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To date, more than 50 Eimeria spp. have been isolated from marsupials of the family Macropodidae. Although 18 species of Eimeria have been previously detected from multiple animal species belonging to the genus Macropus of the family, limited genetic analyses of the parasites are available, and their pathogenicity remains unclear. Here, we report the isolation of Eimeria spp. from a zoo specimen of red-necked wallaby (Macropodidae; Macropus rufogriseus). Specifically, two distinct types of Eimeria oocysts were recovered, one from the feces before treatment with an anthelmintic and the second from the intestinal contents after death of the animal. The oocysts obtained from the two sources were morphologically identified as E. hestermani and E. prionotemni, respectively. We successfully determined partial gene sequences from the two isolates, including segments of the 18S rRNA genes, and for the first time have used phylogenetic analyses of these sequences to assign the species to distinct clades. In combination with further genetic data, these results are expected to help elucidate the pathogenicity and host ranges of Eimeria spp. within the respective family and genus.

Matched MeSH terms: Eimeria/isolation & purification*

Detection of Eimeria oocysts in chicken feces using flotation recovery with sucrose or saturated saline solution

Takano A, Morinaga D, Teramoto I, Hatabu T, Kido Y, Kaneko A, et al.

Acta Parasitol, 2025 Jan 09;70(1):17.

PMID: 39789311 DOI: 10.1007/s11686-024-00960-6

PURPOSE: Flotation methods are widely used to detect oocysts/cysts of protozoans and eggs of helminths, except trematodes. However, details regarding the concentration and recovery rates of these parasites are poorly understood.

METHODS: Using Eimeria tenella oocysts as a model parasite, the present study evaluated three check points: (1) the proportion of parasites that remain floating in flotation solution (sucrose or saturated saline) during centrifugation, (2) the proportion of oocysts that naturally float after addition of flotation solution after centrifugation, and (3) the rate of recovery on cover slips after completion of the flotation protocol.

RESULTS: After centrifugation in sucrose solution and saturated saline solution, 82.4% and 60.3% of oocysts floated, respectively. After addition of flotation solution after the final centrifugation step, the recovery rates for oocysts that naturally floated again for 30 min in sucrose and saturated saline were 39.2% and 38.2%, respectively. The recovery rate on cover slips as the final step after performing a commonly used flotation method was 36.4% in sucrose solution (the rate for saturated saline solution could not be assessed due to rapid crystallization).

CONCLUSION: Our results suggest that floating oocysts could have become dispersed by the addition of flotation solution, and not all of these oocysts remained floating after an additional 30 min of settling time although collection on cover slips could be effective for accurate recovery.

Matched MeSH terms: Eimeria/isolation & purification

New species of Eimeria (Protozoa: Eimeriidae) from Malaysian squirrels

Colley FC, Mullin SW

J. Protozool., 1971 Aug;18(3):400-2.

PMID: 5167227

Matched MeSH terms: Eimeria/isolation & purification

Isospora hylomysis and Eimeria bentongi n. spp. (Protozoa: Eimeriidae) from the lesser gymnure Hylomys suillus Müller, 1839 in Malaysia

Colley FC, Mullin SW

Southeast Asian J Trop Med Public Health, 1971 Sep;2(3):289-91.

PMID: 5168250

Matched MeSH terms: Eimeria/isolation & purification

Coccidian parasites (Protozoa: Eimeriidae) of the lesser mouse-deer, Tragulus javanicus. I. Descriptions of three new Eimeria species

Mullin SW, Colley FC

Southeast Asian J Trop Med Public Health, 1971 Dec;2(4):441-3.

PMID: 5169144

Matched MeSH terms: Eimeria/isolation & purification

Coccidia of Malaysian mammals: new host records and descriptions of three new species of Eimeria

Mullin SW, Colley FC, Stevens GS

J. Protozool., 1972 May;19(2):260-3.

PMID: 5032224

Matched MeSH terms: Eimeria/isolation & purification

Evaluation of the detection method by a flotation method using a wire loop for gastrointestinal parasites

Takano A, Morinaga D, Teramoto I, Hatabu T, Kido Y, Kaneko A, et al.

Vet Med Sci, 2024 Sep;10(5):e70007.

PMID: 39207196 DOI: 10.1002/vms3.70007

Infections by gastrointestinal parasites are found in a variety of animals worldwide. For the diagnosis of such infections, the flotation method is commonly used to detect parasitic microorganisms, such as oocysts or eggs, in feces. Instead of adding a flotation solution after the final centrifugation step and using a cover slip to collect the parasites, the method using a wire loop for the recovery of the organisms has been reported as one of alternative methods. However, the recovery rates of microorganisms from the flotation method have not been analysed. In the present study, the utility of a flotation method with the use of a wire loop of 8 mm in diameter (the loop method) was evaluated using different numbers of E. tenella oocysts and Heterakis gallinarum eggs, and chicken fecal samples collected at the farms. Consequently, we found that the oocysts and eggs in tubes could be collected at a ratio of 2.00 to 3.08. Thus, our results indicate that the loop method is a simple and time saving method, implicating the application for the estimated OPG/ EPG (Oocysts/Eggs per gram) of the samples.

Matched MeSH terms: Eimeria/isolation & purification

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