Global Metabolic Analyses of Acinetobacter baumannii

Mahamad Maifiah MH 1 , Velkov T 2 , Creek DJ 3 , Li J 4

Affiliations 

  • 1 International Institute for Halal Research and Training, International Islamic University Malaysia, Gombak, Selangor, Malaysia
  • 2 Department of Pharmacology and Therapeutics, The University of Melbourne, Parkville, VIC, Australia
  • 3 Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia
  • 4 Laboratory of Antimicrobial Systems Pharmacology, Department of Microbiology, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia. Jian.Li@monash.edu
Methods Mol Biol, 2019;1946:321-328.
PMID: 30798566 DOI: 10.1007/978-1-4939-9118-1_28

Abstract

Acinetobacter baumannii is rapidly emerging as a multidrug-resistant pathogen responsible for nosocomial infections including pneumonia, bacteremia, wound infections, urinary tract infections, and meningitis. Metabolomics provides a powerful tool to gain a system-wide snapshot of cellular biochemical networks under defined conditions and has been increasingly applied to bacterial physiology and drug discovery. Here we describe an optimized sample preparation method for untargeted metabolomics studies in A. baumannii. Our method provides a significant recovery of intracellular metabolites to demonstrate substantial differences in global metabolic profiles among A. baumannii strains.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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