Affiliations 

  • 1 Ebonyi State University Mushroom Center, Abakaliki, Nigeria. chukwu.samuel@ebsu.edu.ng
  • 2 Department of Crop Production and Landscape Management, Faculty of Agriculture and Natural Resources Management, Ebonyi State University, PMB 053, Abakaliki, Nigeria
  • 3 Department of Clinical Medicine, Ebonyi State University, Abakaliki, Nigeria
  • 4 Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM Serdang, 43400, Selangor, Malaysia
  • 5 Ebonyi State University Mushroom Center, Abakaliki, Nigeria
Sci Rep, 2022 Nov 09;12(1):19054.
PMID: 36351926 DOI: 10.1038/s41598-022-16833-9

Abstract

Mushrooms are fleshy fungi valued globally for their nutritional and medical benefits. The study was conducted at Ebonyi State University Mushroom Center, Abakaliki, to determine an optimum level of limestone (CaCO3) on the genotypes for maximum growth and yield. The experiment was carried out as a split-plot experiment in a completely randomized design (CRD) with the use of Oyster mushroom variety. The two genotypes (GI and GII) were placed in the whole plot while limestone was placed in the sub-plot which consisted of five rates of CaCO3(Og,5 g, 10 g, 15 g and 20 g). Sawdust and rice husk substrates were used at the ratio of 60:40 and sterilized for six hours at 121 °C using the steam sterilization cylinder. The media bags were off-loaded after one day and allowed to further cool for another day before inoculation. The cultured spawn was used to inoculate the media upon cooling at room temperature. Data were collected on agro-morphological parameters such as primordial initiation, stalk height, stalk diameter, number of branches, number of fruits, number of productive bags, fresh and dry weights, and subjected to analysis of variance (ANOVA). The result obtained indicated that there was a significant difference (P  0.05) in all parameters evaluated except the stalk diameter. Genotype I initiated more primordial compared to primordial initiation in genotype II and they differed significantly (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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