The aim of this project is to produce electricity from citronella biomass using isolated microbes from wastewater as biocatalyst in a dual chamber microbial fuel cell (MFC). MFC is one such system that not only reduced biomass, which contains mostly waste products but can also liberate electricity from them. MFC system is well-established and using lignocellulosic biomass as fuel is one step to future energy generation. Trials of MFC experiments have been conducted but using citronella bagasse (CB) as fuel source. Furthermore, pre-treatment of the biomass was done using NaOH pre-treament and effluent treatment wastewater from a palm edible oil company as a source for microorganism. The end results indicate that bioelectricity production from CB is possible though very low yield in the present MFC.