Affiliations 

  • 1 Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, 02100, Padang Besar, Perlis, Malaysia. johanariff@unimap.edu.my
  • 2 Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, 02100, Padang Besar, Perlis, Malaysia
Dev Genes Evol, 2022 Dec;232(5-6):125-136.
PMID: 36190549 DOI: 10.1007/s00427-022-00697-0

Abstract

Spiders have emerged as one of the leading model organisms in many research fields due to their compelling biology. Often, scientific investigations involving the use of spiders face inevitable problems associated with the lack of specimens from laboratory stock, resulting in difficulties in yielding reproducible investigations for predictive research. Thus, several species of well-studied spiders, including Parasteatoda tepidariorum, have been successfully bred for such purposes. Crossopriza lyoni is a Haplogyne spider, globally distributed and widespread in human inhabitants, prompting interest in various studies over the last decades. Despite its scientific importance, no laboratory-bred C. lyoni has been documented. Therefore, we describe a successful captive breeding system of the species under controlled conditions to establish a laboratory stock culture. Methods for mating induction, egg collection and segregation, artificial embryo incubation, and colony husbandry are discussed. The technique presented is a simple and low-cost approach that is reliable for C. lyoni propagation in the laboratory over several generations.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.