In vitro biodegradation, cytotoxicity, and biocompatibility of polylactic acid/napier cellulose nanofiber scaffold composites

Revati R 1 , Majid MSA 2 , Ridzuan MJM 3 , Mamat N 4 , Cheng EM 4 , Alshahrani HA 5

Affiliations 

  • 1 Faculty of Mechanical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Pauh Putra Campus, Arau 02600, Perlis, Malaysia; Faculty of Electronic Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Pauh Putra Campus, Arau 02600, Perlis, Malaysia
  • 2 Faculty of Mechanical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Pauh Putra Campus, Arau 02600, Perlis, Malaysia. Electronic address: shukry@unimap.edu.my
  • 3 Faculty of Mechanical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Pauh Putra Campus, Arau 02600, Perlis, Malaysia
  • 4 Faculty of Electronic Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Pauh Putra Campus, Arau 02600, Perlis, Malaysia
  • 5 Department of Mechanical Engineering, College of Engineering, Najran University, Najran 11001, Saudi Arabia
Int J Biol Macromol, 2022 Dec 31;223(Pt A):479-489.
PMID: 36368357 DOI: 10.1016/j.ijbiomac.2022.11.041

Abstract

This study aimed to evaluate the bioactivities and biocompatibilities of porous polylactic acid (PLA) reinforced with cellulose nanofiber (CNF) scaffolds. The in vitro degradation behaviors of the porous PLA/CNF scaffolds were systematically measured for up to 8 weeks in a phosphate-buffered saline medium at 37 °C. The reinforcement of CNF resisted the biodegradation of the scaffolds. The in vitro cytotoxicity and biocompatibility of the scaffolds were determined using the Beas2B American Type Culture Collection cells. The 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide cytotoxicity and proliferation tests showed that the scaffolds were non-toxic, and epithelial cells grew well on the scaffold after 7 days of culture, whereas the percentage of cell proliferation on the PLA/CNF15 scaffold was the largest, 130 %. A scratch wound-healing assay was performed to evaluate the suitability of the scaffolds for cell migration. The results demonstrated that the scaffolds exhibited good cell migration towards nearly complete wound closure.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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