Displaying publications 1 - 20 of 1060 in total

  1. Chew MH, Rahman MM, Hussin S
    Pak J Med Sci, 2015;31(3):615-20.
    PMID: 26150855 DOI: 10.12669/pjms.313.6340
    Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus.
    Matched MeSH terms: Genotype
  2. Dow RA, Zhang HM
    Zootaxa, 2018 Jan 25;4375(4):567-577.
    PMID: 29690088 DOI: 10.11646/zootaxa.4375.4.6
    Yunnanosticta gen. nov. in the platystictid subfamily Sinostictinae is described from Yunnan, China. The genotype is Yunnanosticta wilsoni sp. nov., described here (holotype ♂ from Tongbiguan, Yingjiang County, Dehong Dai Jingpo Autonomous Prefecture, Yunnan, China, 23 vi 2015, leg. H.M. Zhang, to be deposited in the Natural History Museum, London). Yunnanosticta cyaneocollaris sp. nov. (holotype ♂ from Tongbiguan, Yingjiang County, Dehong Dai Jingpo Autonomous Prefecture, Yunnan, China, 23 vi 2015, leg. H.M. Zhang, to be deposited in the Natural History Museum, London) is also described.
    Matched MeSH terms: Genotype
  3. Oladosu Y, Rafii MY, Magaji U, Abdullah N, Miah G, Chukwu SC, et al.
    Biomed Res Int, 2018;2018:8936767.
    PMID: 30105259 DOI: 10.1155/2018/8936767
    The associations among yield-related traits and the pattern of influence on rice grain yield were investigated. This evaluation is important to determine the direct and indirect effects of various traits on yield to determine selection criteria for higher grain yield. Fifteen rice genotypes were evaluated under tropical condition at five locations in two planting seasons. The experiment was laid out in a randomized complete block design with three replications across the locations. Data were collected on vegetative and yield components traits. The pooled data based on the analysis of variance revealed that there were significant differences (p < 0.001) among the fifteen genotypes for all the characters studied except for panicle length and 100-grain weight. Highly significant and positive correlations at phenotypic level were observed in grain weight per hill (0.796), filled grains per panicle (0.702), panicles per hill (0.632), and tillers per hill (0.712) with yield per hectare, while moderate positive correlations were observed in flag leaf length to width ratio (0.348), days to flowering (0.412), and days to maturity (0.544). By contrast, unfilled grains per panicle (-0.225) and plant height (-0.342) had a negative significant association with yield per hectare. Filled grains per panicle (0.491) exhibited the maximum positive direct effect on yield followed by grain weight per hill (0.449), while unfilled grain per panicle (-0.144) had a negative direct effect. The maximum indirect effect on yield per hectare was recorded by the tillers per hill through the panicles per hill. Therefore, tillers per hill, filled grains per panicle, and grain weight per hill could be used as selection criteria for improving grain yield in rice.
    Matched MeSH terms: Genotype*
  4. Musa RH, Muhamad NA, Hassan A, Ayob Y, Yusoff NM
    Asian J Transfus Sci, 2015 Jan-Jun;9(1):48-54.
    PMID: 25722573 DOI: 10.4103/0973-6247.150951
    Rh molecular studies have been previously mainly conducted in Caucasians and African population. There is a limited data on the molecular basis for Rh genotypes among Asians.
    Matched MeSH terms: Genotype
  5. Ooi MK, Gan HM, Rohani A, Syed Hassan S
    Genome Announc, 2016;4(4).
    PMID: 27563048 DOI: 10.1128/genomeA.00876-16
    Here, we report the complete genome sequence of a chikungunya virus coinfection strain isolated from a dengue virus serotype 2-infected patient in Malaysia. This coinfection strain was determined to be of the Asian genotype and contains a novel insertion in the nsP3 gene.
    Matched MeSH terms: Genotype
  6. Siti Yazmin Zahari Sham, Nor Aini Umar, Khalidah Mazlan
    With advancement in genetic studies, familial phaeochromocytoma (PCC) and paraganglioma (PGL)
    are increasingly being recognized. Characteristically, correlations exist between genotypes and clinical
    and biochemical phenotypes. We report a phaeochromocytoma in a young patient with intriguing family
    histories, raising the possibility of his being a familial case.
    Matched MeSH terms: Genotype
  7. AR Siti Nurul Fazlin, H Hairul Aini, HM Hadzri, MM Mohammed Imad
    Hepatitis C virus (HCV) genotyping is very important for the clinical management of HCV-infected patients. The aim of this study was to determine the genotypes of HCV-infected patients and to identify their risk factors and comorbidities.
    Matched MeSH terms: Genotype
  8. Kunnath AP, Priyashini T
    Cureus, 2019 Jun 16;11(6):e4907.
    PMID: 31423385 DOI: 10.7759/cureus.4907
    Cancer is one of the greatest threats posed to society, necessitating appropriate diagnosis methods. Modern targeted therapies have greatly advanced the treatment of several solid tumors. The rational use of these agents requires optimal strategies for the rapid and accurate detection of targetable genomic alterations at the time of initial diagnosis and when acquired resistance to targeted therapies develops. Currently used techniques, such as tissue genotyping, have limitations such as difficulty in categorizing tumors, needing frequent sampling, and difficulty in obtaining samples. To overcome these issues, cost-effective and non-invasive methods are an urgent requisite, which would provide an insight into the real-time dynamics of cancers via circulating biomarkers. Circulating tumor DNA (ctDNA), commonly termed "liquid biopsy," has emerged as a new, promising non-invasive tool to detect biomarkers in several cancers. The present review aimed to understand the biological concept of ctDNA and its potential as a biomarker in cancer studies and the clinical utility of this evolutionary diagnostic technique.
    Matched MeSH terms: Genotype
  9. Pyvovar SM, Rudyk IS, Kopytsya MP, Lozyk TV, Galchinskaya VY, Bondar TM
    Pol Merkur Lekarski, 2019 Nov 29;47(281):170-176.
    PMID: 31812970
    The course of heart failure (HF) and its progression is associated with comorbidities, genetic factors and a dynamics of a number of biomarkers. The low triiodothyronine syndrome (LT3S) is observed in some patients with HF. Extremely little data are available in the literature regarding the effect of β-adrenoreceptors (β-AR) genes polymorphisms on the development of LT3S and many contradictory results about their association with HF course. This encourages new research in this area.

    AIM: The aim of study was to evaluate the relationship of β-adrenergic receptors gene polymorphisms with low triiodothyronine syndrome in patients with a heart failure.

    MATERIALS AND METHODS: 354 patients with HF on a background of postinfarction cardiosclerosis were included to the study. At 89 (25.1%) patients LT3S was diagnosed. The course of HF was studied for 2 years. Mean levels of thyroid stimulating hormone (TSH), free T3f and T4f were evaluated. Genotyping of 4 single nucleotide polymorphisms (Gly389Arg of β1-AR gene, Ser49Gly of β1-AR gene, Gln27Glu of β2- AR gene and Ser275 of GNβ3 gene) was performed by polymerase chain reaction. Genetic and epidemiological analysis was performed using the SNPStats program.

    RESULTS: The risk of LT3S in patients with HF increases with homozygous G/G variant of Gln27Glu polymorphism of the β2-AR gene (OR=2.21, p=0.037), described as a recessive model of inheritance. There was a tendency to increase the risk of LT3S development in the presence of the genotype C/T of the Ser275 polymorphism of the GNb3 gene (OR=1.75, p=0.054), described as an over-dominant model. The genotype C/G of the Gln27Glu polymorphism of the β2-AR gene was associated with a decreased risk of LT3S development (OR=0.54, p=0.037), described as over-dominant model. Patients with HF carriers the A allele (A/GA/A) of the Ser49Gly polymorphism of the β1-AR gene have a lower risk of repeated hospitalization due to HF decompensation (OR=0.50, p=0.032), described as a dominant model. There was a tendency to increase the risk of re-hospitalization in the G-allele (C/GG/ G) variant of the Gln27Glu polymorphism of the β2-AR gene (OR=1.68, p=0.057), described as a dominant heredity model. At patients with HF in combination with LT3S the risk of re-hospitalization increases at C/G variant of the Gln27Glu polymorphism of β2-AR gene (OR=1.25, p=0.025), described as an over-dominant model.

    CONCLUSIONS: The results suggest that congenital genetic alterations in β-adrenergic pathways may be associated with the development of LT3S in patients with HF and the features of the HF course.

    Matched MeSH terms: Genotype
  10. Rafii M, Zakiah M, Asfaliza R, Iffah Haifaa M, Latif M, Malek M
    Sains Malaysiana, 2014;43:1-7.
    A total of 17 rice genotypes consisted of 12 F1 progenies and five parental lines were evaluated for performance of grain qualities, yield and yield components and vegetative traits at the Malaysian Agricultural Research and Development Institute (MARDI) Research Station in Seberang Perai, Penang. Except grain length, all other grain quality characters, yield components and vegetative traits varied significantly among the genotypes. Among the studied characters, the highest heritability value was observed in plant height with 99.75% followed by panicle length having 96.90% and grain shape with 56.70% which could be successfully inherited to the next generations. The rest of the traits had low heritability values and ranged from 20.24 to 35.88%. This indicates that the characters are mainly influenced by environmental factors such as geographical effects and climate. However, in the quantitative traits such as grain qualities and yield components, they are usually difficult to inherit to the next generation due to low heritability values. The combinations of Q76 and MR84 had the highest in amylose content while Q76 and MRQ74 had the lowest. Several selected F1 indica rice genotypes from this evaluation are useful for future breeding programme and biotechnological research for the improvement of valuable grain quality traits.
    Matched MeSH terms: Genotype
  11. Ishak NA, Tahir NI, Mohd Sa'id SN, Gopal K, Othman A, Ramli US
    Heliyon, 2021 Feb;7(2):e06048.
    PMID: 33553773 DOI: 10.1016/j.heliyon.2021.e06048
    Recent advances in phytochemical analysis have allowed the accumulation of data for crop researchers due to its capacity to footprint and distinguish metabolites that are present within an organisms, tissues or cells. Apart from genotypic traits, slight changes either by biotic or abiotic stimuli will have significant impact on the metabolite abundances and will eventually be observed through physicochemical characteristics. Apposite data mining to interpret the mounds of phytochemical information from such a dynamic system is thus incumbent. In this investigation, several statistical software platforms ranging from exploratory and confirmatory technique of multivariate data analysis from four different statistical tools of COVAIN, SIMCA-P+, MetaboAnalyst and RIKEN Excel Macro were appraised using an oil palm phytochemical data set. As different software tool encompasses its own advantages and limitations, the insights gained from this assessment were documented to enlighten several aspects of functions and suitability for the adaptation of the tools into the oil palm phytochemistry pipeline. This comparative analysis will certainly provide scientists with salient notes on data assessment and data mining that will later allow the depiction of the overall oil palm status in-situ and ex-situ.
    Matched MeSH terms: Genotype
  12. Harun A
    Malays J Med Sci, 2014 Nov-Dec;21(6):1-2.
    PMID: 25897275
    The emergence of fungal species as opportunistic pathogens has warranted further studies on their pathogenicity, epidemiology, and transmissibility. Fungal genotyping has been employed to study the genetic relatedness within the organism, in order to obtain answers to epidemiological questions (such as in outbreak confirmation) as well as to provide basis for the improvement for patients care. Various fungal genotyping methods have been previously published, which can be chosen depending on the intended use and the capability of individual laboratory.
    Matched MeSH terms: Genotype
  13. Ezhumalai M, Muthanna A, Suhaili Z, Dzaraly ND, Amin-Nordin S, Amal MNA, et al.
    Malays J Med Sci, 2020 Feb;27(1):134-138.
    PMID: 32158353 MyJurnal DOI: 10.21315/mjms2020.27.1.14
    The aim of this study was to study the genotype of a hospital collection of Group B Streptococcus (GBS) from invasive and non-invasive sites. Fifty-one pre-characterised human of GBS were re-identified and further analysed by multilocus sequence typing (MLST) in relation to previously published serotypes. Fifteen sequence types (ST) were found with ST1 being the most predominant. ST1 was also associated with majority of the invasive isolates. The genotypic distribution patterns of GBS in this study were largely in agreement with previous reports from other countries indicating the tendency of certain genotypes to prevail in human infection settings.
    Matched MeSH terms: Genotype
  14. Soon YY, Junaidi I, Kumarasamy V, Chem YK, Juliana R, Chua KB
    Med J Malaysia, 2007 Aug;62(3):214-7.
    PMID: 18246910 MyJurnal
    Since its isolation in Tanzania in 1953, chikungunya virus has caused periodic outbreaks in both tropical Africa and Asia. In the last decade, the virus has shown not only increased activity but has expanded its geographical locations, thus classical delineation of various genotypes of chikungunya virus to specific geographic locales no longer holds true. Rapid mass movement of people and the constant presence of the right vectors in this region could have contributed to the change in virus ecology. This paper documents the first detection of chikungunya virus of Central/East genotype in Malaysia from a patient who was most likely infected with the virus during her visit to India. Without good Aedes vector measures, only time will tell whether this genotype rather than the existing endemic genotype will subsequently cause the next chikungunya outbreak in Malaysia.
    Matched MeSH terms: Genotype*
  15. Ahmad F, Hanafi MM, Hakim MA, Rafii MY, Arolu IW, Akmar Abdullah SN
    PLoS One, 2015;10(9):e0138246.
    PMID: 26393807 DOI: 10.1371/journal.pone.0138246
    Coloured rice genotypes have greater nutritious value and consumer demand for these varieties is now greater than ever. The documentation of these genotypes is important for the improvement of the rice plant. In this study, 42 coloured rice genotypes were selected for determination of their genetic divergence using 25 simple sequence repeat (SSR) primers and 15 agro-morphological traits. Twenty-one out of the 25 SSR primers showed distinct, reproducible polymorphism. A dendrogram constructed using the SSR primers clustered the 42 coloured rice genotypes into 7 groups. Further, principle component analysis showed 75.28% of total variations were explained by the first-three components. All agro-morphological traits showed significant difference at the (p≤0.05) and (p≤0.01) levels. From the dendrogram constructed using the agro-morphological traits, all the genotypes were clustered into four distinct groups. Pearson's correlation coefficient showed that among the 15 agro-morphological traits, the yield contributing factor had positive correlation with the number of tillers, number of panicles, and panicle length. The heritability of the 15 traits ranged from 17.68 to 99.69%. Yield per plant and harvest index showed the highest value for both heritability and genetic advance. The information on the molecular and agro-morphological traits can be used in rice breeding programmes to improve nutritional value and produce higher yields.
    Matched MeSH terms: Genotype*
  16. Tan KK, Nellis S, Zulkifle NI, Sulaiman S, AbuBakar S
    Epidemiol Infect, 2018 10;146(13):1635-1641.
    PMID: 29860959 DOI: 10.1017/S0950268818001425
    Dengue virus type 3 genotype III (DENV-3/III) is widely distributed in most dengue-endemic regions. It emerged in Malaysia in 2008 and autochthonously spread in the midst of endemic DENV-3/I circulation. The spread, however, was limited and the virus did not cause any major outbreak. Spatiotemporal distribution study of DENV-3 over the period between 2005 and 2011 revealed that dengue cases involving DENV-3/III occurred mostly in areas without pre-existing circulating DENV-3. Neutralisation assays performed using sera of patients with the respective infection showed that the DENV-3/III viruses can be effectively neutralised by sera of patients with DENV-3 infection (50% foci reduction neutralisation titres (FRNT50) > 1300). Sera of patients with DENV-1 infection (FRNT50 ⩾ 190), but not sera of patients with DENV-2 infection (FRNT50 ⩽ 50), were also able to neutralise the virus. These findings highlight the possibility that the pre-existing homotypic DENV-3 and the cross-reacting heterotypic DENV-1 antibody responses could play a role in mitigating a major outbreak involving DENV-3/III in the Klang Valley, Malaysia.
    Matched MeSH terms: Genotype*
  17. Rao M, Amran F, Kamaruzaman AA, Hakim Esa HA, Abdul Hameed A, Mohamed Shabery NA
    Am J Trop Med Hyg, 2021 01;104(1):216-218.
    PMID: 33289472 DOI: 10.4269/ajtmh.20-0267
    Leptospirosis is an important zoonotic disease with worldwide distribution and nonspecific clinical manifestation. We report a case of fatal leptospirosis in a previously healthy woman with a causative agent. A young adult Indian woman was brought in dead to the forensic department. Ten days before, she developed fever, dizziness with headache, myalgia, diarrhea, and vomiting. Routine inquest and autopsy were performed on the deceased, revealing hemorrhagic lungs with extensive intra-alveolar hemorrhages, pale liver with dissociation and separation of hepatocyte plates, and edematous brain with histiocyte and lymphocyte infiltration in the parenchyma and meninges. Heart tissue depicts myocarditis and pericarditis inflammatory changes. Cerebrospinal fluid (CSF) was turbid in appearance with mildly elevated leukocytes, predominantly lymphocytes. Real-time PCR targeting lipL32 gene of pathogenic Leptospira was detected in the blood, CSF, brain, kidney, heart, and liver. The genetic profile of the causative agent was ST149 (multi-locus sequence typing Scheme 3). This study illustrates the usefulness of Leptospira PCR assay in postmortem diagnosis and addresses the need for further surveillance to identify the epidemiological link of the disease.
    Matched MeSH terms: Genotype*
  18. Asaduzzaman M, Wahab MA, Rahman MJ, Nahiduzzzaman M, Dickson MW, Igarashi Y, et al.
    Sci Rep, 2019 11 05;9(1):16050.
    PMID: 31690767 DOI: 10.1038/s41598-019-52465-2
    The anadromous Hilsa shad (Tenualosa ilisha) live in the Bay of Bengal and migrate to the estuaries and freshwater rivers for spawning and nursing of the juveniles. This has led to two pertinent questions: (i) do all Hilsa shad that migrate from marine to freshwater rivers come from the same population? and (ii) is there any relationship between adults and juveniles of a particular habitat? To address these questions, NextRAD sequencing was applied to genotype 31,276 single nucleotide polymorphism (SNP) loci for 180 individuals collected from six strategic locations of riverine, estuarine and marine habitats. FST OutFLANK approach identified 14,815 SNP loci as putatively neutral and 79 SNP loci as putatively adaptive. We observed that divergent local adaptations in differing environmental habitats have divided Hilsa shad into three genetically structured ecotypes: turbid freshwater (Western Riverine), clear freshwater (Eastern Riverine) and brackish-saline (Southern Estuarine-Marine). Our results also revealed that genes involved in neuronal activity may have facilitated the juveniles' Hilsa shad in returning to their respective natal rivers for spawning. This study emphasized the application of fundamental population genomics information in strategizing conservation and management of anadromous fish such as Hilsa shad that intersect diverse ecotypes during their life-history stages.
    Matched MeSH terms: Genotype*
  19. Ali MN, Yeasmin L, Gantait S, Goswami R, Chakraborty S
    Physiol Mol Biol Plants, 2014 Oct;20(4):411-23.
    PMID: 25320465 DOI: 10.1007/s12298-014-0250-6
    The present investigation was carried out to evaluate 33 rice landrace genotypes for assessment of their salt tolerance at seedling stage. Growth parameters like root length, shoot length and plant biomass were measured after 12 days of exposure to six different levels of saline solution (with electrical conductivity of 4, 6, 8, 10, 12 or 14 dS m (-1)). Genotypes showing significant interaction and differential response towards salinity were assessed at molecular level using 11 simple sequence repeats (SSR) markers, linked with salt tolerance quantitative trait loci. Shoot length, root length and plant biomass at seedling stage decreased with increasing salinity. However, relative salt tolerance in terms of these three parameters varied among genotypes. Out of the 11 SSR markers RM8094, RM336 and RM8046, the most competent descriptors to screen the salt tolerant genotypes with higher polymorphic information content coupled with higher marker index value, significantly distinguished the salt tolerant genotypes. Combining morphological and molecular assessment, four lanraces viz. Gheus, Ghunsi, Kuthiahara and Sholerpona were considered as true salt tolerant genotypes which may contribute in greater way in the development of salt tolerant genotypes in rice.
    Matched MeSH terms: Genotype
  20. Mm R, Kk W, I I, Zz R, H A
    Pak J Med Sci, 2014 Sep;30(5):1068-71.
    PMID: 25225528 DOI: 10.12669/pjms.305.5224
    Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia.
    Matched MeSH terms: Genotype
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