Improvement of tissue culture, genetic transformation, and applications of biotechnology to Brassica

Ravanfar SA 1 , Orbovic V 2 , Moradpour M 3 , Abdul Aziz M 3 , Karan R 1 , Wallace S 4 Show all authors , Parajuli S 5

Affiliations 

  • 1 a Department of Agronomy , Institute of Food and Agricultural Sciences, University of Florida , Gainesville , FL 32611-0300 , USA
  • 2 b Citrus Research and Education Center-University of Florida/IFAS , Lake Alfred , FL 33850 , USA
  • 3 d Laboratory of Plantation Crops , Institute of Tropical Agriculture, Universiti Putra Malaysia , 43400 Serdang , Selangor Darul Ehsan , Malaysia
  • 4 c Department of Biology , University of Iowa , Iowa City , IA 52242-1324 , USA
  • 5 e Gulf Coast Research and Education Center, University of Florida , Wimauma , FL 33598 , USA
Biotechnol Genet Eng Rev, 2017 Apr;33(1):1-25.
PMID: 28460558 DOI: 10.1080/02648725.2017.1309821

Abstract

Development of in vitro plant regeneration method from Brassica explants via organogenesis and somatic embryogenesis is influenced by many factors such as culture environment, culture medium composition, explant sources, and genotypes which are reviewed in this study. An efficient in vitro regeneration system to allow genetic transformation of Brassica is a crucial tool for improving its economical value. Methods to optimize transformation protocols for the efficient introduction of desirable traits, and a comparative analysis of these methods are also reviewed. Hence, binary vectors, selectable marker genes, minimum inhibitory concentration of selection agents, reporter marker genes, preculture media, Agrobacterium concentration and regeneration ability of putative transformants for improvement of Agrobacterium-mediated transformation of Brassica are discussed.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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