Affiliations 

  • 1 Department of Life Science, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia ; School of Medicine and Public Health, University of Newcastle, Newcastle, NSW 2308, Australia
  • 2 Department of Life Science, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia
  • 3 School of Biomedical Science, University of Newcastle, Newcastle, NSW 2308, Australia
Adv Pharmacol Sci, 2015;2015:164943.
PMID: 26681936 DOI: 10.1155/2015/164943

Abstract

The present study was designed to evaluate the acute and chronic antidepressant effect of genistein in combination with amitriptyline in mice. Animals were divided into six groups (n = 6) for treatment with water, genistein, or amitriptyline, either alone or in combination for ten days. Animals were subjected to locomotor activity testing; tail suspension test (TST); and forced swim test (FST) and immobility time was recorded on day one and day ten. Acute treatment of all treatment groups did not significantly reduce the immobility time (p > 0.05). Chronic treatment of combination of genistein (10 mg/kg) and amitriptyline (5 mg/kg and 10 mg/kg) significantly reduced the immobility time as compared to control group (p < 0.001) and was comparable to amitriptyline alone (10 mg/kg). However, no changes in anti-immobility activity in combination of subeffective doses of genistein (5 mg/kg) and amitriptyline (5 mg/kg) were observed. Genistein at its standard dose (10 mg/kg) rendered synergistic effects in combination with subeffective dose of amitriptyline (5 mg/kg) and additive effects in combination with therapeutic dose of amitriptyline (10 mg/kg).

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.