Browse publications by year: 2022

  1. Aamir K, Sugumar V, Khan HU, Looi CY, Juneja R, Waqas M, et al.
    Toxicol Res, 2022 Apr;38(2):159-174.
    PMID: 35419271 DOI: 10.1007/s43188-021-00092-3
    Chebulinic acid (CA) is an ellagitannins isolated from the dried fruits of Terminalia chebula with diverse pharmacological activities. The present study focused on the acute toxicity of CA in normal Sprague Dawley (SD) rats. CA was administered via oral gavage to different groups in 300 and 2000 mg/kg body weight and vehicle respectively. All the animals were monitored carefully for any physiological or behavioral changes for 14 days. On day 15th animals were euthanized and blood was collected for hematological and biochemical analysis. Different tissues were collected for histopathological study using four different staining techniques (hematoxylin and eosin, Masson's trichrome, periodic acid Schiff and picro sirius red) to observe any pathological alterations. The results highlighted no morbidity and mortality after oral ingestion of CA (300 and 2000 mg/kg). Food and water consumption, body weight, relative organ weight, hematological and biochemical parameters were normal without any gross pathological lesions in harvested tissues. The outcome of the current study supported safety of CA even at high dose. However, further detailed study is required on experimentally disease model to unfold its therapeutic potential in laboratory animals.
  2. Oginawati K, Susetyo SH, Rahmawati SI, Kurniawan SB, Abdullah SRS
    Toxicol Res, 2022 Apr;38(2):149-157.
    PMID: 35419272 DOI: 10.1007/s43188-021-00094-1
    This study aims to determine the distribution of organochlorine pesticide pollution in water, sediments, mollusks, and fish at Saguling Dam as baseline data of organochlorine pollution. Samples were obtained from 12 locations, with 9 and 3 sampling points inside and outside the dam, respectively. Measurement of organochlorine residues was carried out using methods of extraction, purification, evaporation, and gas chromatography. Results showed the presence of several types of organochlorine compounds, namely, lindane, aldrin, dieldrin, heptachlor, dichlorodiphenyltrichloroethane (DDT), and endosulfan. Aldrin was dominant in water (2-37 μg/L) and sediments (2-1438 μg/L), while DDT and heptachlor were dominant organochlorine compounds in mollusks (13-2758 µg/L) and fish (11-104 μg/L), respectively. Sediments demonstrated higher organochlorine concentrations than water, mollusk, and fish. The distribution of organochlorine was affected by land use around the Citarum watershed and pollutant input from tributaries.
  3. Goswami K, Mazumdar I
    Indian J Clin Biochem, 2022 Apr;37(2):206-211.
    PMID: 35463105 DOI: 10.1007/s12291-021-00975-0
    'Holi' is an Indian festival with a great cultural context, that is celebrated across the world at the onset of spring by applying dry powder of vibrant colours on friends and family. In ancient times holi colours were prepared from different spring flowers, but in modern times, these natural colours have been replaced by commercial industrial dyes prepared by chemical processes. Even products that claim to use organic colours, use synthetic pigments to enhance the brightness of hues. Such synthetic holi colours are sold as herbal colours, in an unregulated manner, in local markets, and no checks can be enforced on the product composition. Also, the quality and the amount of information about the ingredients of the particular packets are missing. These colours sold in the local market often contain hazardous chemicals such as endotoxins, and heavy metals, like lead, potentially causing moderate to severe health problem. Holi colour samples were randomly collected from different sites in Kolkata, India. Red, pink, violet, green and yellow coloured powders were obtained. The powders were prepared and analysed for lead content by Inductively Coupled Plasma-Mass Spectrometric method. Analysis of endotoxin content of different holi colours was also performed by Limulus Amebocyte Lysate test. The lead content was found to be almost 2 times higher in the holi colours, with yellow pigment having the highest concentration, than FDA Standard for maximum permissible limit in cosmetics, which was taken as a reference for safety limit of lead that is dermatologically applicable. The endotoxin levels are alarmingly high, with almost 35 times the FDA reference for dermatological safety limit. Special attention should be given to lead and endotoxin levels in holi colours as their consequences pose serious health threats. Therefore, quality control measures should be recommended for them, in par with products designed for long-term contact with the skin.
  4. Moorthy PSK, Sakijan AS
    Indian J Thorac Cardiovasc Surg, 2022 Apr;38(Suppl 1):122-131.
    PMID: 35463696 DOI: 10.1007/s12055-021-01292-0
    Malperfusion syndrome (MPS) complicating acute type A aortic dissection (ATAAD) poses a continuing challenge and management dilemma for cardiovascular surgeons. MPS may involve any of the major arterial side branches resulting in myocardial, cerebral, spinal cord, visceral, and/or limb ischemia with varying frequency and severity. Despite the continuous improvement in diagnosis and management strategies for MPS with ATAAD, clinical outcomes remain poor and the optimal therapy is still debatable. The present review aimed to assess current evidence on ATAAD patients with MPS and how best to handle the challenge.
  5. Norman HH, Hadie SNH, Yaacob NM, Kasim F
    Med Sci Educ, 2022 Apr;32(2):399-409.
    PMID: 35528310 DOI: 10.1007/s40670-022-01517-w
    This study aimed to measure medical students' perceptions of the anatomy education environment and determine its association with their learning approaches. First- and second-year undergraduate medical students (N = 234) completed the Anatomy Education Environment Measurement Inventory and the Approaches and Study Skills Inventory for Students. Results revealed that the students' perceptions of all the factors in the anatomy education environment were positive except for two areas-the anatomy learning resources and quality of histology learning facilities. The majority of students in the first year (62.3%) and second year (43.7%) adopted a deep learning approach. There was no significant association between most of the factors in the anatomy education environment and the students' learning approaches, except for the students' intrinsic interest in learning anatomy factor (p = 0.032). The anatomy education environment was positive for the medical students, and there was a prevalence of a deep learning approach among them. However, most of the factors in the anatomy education environment did not influence the students' learning approach. Given that the students' learning approach is a multifactorial construct, investigating the relationship between these two variables in longitudinal time points would provide better insight into the association between the anatomy educational climate and students' learning approaches.
  6. Rajah R, Tang YW, Ho SC, Tan JL, Mat Said MS, Ooi LH
    Hosp Pharm, 2022 Apr;57(2):217-222.
    PMID: 35601709 DOI: 10.1177/00185787211010164
    Background: Inhaler technique errors can affect the disease outcome through reduced deposition of medication in the lungs with certain errors were more likely to deter the optimum drug from reaching the lung. The objective of this study was to evaluate the pressurized metered-dose inhaler (pMDI) critical technique errors among asthma and COPD patients, and to investigate the predictors of those errors. Methods: A cross-sectional, observational, and multi-centered study conducted in 3 major hospitals in the state of Penang, Malaysia. Asthma and COPD patients aged 18 years or older consented to the study were consecutively recruited if they were at least on 1 pMDI without mouthpiece spacer or facemask space. Direct observation of the patient's technique was recorded using a standard checklist and certain steps in the checklist were pre-determined as critical steps. The number of patients missed or incorrectly performed the steps including the critical steps were documented. Using logistic regression, the factors associated with the critical errors were determined, and the correlation was evaluated between age and pMDI knowledge score with critical inhalation steps score. Results: Of 209 patients observed, only less than half (40.7%) could perform all the critical pMDI steps correctly. Low education level was the only factor associated with critical inhaler errors. The increased knowledge of their inhaled medication was found to positively correlate with the correct critical inhalation steps. The critical errors also increase with the increased age of patients. Conclusion: The majority of the study population make inhalation error particularly in critical steps. Training and education intervention programs may help patients with lower education level, as well as those of advanced age and lack of knowledge of their inhaled medication because those populations are at a greater risk of committing critical errors in their use of pMDI.
  7. Madin J, Talaha NHM, Lal MTM, Ransangan J, Yoshida T, Gallagher JB
    Zool Stud, 2022;61:e79.
    PMID: 37007816 DOI: 10.6620/ZS.2022.61-79
    Fin whales are a cosmopolitan species found in the largest water masses of the world. In Malaysia, as well as other tropical countries in the Southeast Asian region, literature on fin whales is limited, and as a result, there is confusion regarding their distribution range in the region. This study utilizes the fresh tissue of the skin and blubber of a dead fin whale that was stranded in Sabah (Borneo, Malaysia) on the coast of the South China Sea to confirm the species identity, possible properties of the species' diet, and any trace element contamination. The DNA profile results confirmed that the whale belonged to Balaenoptera physalus. Further investigation of its cytochrome b gene sequence indicated that it was closely related to the southern fin whale (Balaenoptera physalus quoyi). This finding indicates that fin whales indeed migrate to warm tropical waters and that their continuous global distribution spans the equatorial region. The dominant fatty acids, such as C18:0, C16:1, C18:1N9T and C16:0 profiles, were consistent with the pelagic plankton diet that the whale would have had during its migration in the tropical waters of the South China Sea. The whales are likely pelagic feeders and thus need to be offshore, which would explain why they are rarely seen in shallow coastal areas during migration in these waters. The concentrations of K, Ca, Sc, Mg and Al ranged from 0.45 μg g-1 to 7.80 μg g-1, while Cr, Cd, As and Pb were either very low or could not be detected. This is consistent with concentrations of trace elements previously reported for other baleen whale genera from the Southern Ocean. Our study demonstrates the importance of the South China Sea as a migration route for the southern fin whale, since it is a rich food source with relatively low contaminant levels. The South China Sea is therefore well-suited to ensure these whales' survival during migration.
  8. Hata H, Lavoué S, Motomura H
    Zool Stud, 2022;61:e58.
    PMID: 37007820 DOI: 10.6620/ZS.2022.61-58
    The Bengal Spined Anchovy, Stolephorus taurus sp. nov. is described from 21 specimens from the northern Bay of Bengal. The new species closely resembles Stolephorus dubiosus Wongratana, 1983, which is redescribed. Both species have a predorsal scute, spine on the pelvic scute, long maxilla posteriorly slightly short of or just reaching the posterior margin of the opercle, 25 or more gill rakers on the lower limb of the first gill arch, and double black lines on the dorsum posterior to the dorsal fin. However, the new species differs from S. dubiosus in having a longer pelvic fin with the posterior tip of the depressed fin beyond vertical through the dorsal-fin origin (vs. usually not reaching to vertical through dorsal-fin origin), longer pectoral fin, second dorsal-and third dorsal-fin rays, second anal-and third anal-fin rays, and greater interorbital width. Stolephorus taurus sp. nov. is closely related to Stolephorus baganensis Delsman, 1931 and S. dubiosus, although at least 2% mean p-distance divergence in the mitochondrial cytochrome c oxidase subunit I (COI) gene separates each of the three species. A phylogenetic reconstruction of the evolution of the number of prepelvic scutes within Stolephorus indicated that having six scutes was the most likely ancestral condition in the genus, and was later reduced in the evolution of Stolephorus to five or four scutes. One such reduction occurred recently in the lineage of Stolephorus taurus sp. nov.
  9. Abdullah-Fauzi NAF, Karuppannan KV, Mohd-Radzi NHS, Gani M, Mohd-Ridwan AR, Othman N, et al.
    Zool Stud, 2022;61:e60.
    PMID: 37007822 DOI: 10.6620/ZS.2022.61-60
    The world's largest terrestrial mammal, Asian elephants, are known to have enormous feeding needs. Several factors such as season, sex, age, and daily activities influence the amount of food required by an individual. Generally, captive elephants have a limited choice of food on a daily basis compared with that of elephants in the wild. Elephants in captivity are fed according to a prepared feeding schedule, whereas wild elephants are free to choose the type of plants that they consume in their natural habitat. In the past, ecological observations have been widely used to determine the diet of wild elephants. However, the molecular approach has never been carried out. In the present study, we aimed to; 1) identify the plant diet of wild Asian elephants in Taman Negara National Park (TNNP) according to their sex and age using high-throughput DNA metabarcoding; and 2) determine the dietary formulation of captive elephants based on the generated plant metabarcoding database. DNA was extracted from 24 individual fecal samples collected using noninvasive sampling techniques from TNNP and the National Elephant Conservation Centre (NECC) Kuala Gandah. Seven pooled samples from male adult, female adult, male subadult, female subadult, male juvenile, female juvenile, and captive elephants were amplified and sequenced targeting the trnL region (50-150 base pairs). The CLC Genomic Workbench and PAST 4.02 software were used for data analysis. In total, 24 orders, 41 families, 233 genera, and 306 species of plants were successfully detected in the diet of the Asian elephants. The most abundant plant genera consumed were Sporobolus (21.88%), Musa (21.48%), and Ficus (10.80%). Plant variation was lower in samples from male elephants than in those from female elephants. The plant species identified were correlated with the nutrient benefits required by elephants. Adults and subadults consumed more plant species than were consumed by juvenile elephants. However, there was no significant difference between ages and sexes. The findings of this study can be used as guidance by the Department of Wildlife and National Parks for the management of captive elephants, especially in NECC Kuala Gandah.
  10. Song CV, Yip CH, Mohd Taib NA, See MH, Teoh LY, Monninkhof EM, et al.
    JCO Glob Oncol, 2022 Mar;8:e2100314.
    PMID: 35245099 DOI: 10.1200/GO.21.00314
    PURPOSE: Addressing unwarranted clinical variation in oncology practices is expected to lead to improved cancer outcomes. Particularly, the application and impact of treatment guidelines on breast cancer outcomes are poorly studied in resource-limited settings. We measured adherence to a set of locally developed adjuvant treatment guidelines in a middle-income setting. Importantly, the impact of guidelines adherence on survival following breast cancer was determined.

    METHODS: Data of 3,100 Malaysian women with nonmetastatic breast cancer diagnosed between 2010 and 2017 were analyzed. Adherence to the Malaysian Clinical Practice Guidelines for Management of Breast Cancer second Edition was measured. Outcomes comprised overall survival and event-free survival.

    RESULTS: Guideline adherence for chemotherapy, radiotherapy, hormonal therapy, and targeted therapy were 61.7%, 79.2%, 85.1%, and 26.2%, respectively. Older age was generally associated with lower adherence to guidelines. Compared with patients who were treated according to treatment guidelines, overall survival and event-free survival were substantially lower in patients who were not treated accordingly; hazard ratios for all-cause mortality were 1.69 (95% CI, 1.29 to 2.22), 2.59 (95% CI, 1.76 to 3.81), 3.08 (95% CI, 1.94 to 4.88), and 4.48 (95% CI, 1.98 to 10.13) for chemotherapy, radiotherapy, hormone therapy, and targeted therapy, respectively. Study inferences remain unchanged following sensitivity analyses.

    CONCLUSION: Our study findings appear to suggest that adherence to treatment guidelines that have been adapted for resource-limited settings may still provide effective guidance in improving breast cancer outcomes.

    MeSH terms: Combined Modality Therapy; Female; Humans; Proportional Hazards Models; Guideline Adherence
  11. Mohd Mujar NM, Dahlui M, Emran NA, Hadi IA, Yan YW, Arulanantham S, et al.
    JCO Glob Oncol, 2022 Mar;8:e2100250.
    PMID: 35286134 DOI: 10.1200/GO.21.00250
    PURPOSE: The aim of this study is to determine the pathway that women follow for Breast Cancer Care (BCC) and the time intervals from symptom discovery to treatment initiation and to develop a quality matrix framework.

    METHODS: A retrospective cohort study was conducted at six tertiary centers in Malaysia. All women with newly diagnosed breast cancer were interviewed, and a medical records review was conducted using a structured questionnaire. The BCC timeliness framework showed that the total time between a woman discovering their first breast changes and the date of initial treatment was divided into three distinct intervals: presentation interval, diagnostic interval, and treatment interval. Four diagnosis subintervals, referral, biopsy, report, and diagnosis resolution intervals, were also looked into.

    RESULTS: The BCC timeliness framework was used to capture important time points. The median total time, presentation interval, diagnostic interval, and treatment interval were 4.9 months (range, 1 month to 10 years), 2.4 months (range, 7 days to 10 years), 26 days (range, 4 days to 9.3 months), and 21 days (range, 1 day to 7.2 months), respectively. Meanwhile, the median time for the diagnosis subinterval of referral, biopsy, report, and diagnosis resolution was 8 days (range, 0 day to 8 months), 0 day (range, 0 day to 20 days), 7 days (range, 3 days to 3.5 months), and 4 days (range, 1 day to 1.8 months), respectively.

    CONCLUSION: The BCC timeliness framework is based on the current sequenced trajectory of the BCC journey. Clarity in the measurement of timeliness provides a standardized language for monitoring and outcome research. It can serve as a quality indicator for community and hospital-based breast cancer programs.

    MeSH terms: Breast; Female; Humans; Surveys and Questionnaires; Referral and Consultation; Retrospective Studies
  12. Gong L, Sheng J
    Front Psychol, 2022;13:906297.
    PMID: 35936271 DOI: 10.3389/fpsyg.2022.906297
    The purpose of this study was to discuss the differences in the parameters of exercise health beliefs among college students of different genders, and assess the relationship between demographic factors and parameters of exercise health beliefs, and examine the relationship between exercise self-efficacy and internal components of exercise health beliefs. A total of 313 Chinese college students from the Sichuan University of Arts and Science completed the exercise health belief scale voluntarily under the tutor's explanation. The results showed that compared with female students, male students have higher perceived benefits and self-efficacy of exercise and lower perceived subjective and objective barriers. Monthly family income has a significant positive correlation with exercise self-efficacy and a negative correlation with perceived subjective barriers to exercise disorder. Exercise self-efficacy has a positively correlated with perceived benefits and perceived severity and a significant negative correlation with perceived subjective and objective barriers. From the structural equation model, we found that family income no longer significantly impacted exercise self-efficacy. Within the exercise health belief items, we found only that there were negative relationship between perceived subjective barriers and exercise self-efficacy. According to the study, our findings provide a new psychological angle for understanding the exercise condition of college students and the restraining factors and provide new insights into increasing exercise self-efficacy to lower the subjective barriers to exercise. Future studies will focus on evaluating the relationship between exercise health belief components and college Students' physical activity levels and exercise behaviors.
  13. Lee SH, Moorthy R, Nagala S
    Br J Surg, 2022 May 16;109(6):497-502.
    PMID: 35576381 DOI: 10.1093/bjs/znac072
    BACKGROUND: The IDEAL Framework is a scheme for safe implementation and assessment of surgical innovation. The transoral endoscopic thyroidectomy vestibular approach (TOETVA) is a new innovation in thyroid surgery that eliminates the need for a cervical incision. Despite considerable interest and adoption worldwide, significant scepticism remains regarding the outcomes and cost-effectiveness for healthcare systems. The aim of this narrative review was to appraise the available literature and examine whether TOETVA has progressed in line with the IDEAL Framework.

    METHODS: A literature review of PubMed with a focus on historical and landmark studies was undertaken to classify the evidence according to the different stages of the IDEAL Framework.

    RESULTS: Several different transoral approaches were developed by a small of number of surgeon-innovators on animals and cadavers, and subsequently in first-in-human studies. The trivestibular approach emerged as the safest technique, with further refinements of this technique culminating in TOETVA. The basic steps and indications for this technique have been standardized and it is now being replicated by early adopters in many centres worldwide. The development of TOETVA has closely aligned with the IDEAL Framework, and is currently at stage 2B (Exploration).

    CONCLUSION: There is need for multi-institutional collaborations and international registry studies to plan high-quality randomized trials comparing TOETVA with other remote-access approaches and collect long-term follow-up data. In countries where TOETVA has yet to be adopted, the IDEAL Framework will be a useful roadmap for government regulators and professional societies to evaluate, regulate, and provide best practice recommendations for the adoption of this technique.

    MeSH terms: Endoscopy; Humans; Parathyroid Glands; Thyroid Gland/surgery; Operative Time
  14. Pekar JE, Magee A, Parker E, Moshiri N, Izhikevich K, Havens JL, et al.
    Science, 2022 Aug 26;377(6609):960-966.
    PMID: 35881005 DOI: 10.1126/science.abp8337
    Understanding the circumstances that lead to pandemics is important for their prevention. We analyzed the genomic diversity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) early in the coronavirus disease 2019 (COVID-19) pandemic. We show that SARS-CoV-2 genomic diversity before February 2020 likely comprised only two distinct viral lineages, denoted "A" and "B." Phylodynamic rooting methods, coupled with epidemic simulations, reveal that these lineages were the result of at least two separate cross-species transmission events into humans. The first zoonotic transmission likely involved lineage B viruses around 18 November 2019 (23 October to 8 December), and the separate introduction of lineage A likely occurred within weeks of this event. These findings indicate that it is unlikely that SARS-CoV-2 circulated widely in humans before November 2019 and define the narrow window between when SARS-CoV-2 first jumped into humans and when the first cases of COVID-19 were reported. As with other coronaviruses, SARS-CoV-2 emergence likely resulted from multiple zoonotic events.
    MeSH terms: Animals; Computer Simulation; Humans; Phylogeny; Genetic Variation; Molecular Epidemiology; Genomics/methods; Pandemics*
  15. Kalra S, Dhar M, Afsana F, Aggarwal P, Aye TT, Bantwal G, et al.
    Rev Diabet Stud, 2022 Jun 30;18(2):100-134.
    PMID: 35831938 DOI: 10.1900/RDS.2022.18.100
    The elderly population with diabetes is diverse with the majority experiencing a decline in physical and mental capabilities, impacting the entire diabetes management process. Therefore, a need for geriatric-specific guidelines, especially for the Asian population, was identified and subsequently developed by an expert panel across government and private institutions from several Asian countries. The panel considered clinical evidence (landmark trials, position papers, expert opinions), recommendations from several important societies along with their decades of clinical experience and expertise, while meticulously devising thorough geriatric-specific tailored management strategies. The creation of the ABCDE best practices document underscores and explores the gaps and challenges and determines optimal methods for diabetes management of the elderly population in the Asian region.
    MeSH terms: Aged; Asia/epidemiology; Humans
  16. Marzo RR, Bhattacharya S
    J Family Med Prim Care, 2022 Nov;11(11):6772-6777.
    PMID: 36993001 DOI: 10.4103/jfmpc.jfmpc_271_19
    INTRODUCTION: Globally, mental health today poses a significant hazard to all of us. It is more vital for students, whose survival in a highly competitive atmosphere places them under enormous pressure.

    AIM AND OBJECTIVE: This qualitative study sought to determine how mental health counsellors now address the mental health concerns of their pupils. To achieve this objective, two research questions were developed to direct this study: (1) What are the experiences of counsellors who provide support to students with mental health issues? (2) How might guidance and counselling services and programmes enhance the academic success of students with mental health issues?

    METHODOLOGY: Participants were selected from a university in a northern Malaysian neighbourhood. Several semi-structured, in-depth interviews were conducted with two counsellors to collect data.

    RESULTS: Overall, the counsellors regarded multitasking to be a hindrance to their job performance. Participants felt that their caseloads prevented them from being proactive with each student, resulting in irritation. According to the participants, the job's requirements have changed, but the number of tasks and caseload have not. This has led to feelings of exhaustion and frustration. The study revealed two important research's findings: first, there has been an increase in the number of mental health concerns, particularly anxiety and depression, among students; second, counsellors would be able to effectively support children intellectually and personally if they had appropriate staffing and professional development.

    CONCLUSION: It was concluded that the counselors regarded multitasking was a hindrance to their job performance. Participants reported an increase in the frequency of anxiety and depression exhibited by their students and they felt that additional programming with friends, family, and professors could increase students' social well-being.

  17. Mahlangu J, Abdul Karim F, Stasyshyn O, Korczowski B, Salazar B, Lucas S, et al.
    Res Pract Thromb Haemost, 2022 Feb;6(2):e12665.
    PMID: 35224416 DOI: 10.1002/rth2.12665
    BACKGROUND: rVIII-SingleChain is a recombinant single-chain factor VIII used to treat people with hemophilia A.

    OBJECTIVES: The aim of this extension study was to investigate the long-term safety and efficacy of rVIII-SingleChain prophylaxis in ≥200 previously treated patients (PTPs) with hemophilia A with ≥100 exposure days (EDs).

    METHODS: In total, 222 patients were enrolled, of which 204 rolled over from prior rVIII-SingleChain studies. The median age was 21 years (range, 2-65 years), including 155 patients ≥12 years and 67 patients <12 years. Patients continued with their previously assigned dose and regimen, or switched at the investigator's discretion. Patients were treated for a mean duration of 31 months (range, 1-47 months), the mean ED was 342 (standard deviation, 135.5), and 212 (95.5%) patients achieved >100 EDs. When the study ended, most patients were on either a prophylaxis regimen of 34.9 (17-62) IU/kg, 3×/week (N = 88; 39.6%), or 37.2 (13-65) IU/kg, 2×/week regimen (N = 72; 32.4%).

    RESULTS: Hemostatic efficacy was rated excellent or good in 87.1% of assessed bleeds. The median (range) annualized bleeding rate was 1.21 (0.0-42.6), and the annualized spontaneous bleeding rate (AsBR) was 0.32 (0.0-33.0) for prophylaxis regimens. Median AsBR was similar for patients treated 3×/week and 2×/week (0.31 and 0.30, respectively). Surgical hemostatic efficacy was rated excellent or good in 100% of surgeries. No inhibitors, anaphylactic reactions, or thromboembolic events were reported in PTPs.

    CONCLUSION: These results confirm the safety and efficacy of rVIII-SingleChain as a long-term prophylaxis treatment modality for PTPs with severe hemophilia A.

  18. Boahen A, Than LTL, Loke YL, Chew SY
    Front Microbiol, 2022;13:787119.
    PMID: 35694318 DOI: 10.3389/fmicb.2022.787119
    "Unity in strength" is a notion that can be exploited to characterize biofilms as they bestow microbes with protection to live freely, escalate their virulence, confer high resistance to therapeutic agents, and provide active grounds for the production of biofilms after dispersal. Naturally, fungal biofilms are inherently resistant to many conventional antifungals, possibly owing to virulence factors as their ammunitions that persistently express amid planktonic transition to matured biofilm state. These ammunitions include the ability to form polymicrobial biofilms, emergence of persister cells post-antifungal treatment and acquisition of resistance genes. One of the major disorders affecting vaginal health is vulvovaginal candidiasis (VVC) and its reoccurrence is termed recurrent VVC (RVVC). It is caused by the Candida species which include Candida albicans and Candida glabrata. The aforementioned Candida species, notably C. albicans is a biofilm producing pathogen and habitually forms part of the vaginal microbiota of healthy women. Latest research has implicated the role of fungal biofilms in VVC, particularly in the setting of treatment failure and RVVC. Consequently, a plethora of studies have advocated the utilization of probiotics in addressing these infections. Specifically, the excreted or released compounds of probiotics which are also known as postbiotics are being actively researched with vast potential to be used as therapeutic options for the treatment and prevention of VVC and RVVC. These potential sources of postbiotics are harnessed due to their proven antifungal and antibiofilm. Hence, this review discusses the role of Candida biofilm formation in VVC and RVVC. In addition, we discuss the application of pro-, pre-, post-, and synbiotics either individually or in combined regimen to counteract the abovementioned problems. A clear understanding of the role of biofilms in VVC and RVVC will provide proper footing for further research in devising novel remedies for prevention and treatment of vaginal fungal infections.
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