Exploration of the diversity of multi-drug resistant Mycobacterium tuberculosis complex in Lagos, Nigeria using WGS: Distribution of lineages, drug resistance patterns and genetic mutations

Noorizhab MNF 1 , Zainal Abidin N 2 , Teh LK 1 , Tang TH 3 , Onyejepu N 4 , Kunle-Ope C 4 Show all authors , Tochukwu NE 4 , Sheshi MA 5 , Nwafor T 6 , Akinwale OP 7 , Ismail AI 8 , Nor NM 9 , Salleh MZ 10

Affiliations 

  • 1 Integrative Pharmacogenomics Institute, Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, Selangor, Malaysia; Faculty of Pharmacy, Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, Selangor, Malaysia
  • 2 Integrative Pharmacogenomics Institute, Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, Selangor, Malaysia
  • 3 Advance Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Kepala Batas, Pulau Pinang, Malaysia
  • 4 Microbiology Department, Nigerian Institute of Medical Research (NIMR), Lagos, Nigeria
  • 5 KNCV Tuberculosis Foundation, Nigeria. Electronic address: msheshi@kncvnigeria.org
  • 6 Public Health and Epidemiology Department, Nigerian Institute of Medical Research (NIMR), Lagos, Nigeria
  • 7 Public Health and Epidemiology Department, Nigerian Institute of Medical Research (NIMR), Lagos, Nigeria. Electronic address: pheabian@yahoo.co.uk
  • 8 Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia
  • 9 School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • 10 Integrative Pharmacogenomics Institute, Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, Selangor, Malaysia. Electronic address: zakisalleh@uitm.edu.my
Tuberculosis (Edinb), 2023 May;140:102343.
PMID: 37080082 DOI: 10.1016/j.tube.2023.102343

Abstract

Multidrug-resistant (MDR) or extensively drug-resistant (XDR) Tuberculosis (TB) is a major challenge to global TB control. Therefore, accurate tracing of in-country MDR-TB transmission are crucial for the development of optimal TB management strategies. This study aimed to investigate the diversity of MTBC in Nigeria. The lineage and drug-resistance patterns of the clinical MTBC isolates of TB patients in Southwestern region of Nigeria were determined using the WGS approach. The phenotypic DST of the isolates was determined for nine anti-TB drugs. The sequencing achieved average genome coverage of 65.99X. The most represented lineages were L4 (n = 52, 83%), L1 (n = 8, 12%), L2 (n = 2, 3%) and L5 (n = 1, 2%), suggesting a diversified MTB population. In term of detection of M/XDR-TB, while mutations in katG and rpoB genes are the strong predictors for the presence of M/XDR-TB, the current study also found the lack of good genetic markers for drug resistance amongst the MTBC in Nigeria which may pose greater problems on local tuberculosis management efforts. This high-resolution molecular epidemiological data provides valuable insights into the mechanistic for M/XDR TB in Lagos, Nigeria.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications