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Nilai taksonomi ciri anatomi daun genus Schoutenia Korth. (Malvaceae subfam. Brownlowioideae)

Nurhanim M, Noraini T, Chung R, Nurul-Aini, Ruzi A

Sains Malaysiana, 2014;43:331-338.

Kajian anatomi dan mikromorfologi daun telah dijalankan ke atas lima takson dalam genus Schoutenia Korth. (Malvaceae subfam. Brownlowioideae). Lima takson yang dipilih dalam kajian ini ialah S. kunstleri, S. leprosula, S. accrescens subsp. accrescens, S. accrescens subsp. borneensis dan S. accrescens subsp. stellata. Kajian anatomi melibatkan kaedah hirisan dengan mikrotom gelongsor pada bahagian petiol, lamina, tulang daun dan tepi daun, kaedah penjernihan lamina dan kaedah siatan epidermis daun, penjernihan dengan larutan peluntur, pewarnaan dengan Safranin dan Alcian Blue, pelekapan Canada Balsam dan cerapan bawah mikroskop cahaya. Kajian mikromorfologi melibatkan kaedah pendehidratan, titik pengeringan kritikal, saduran emas dan cerapan bawah mikroskop imbasan elektron. Objektif kajian ialah untuk melihat nilai taksonomi ciri anatomi dan mikromorfologi daun dalam genus yang dikaji. Hasil kajian menunjukkan terdapat sembilan ciri sepunya, tujuh ciri variasi yang boleh digunakan untuk pembezaan spesies dan dua ciri diagnostik yang boleh digunakan untuk pengecaman spesies. Ciri tersebut ialah corak hiasan kutikel pada S. kunstleri dan juga kehadiran sel kolenkima lamela pada S. accrescens subsp. stellata. Hasil kajian menunjukkan ciri anatomi serta mikromorfologi daun dalam genus Schoutenia mempunyai nilai taksonomi terutama dalam pembezaan dan pengecaman pada peringkat spesies dan subspesies.

Matched MeSH terms: Alcian Blue
Fulltext Effect of Collagen and GelMA on Preservation of the Costal Chondrocytes' Phenotype in a Scaffold in vivo

Isaeva EV, Kisel AA, Beketov EE, Demyashkin GA, Yakovleva ND, Lagoda TS, et al.

Sovrem Tekhnologii Med, 2023;15(2):5-16.
PMID: 37389022 DOI: 10.17691/stm2023.15.2.01

The aim of the study was to compare type I collagen-based and methacryloyl gelatin-based (GelMA) hydrogels by their ability to form hyaline cartilage in animals after subcutaneous implantation of scaffolds.
MATERIALS AND METHODS: Chondrocytes were isolated from the costal cartilage of newborn rats using 0.15% collagenase solution in DMEM. The cells was characterized by glycosaminoglycan staining with alcian blue. Chondrocyte scaffolds were obtained from 4% type I porcine atelocollagen and 10% GelMA by micromolding and then implanted subcutaneously into the withers of two groups of Wistar rats. Histological and immunohistochemical studies were performed on days 12 and 26 after implantation. Tissue samples were stained with hematoxylin and eosin, alcian blue; type I and type II collagens were identified by the corresponding antibodies.
RESULTS: The implanted scaffolds induced a moderate inflammatory response in both groups when implanted in animals. By day 26 after implantation, both collagen and GelMA had almost completely resorbed. Cartilage tissue formation was observed in both animal groups. The newly formed tissue was stained intensively with alcian blue, and the cells were positive for both types of collagen. Cartilage tissue was formed among muscle fibers.
CONCLUSION: The ability of collagen type I and GelMA hydrogels to form hyaline cartilage in animals after subcutaneous implantation of scaffolds was studied. Both collagen and GelMA contributed to formation of hyaline-like cartilage tissue type in animals, but the chondrocyte phenotype is characterized as mixed. Additional detailed studies of possible mechanisms of chondrogenesis under the influence of each of the hydrogels are needed.

Matched MeSH terms: Alcian Blue
Fulltext Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture

Bohari SP, Grover LM, Hukins DW

J Tissue Eng, 2015 Nov 19;6:2041731415615777.
PMID: 26668710 DOI: 10.1177/2041731415615777

This study evaluated the effect of pulsed low-intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by human dermal fibroblasts encapsulated in alginate. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content, dimethylmethylene blue assay for glycosaminoglycan content and scanning electron microscopy were performed on the encapsulated cells treated with pulsed low-intensity ultrasound and a control group that remained untreated. Pulsed low-intensity ultrasound showed a significant effect on cell proliferation and collagen deposition but no consistent pattern for glycosaminoglycan content. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both treated and control groups. These results suggest that pulsed low-intensity ultrasound alone shows a positive effect on cell proliferation and collagen deposition even without growth factor supplements.

Matched MeSH terms: Alcian Blue
Mucosal responses of brown-marbled grouper Epinephelus fuscoguttatus (Forsskål, 1775) following intraperitoneal infection with Vibrio harveyi

Chieng CCY, Daud HM, Yusoff FM, Thompson KD, Abdullah M

J Fish Dis, 2020 Oct;43(10):1249-1258.
PMID: 32830331 DOI: 10.1111/jfd.13222

Groupers are popular aquaculture species in South-East Asia, but their cultivation is affected by infectious disease outbreaks. Mucosa-associated lymphoid tissues provide a first-line defence against pathogens; however, few studies are available relating to cellular or proteomic responses of mucosal immunity in grouper. Skin, gill and intestine were sampled from brown-marbled grouper Epinephelus fuscoguttatus (Forsskål, 1775) at 4 and 96 hr post-infection (hpi) and 7 days post-infection (dpi) following intraperitoneal infection with Vibrio harveyi, and stained with haematoxylin/eosin and Alcian Blue/periodic acid-Schiff. Skin mucus was analysed by 2D-gel electrophoresis, and proteins modulated by the bacterial infection identified. In the infected fish, significant increases in sacciform cells in skin and increased levels of nucleoside diphosphate kinase in mucus were detected at 4 hpi. At 96 hpi, goblet cells containing acidic mucins significantly increased in the intestine, while those containing mixed mucins increased in skin and gills of infected fish. Proteasome subunit alpha type-I and extracellular Cu/Zn superoxide dismutase levels also increased in mucus. Rodlet and mast cells did not appear to respond to the infection. Mucosal tissues of grouper appeared actively involved in response to Vibrio infection. This information may help future research on improving grouper health, production and vaccine development.

Matched MeSH terms: Alcian Blue
Alterations in mucin type: an indicator for suspicion of malignant gastric transformation

Cheah PL, Ramachandran K

Malays J Pathol, 1994 Jun;16(1):39-42.
PMID: 16329574

Mucins are produced by both benign and malignant gastric epithelium. In general, mucins can be classified into neutral and acidic mucins. The latter are of 2 major types, sulphated (sulphomucins) and carboxylated (sialomucins). A retrospective study was initiated at the Department of Pathology, University Hospital, Kuala Lumpur to histochemically study the mucin profiles of cases of intestinal (IGC) and diffuse (DGC) types of gastric carcinoma in Malaysian patients to determine whether a significant change of mucin type occurs in the event of malignant transformation. 42 IGC and 37 DGC were subjected to alcian blue-periodic acid Schiff and high iron diamine-alcian blue histochemical staining. In addition, 18 cases of gastrectomies performed for benign lesions in the stomach served as normal controls. The number of cases of IGC and DGC which exhibited sulphomucin production was significantly increased (p < 0.001) compared to normal controls. Also, the number of cases of DGC which produced neutral mucin were significantly less (p < 0.05) than the control group. However, there was no significant difference between the number of IGC and DGC cases which demonstrated sialomucin production and normal controls. It appears that while not pathognomonic, a lack of neutral mucin production should alert the pathologist to the possibility of a gastric malignancy, in particular DGC. The likelihood of a malignant lesion would be further supported if there is an increased sulphomucin production.

Matched MeSH terms: Alcian Blue