Chikungunya Virus Infection of Aedes Mosquitoes

Wong HV 1 , Chan YF 1 , Sam IC 1 , Sulaiman WY 2 , Vythilingam I 3

Affiliations 

  • 1 Department of Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia
  • 2 Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia
  • 3 Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia. indrav@um.edu.my
Methods Mol Biol, 2016;1426:119-28.
PMID: 27233266 DOI: 10.1007/978-1-4939-3618-2_11

Abstract

In vivo infection of mosquitoes is an important method to study and characterize arthropod-borne viruses. Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that is transmitted primarily by Aedes mosquitoes. In this chapter, we describe a protocol for infection of CHIKV in two species of Aedes mosquitoes, Aedes aegypti and Aedes albopictus, together with the isolation of CHIKV in different parts of the infected mosquito such as midgut, legs, wings, salivary gland, head, and saliva. This allows the study of viral infection, replication and dissemination within the mosquito vector.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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