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Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates

Khatun M 1 , Alam SMS 1 , Khan AH 2 , Hossain MA 1 , Haq JA 3 , Alam Jilani MS 3 Show all authors , Rahman MT 4 , Karim MM 5

Affiliations 

  • 1 Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • 2 Department of Medicine, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000, Bangladesh
  • 3 Department of Microbiology, Ibrahim Medical College & BIRDEM Hospital, Dhaka 1000, Bangladesh
  • 4 Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia
  • 5 Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh. Electronic address: manjur@du.ac.bd
Asian Pac J Trop Med, 2017 Aug;10(8):753-759.
PMID: 28942823 DOI: 10.1016/j.apjtm.2017.08.002

Abstract

OBJECTIVE: To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates.

METHODS: Peripheral blood, bone marrow and spleen aspirate samples were collected from clinically suspected VL patients (n = 26). A new PCR primer pair (MK1F/R) was designed targeting kinetoplast mini circle DNA sequences of Leishmania donovani, and Leishmania infantum, and was used to diagnose VL along with some other established primers for VL in polymerase chain reactions. Test was validated by comparing with several other diagnostic methods.

RESULTS: The designed primer set showed 100% specificity and 98% sensitivity in detecting VL using blood samples, when compared with more invasive samples: bone marrow or spleen aspirates.

CONCLUSIONS: The newly designed primer MK1F/R could be a better alternative for PCR based diagnosis of VL using less invasive sample, peripheral blood instead of bone marrow or spleen aspirates.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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