Although recent research looked at the unified theory of acceptance and use of technology (UTAUT) model among academicians, there is still lacking an adequate account for their technology adoption intentions and behaviors in the face of the COVID-19 virus. This study applies the conservation of resource (COR) theory to ground the statistical results investigating the moderation and moderated mediation effects of COVID-19 anxiety in relation to the UTAUT model. According to the COR theory, anxiety linked with the COVID-19 pandemic endangers resources such as human connections (death of friends and relatives), job security and finances, as well as physical health and psychological well-being. Since resource loss is more salient according to COR theory, academicians are more willing to employ technologies like Zoom, MS Teams, and Google Classroom since the COVID-19 threat is existential and the resource loss is eminent. To test the theory, data was collected through an anonymous survey during the months of December 2020 and January 2021. The resultant data from 215 academicians was analyzed using Hayes Process Macro in SPSS. COVID-19 Anxiety positive moderates both the direct effects of Performance Expectance-Behavior Intention and the mediating Performance Expectance-Behavior Intension-Usage relationships. Similar results were seen for other predictors, albeit the moderated mediations for Effort Expectancy and Facilitating Conditions were not significant. These findings overwhelmingly corroborate the claims that COR theory more comprehensively explains the effects of COVID-19 anxiety among academicians in relation to the UTAUT model.
The application of antibodies in enzyme-linked immunosorbent assay (ELISA) is the basis of this diagnostic technique which is designed to detect a potpourri of complex target molecules such as cell surface antigens, allergens, and food contaminants. However, development of the systematic evolution of Ligands by Exponential Enrichment (SELEX) method, which can generate a nucleic acid-based probe (aptamer) that possess numerous advantages compared to antibodies, offers the possibility of using aptamers as an alternative molecular recognition element in ELISA. Compared to antibodies, aptamers are smaller in size, can be easily modified, are cheaper to produce, and can be generated against a wide array of target molecules. The application of aptamers in ELISA gives rise to an ELISA-derived assay called enzyme-linked apta-sorbent assay (ELASA). As with the ELISA method, ELASA can be used in several different configurations, including direct, indirect, and sandwich assays. This review provides an overview of the strategies involved in aptamer-based ELASA.
Dengue fever is endemic in Malaysia, contributing to significant economic and health burden in the country. Aedes aegypti and Ae. albopictus are the main vectors of the dengue virus (DENV), which circulates in sylvatic and human transmission cycles and has been present in Malaysia for decades. The study investigated the presence and distribution of DENV in urban localities in the Klang Valley, Peninsular Malaysia. A total of 364 Ae. aegypti and 1,025 Ae. albopictus larvae, and 10 Ae. aegypti and 42 Ae. albopictus adult mosquitoes were screened for the presence of DENV. In total, 31 (2.2%) samples were positive, of which 2 Ae. albopictus larvae were co-infected with two serotypes, one with DENV-2 and DENV-3 and the other with DENV-3 and DENV-4. Phylogenetic analysis determined that the isolates belonged to DENV-1 genotype I (1 Ae. aegypti adult), DENV-2 (1 Ae. albopictus larva), DENV-3 genotype V (3 Ae. aegypti larvae and 10 Ae. albopictus larvae) and DENV-4 genotype IV (6 Ae. aegypti larvae and 12 Ae. albopictus larvae), a sylvatic strain of DENV-4 which was most closely related with sylvatic strains isolated from arboreal mosquitoes and sentinel monkeys in Peninsular Malaysia in the 1970s. All four DENV serotypes were co-circulating throughout the study period. The detection of a sylvatic strain of DENV-4 in Ae. aegypti and Ae. albopictus mosquitoes in urban areas in Peninsular Malaysia highlights the susceptibility of these vectors to infection with sylvatic DENV. The infectivity and vector competence of these urban mosquitoes to this strain of the virus needs further investigation, as well as the possibility of the emergence of sylvatic virus into the human transmission cycle.