Rosmarinic acid attenuates inflammation in experimentally induced arthritis in Wistar rats, using Freund's complete adjuvant

Gautam RK 1 , Gupta G 2 , Sharma S 3 , Hatware K 3 , Patil K 3 , Sharma K 4 Show all authors , Goyal S 5 , Chellappan DK 6 , Dua K 7

Affiliations 

  • 1 Department of Pharmacology, MM School of Pharmacy, Maharishi Markandeshwar University, Sadopur-Ambala, India
  • 2 School of Pharmacy, Suresh Gyan Vihar University, Jaipur, India
  • 3 NMIMS, School of Pharmacy & Technology Management, Shirpur, India
  • 4 Department of Pharmacology, Bhupal Nobles' Institute of Pharmaceutical Sciences, Udaipur, India
  • 5 Department of Pharmacology, Mandsaur Institute of Pharmacy, Mandsaur, India
  • 6 Department of Life Sciences, School of Pharmacy, International Medical University (IMU), Bukit Jalil, Malaysia
  • 7 Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI) & School of Biomedical Sciences and Pharmacy, The University of Newcastle (UoN), Callaghan, NSW, Australia
Int J Rheum Dis, 2019 Jul;22(7):1247-1254.
PMID: 31155849 DOI: 10.1111/1756-185X.13602

Abstract

AIM: The purpose of our investigation is to evaluate the anti-arthritic potential of isolated rosmarinic acid from the rind of Punica granatum.

METHOD: Rosmarinic acid was isolated by bioactivity-guided isolation from butanolic fraction of Punica granatum and acute toxicity of rosmarinic acid was carried out. The experiment was conducted at doses of 25 and 50 mg/kg, in Freund's complete adjuvant (FCA)-induced arthritic rats. Various parameters, that is arthritic score, paw volume, thickness of paw, hematological, antioxidant and inflammatory parameters such as glutathione (GSH), superoxide dismutase (SOD), malonaldehyde (MDA) and tumor necrosis factor-α (TNF-α) were also estimated.

RESULTS: Rosmarinic acid significantly decreased the arthritic score, paw volume, joint diameter, white blood cell count and erythrocyte sedimentation rate. It also significantly increased body weight, hemoglobin and red blood cells. The significantly decreased levels of TNF-α were observed in treated groups as compared to arthritic control rats (P 

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