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A report on 102 rubber-band ligations of internal hemorrhoids

An HK

Med J Malaysia, 1977 Dec;32(2):161-4.
PMID: 614485
Matched MeSH terms: Ligation/methods
Fulltext Ankle pseudoaneurysm: distal posterior tibial artery pseudoaneurysm in a child following blunt trauma

Aimanan K, Chea CH, Lim KY, Damodaran A

BMJ Case Rep, 2019 May 24;12(5).
PMID: 31129637 DOI: 10.1136/bcr-2018-228255

Pseudoaneurysm of the ankle involving the posterior tibial artery is a rare presentation with only four paediatric cases previously reported in English literature. We report a new case following blunt trauma with the clinical presentation and management strategy. A 6-year-old boy presented with a pulsatile swelling at the medial aspect of ankle following a history of blunt trauma 3 weeks ago. Imaging confirmed pseudoaneurysm involving the distal posterior tibial artery. Excision of the pseudoaneurysm was performed without any complication.

Matched MeSH terms: Ligation/methods*
Efficiency of ligation-mediated PCR and TAIL-PCR methods for isolation of RbcS promoter sequences from green microalgae Ankistrodesmus convolutus

Thanh T, Chi VT, Abdullah MP, Omar H, Napis S

Mol Biol (Mosk), 2012 Jan-Feb;46(1):64-70.
PMID: 22642102

Isolation of promoter sequences from known gene sequences is a tedious task in genome-related research. An efficient method of obtaining the promoter sequences is necessary in order to successfully use targeted promoters for genetic manipulations. Here, efficiency and usefulness of two PCR-based methods, namely: ligation-mediated PCR and thermal asymmetric interlaced (TAIL) PCR, for isolation of promoter sequences of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) gene from green microalgae Ankistrodesmus convolutus (A. convolutus) were evaluated. The results showed that the amplification efficiency of TAIL-PCR was higher than that of the ligation-mediated PCR method, i.e. the amplified promoter fragments of 1.2 and 0.8 kb in length or promoter sequences of 813 and 606 bp (after eliminating the unreadable sequences). The use of TAIL-PCR described here presents a low cost and efficient strategy for the isolation of promoter sequences of known genes, especially in GC-rich regions, and species with little or no available genome information such as A. convolutus.

Matched MeSH terms: Ligation/methods*
Postoperative pain following laser fistula closure versus ligation of the intersphincteric fistula tract: A prospective double-blinded randomized controlled trial

Low JQL, Rajandram R, Aziz MRA, Roslani AC

World J Surg, 2024 Aug;48(8):1990-1999.
PMID: 38844401 DOI: 10.1002/wjs.12242

BACKGROUND: Prior studies focus primarily on surgical outcomes of anal fistula treatment, such as healing rates, rather than patient-reported outcomes, such as postoperative pain, which could influence surgical choice.
OBJECTIVE: To compare pain scores at 6 and 24 h postoperatively between laser closure and ligation of the intersphincteric tract for anal fistula.
DESIGN: Prospective, double-blinded randomized controlled trial.
SETTINGS: A quaternary hospital in Malaysia.
PATIENTS: Patients aged 18-75 years with high transsphincteric fistulas.
INTERVENTION: Fistula laser closure versus ligation of the fistula tract (LIFT) treatment.
MAIN OUTCOME MEASURES: Pain scores, continence, quality of life (QOL), operative time, and treatment failure were compared using chi-square, Fisher's exact test, student t-test, or Mann-Whitney with p 0.05).
LIMITATIONS: Patients with prior fistula surgery (approximately 20%) led to heterogeneity. The total laser energy delivered varied depending on fistula anatomy.
CONCLUSION: Laser fistula closure is an alternative to LIFT, with similar postoperative pain and shorter operative time despite more complex fistula anatomy in the laser arm, with a greater improvement in QOL.
TRIAL REGISTRATION: ClinicalTrials.gov: NCT06212739.

Matched MeSH terms: Ligation/methods