

Asia Pac J Public Health, 2023 May;35(4):244-250.
PMID: 37226778 DOI: 10.1177/10105395231172311

In this study, we aimed to investigate the prevalence of poor mental health and its association with loneliness and social support among 3531 undergraduate students in nine Asian countries. Mental health was assessed using the Self-Reporting Questionnaire, which was developed by the World Health Organization. Across the entire sample, we detected that nearly half of the students reported poor mental health according to the Self-Reporting Questionnaire and nearly one out of seven students felt lonely. While feeling lonely increased the odds of experiencing poor mental health (odds ratio [OR]), moderate (OR: 0.35) and strong social support (OR: 0.18) decreases the odds of experiencing poor mental health. The high prevalence of poor mental health calls for further in-depth investigations and implementation of mental health support interventions.
Fulltext PDLLA-Zn-nitrided Fe bioresorbable scaffold with 53-μm-thick metallic struts and tunable multistage biodegradation function

Shen D, Qi H, Lin W, Zhang W, Bian D, Shi X, et al.

Sci Adv, 2021 Jun;7(23).
PMID: 34088662 DOI: 10.1126/sciadv.abf0614

Balancing the biodegradability and mechanical integrity of a bioresorbable scaffold (BRS) with time after implantation to match the remodeling of the scaffolded blood vessel is important, but a key challenge in doing so remains. This study presents a novel intercalated structure of a metallic BRS by introducing a nanoscale Zn sacrificial layer between the nitrided Fe platform and the sirolimus-carrying poly(d,l-lactide) drug coating. The PDLLA-Zn-FeN BRS shows a multistage biodegradation behavior, maintaining mechanical integrity at the initial stage and exhibiting accelerated biodegradation at the subsequent stage in both rabbit abdominal aortas and human coronary arteries, where complete biodegradation was observed about 2 years after implantation. The presence of the nanoscale Zn sacrificial layer with an adjustable thickness also contributes to the tunable biodegradation of BRS and allows the reduction of the metallic strut thickness to 53 μm, with radial strength as strong as that of the current permanent drug-eluting stents.