Affiliations 

  • 1 1Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, 11800 Gelugor, Pulau Pinang Malaysia
  • 2 2Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia
  • 3 4Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia
  • 4 5Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Cheras Kuala Lumpur, Malaysia
  • 5 6School of Engineering, Taylor's University, No. 1 Jalan Taylor's, 47500 Subang Jaya, Selangor Malaysia
3 Biotech, 2018 Jun;8(6):288.
PMID: 29938157 DOI: 10.1007/s13205-018-1295-y

Abstract

This study aimed at recovery of thermostable lipase from Escherichia coli BL21 using porous glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system (AIRS). The influencing parameters such as concentration and pH of extraction solution, concentration of NaCl, size of the beads, and pH of the desorption solution on the partition behaviour of lipase were evaluated. Smaller adsorbent (4 mm) had a 65.5% of recovery yield with approximately two-fold higher purification factor compared to that obtained with the larger adsorbent. Recombinant lipase was purified successfully using AIRS with a purification factor of 7.6 and yield of 78.4% under optimum conditions of 18% (w/w) PEG 4000, 10% (w/w) of potassium citrate at pH 9 with 3% (w/w) of NaCl. Optimum desorption was obtained with 4.0 mm of porous glass beads at pH 9.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.