Facile engineering of aptamer-coupled silk fibroin encapsulated myogenic gold nanocomposites: investigation of antiproliferative activity and apoptosis induction

Elayappan PK 1 , Kandasamy K 1 , Sasikumar V 2 , Bharathi M 3 , Hirad AH 4 , Alarfaj AA 4 Show all authors , Arulselvan P 5 , Jaganathan R 6 , Ravindran R 6 , Suriyaprakash J 7 , Thangavelu I 8

Affiliations 

  • 1 Department of Biochemistry, Vivekanandha College of Arts and Sciences for Women (Autonomous), Elayampalayam, Tiruchengode, Namakkal, Tamil Nadu, 637205, India
  • 2 Department of Biochemistry, K.S.Rangasamy College of Arts and Science, Tiruchengode, Namakkal, Tamil Nadu, 637215, India
  • 3 Center for Bioinformatics, Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, 641021, India
  • 4 Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box. 2455, 11451, Riyadh, Saudi Arabia
  • 5 Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, 602 105, India
  • 6 Preclinical Department, Faculty of Medicine, Universiti Kuala Lumpur, Royal College of Medicine Perak (UniKL-RCMP), 30450, Ipoh, Perak, Malaysia
  • 7 Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510006, China
  • 8 Department of Chemistry, CHRIST (Deemed to Be University), Bangalore, 560029, India. indumathi.t@christuniversity.in
Biotechnol Lett, 2024 Apr 27.
PMID: 38676857 DOI: 10.1007/s10529-024-03491-2

Abstract

Nanocomposites selectively induce cancer cell death, holding potential for precise liver cancer treatment breakthroughs. This study assessed the cytotoxicity of gold nanocomposites (Au NCs) enclosed within silk fibroin (SF), aptamer (Ap), and the myogenic Talaromyces purpureogenus (TP) against a human liver cancer cell (HepG2). The ultimate product, Ap-SF-TP@Au NCs, results from a three-step process. This process involves the myogenic synthesis of TP@Au NCs derived from TP mycelial extract, encapsulation of SF on TP@Au NCs (SF-TP@Au NCs), and the conjugation of Ap within SF-TP@Au NCs. The synthesized NCs are analyzed by various characteristic techniques. Ap-SF-TP@Au NCs induced potential cell death in HepG2 cells but exhibited no cytotoxicity in non-cancerous cells (NIH3T3). The morphological changes in cells were examined through various biochemical staining methods. Thus, Ap-SF-TP@Au NCs emerge as a promising nanocomposite for treating diverse cancer cells.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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