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Genome Sequencing and Annotation of Mycobacterium tuberculosis PR08 strain

Jaafar MM 1 , Halim MZ 1 , Ismail MI 1 , Shien LL 1 , Kek TL 2 , Fong NY 3 Show all authors , Nor NM 4 , Zainuddin ZF 5 , Hock TT 6 , Najimudin MN 7 , Salleh MZ 1

Affiliations 

  • 1 Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia
  • 2 Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM), Malaysia; Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Malaysia
  • 3 Faculty of Medicine, University of Malaya, Malaysia
  • 4 School of Health Sciences, Health Campus, Universiti Sains Malaysia, Malaysia; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Malaysia
  • 5 School of Health Sciences, Health Campus, Universiti Sains Malaysia, Malaysia
  • 6 Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Malaysia
  • 7 School of Biological Sciences, Universiti Sains Malaysia, Malaysia
Genom Data, 2016 Mar;7:119-20.
PMID: 26981383 DOI: 10.1016/j.gdata.2015.12.030

Abstract

Mycobacterium tuberculosis is an acid fast bacterial species in the family Mycobacteriaceae and is the causative agent of most cases of tuberculosis. Here, we report the genomic features of Mycobacterium tuberculosis isolated from the cerebrospinal fluid (CSF) of a patient diagnosed with both pulmonary and extrapulmonary tuberculosis (TB). The isolated strain was identified as Mycobacterium tuberculosis PR08 (MTB PR08). Genomic DNA of the MTB PR08 strain was extracted and subjected to whole genome sequencing using MiSeq (Illumina, CA,USA). The draft genome size of MTB PR08 strain is 4,292,364 bp with a G + C content of 65.2%. This strain was annotated to have 4723 genes and 48 RNAs. This whole genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number CP010895.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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