Gingiva Mesenchymal Stem Cells Normoxic or Hypoxic Preconditioned Application Under Orthodontic Mechanical Force on Osterix, Osteopontin, and ALP Expression

Nugraha AP 1 , Narmada IB 1 , Winoto ER 1 , Ardani IGAW 1 , Triwardhani A 1 , Alida A 1 Show all authors , Pramusita A 1 , Nur RM 1 , Indrastie N 1 , Nam HY 2 , Ihsan IS 3 , Riawan W 4 , Rantam FA 5 , Nugraha AP 6 , Noor TNEBTA 7

Affiliations 

  • 1 Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
  • 2 Nanotechnology and Catalysis Research Centre (NANOCAT), Universiti Malaya, Kuala Lumpur, Malaysia
  • 3 Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
  • 4 Biomolecular Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • 5 Laboratory of Immunology and Virology Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • 6 Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
  • 7 Membership of Faculty of Dental Surgery, Royal Collage of Surgeon, Edinburgh University, United Kingdom
Eur J Dent, 2023 Nov 23.
PMID: 37995729 DOI: 10.1055/s-0043-1772699

Abstract

OBJECTIVES:  The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits (Oryctolagus cuniculus) induced with OMF.

MATERIALS AND METHODS:  Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm2 light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 106 cells in a 20 µL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry.

RESULTS:  Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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