A large number of micro-organisms colonize in human body. These resident microbes gives protection to the healthy human body, but at the same time when the immune system of an individual is weakened these microbes can become pathogenic and can cause infection. These microbes are adapting to the human environment very fast and using various mechanisms for survival and pathogenicity. Recently several cases has been reported of multi drug resistant Staphylococcus aureus especially in hospital settings. In this connection, present paper discusses the assessment of antimicrobial activity of isolated methicillin resistant Staphylococcus aureus strain (MRSA-MSU-101) and confirms the tetracyclin sensitivity against the isolated strain. Initially, isolation of Staphylococcus aureus strains from skin of various community people has been carried out, then the screening has been done for the methicillin resistant Staphylococcus aureus strain. One strain shown methicillin resistance labelled as MRSA-MSU-101 and considered as potentially pathogenic. Subsequently, for this strain tetracyclin efficiency assessed by antibiotic disc diffusion test (Kirby-Bauer method). This strain MRSA-MSU-101 has shown significant susceptibility against tetracyclin (1cm-diameter, zone of inhibition). Therefore, tetracyclin antibiotic is recommended for treatment against methicillin resistant Staphylococcus aureus strains.
Background: A large number of undiscovered fungal species still exist on earth, which can be useful for bioprospecting, particularly for single cell oil (SCO) production. Mortierella is one of the significant genera in this field and contains about hundred species. Moreover, M. alpina is the main single cell oil producer at commercial scale under this genus. Methods: Soil samples from four unique locations of North-East Libya were collected for the isolation of oleaginous Mortierella alpina strains by a serial dilution method. Morphological identification was carried out using light microscopy (Olympus, Japan) and genetic diversity of the isolated Mortierella alpina strains was assessed using conserved internal transcribed spacer (ITS) gene sequences available on the NCBI GenBank database for the confirmation of novelty. The nucleotide sequences reported in this study have been deposited at GenBank (accession no. MZ298831:MZ298835). The MultAlin program was used to align the sequences of closely related strains. The DNA sequences were analyzed for phylogenetic relationships by molecular evolutionary genetic analysis using MEGA X software consisting of Clustal_X v.2.1 for multiple sequence alignment. The neighbour-joining tree was constructed using the Kimura 2-parameter substitution model. Results: The present research study confirms four oleaginous fungal isolates from Libyan soil. These isolates (barcoded as MSU-101, MSU-201, MSU-401 and MSU-501) were discovered and reported for the first time from diverse soil samples of district Aljabal Al-Akhdar in North-East Libya and fall in the class: Zygomycetes; order: Mortierellales. Conclusions: Four oleaginous fungal isolates barcoded as MSU-101, MSU-201, MSU-401 and MSU-501 were identified and confirmed by morphological and molecular analysis. These fungal isolates showed highest similarity with Mortierella alpina species and can be potentialistic single cell oil producers. Thus, the present research study provides insight to the unseen fungal diversity and contributes to more comprehensive Mortierella alpina reference collections worldwide.